+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-11854 | |||||||||
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Title | Structure of the human THO - UAP56 complex (Map C) | |||||||||
Map data | Map C sharpened (Map sharpening B-factor -160 A%u030A2) | |||||||||
Sample |
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Biological species | Homo sapiens (human) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.7 Å | |||||||||
Authors | Puehringer C / Hohmann U / Plaschka C | |||||||||
Citation | Journal: Elife / Year: 2020 Title: Structure of the human core transcription-export complex reveals a hub for multivalent interactions. Authors: Thomas Pühringer / Ulrich Hohmann / Laura Fin / Belén Pacheco-Fiallos / Ulla Schellhaas / Julius Brennecke / Clemens Plaschka / Abstract: The export of mRNA from nucleus to cytoplasm requires the conserved and essential transcription and export (TREX) complex (THO-UAP56/DDX39B-ALYREF). TREX selectively binds mRNA maturation marks and ...The export of mRNA from nucleus to cytoplasm requires the conserved and essential transcription and export (TREX) complex (THO-UAP56/DDX39B-ALYREF). TREX selectively binds mRNA maturation marks and licenses mRNA for nuclear export by loading the export factor NXF1-NXT1. How TREX integrates these marks and achieves high selectivity for mature mRNA is poorly understood. Here, we report the cryo-electron microscopy structure of the human THO-UAP56/DDX39B complex at 3.3 Å resolution. The seven-subunit THO-UAP56/DDX39B complex multimerizes into a 28-subunit tetrameric assembly, suggesting that selective recognition of mature mRNA is facilitated by the simultaneous sensing of multiple, spatially distant mRNA regions and maturation marks. Two UAP56/DDX39B RNA helicases are juxtaposed at each end of the tetramer, which would allow one bivalent ALYREF protein to bridge adjacent helicases and regulate the TREX-mRNA interaction. Our structural and biochemical results suggest a conserved model for TREX complex function that depends on multivalent interactions between proteins and mRNA. | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_11854.map.gz | 303.7 MB | EMDB map data format | |
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Header (meta data) | emd-11854-v30.xml emd-11854.xml | 12.3 KB 12.3 KB | Display Display | EMDB header |
Images | emd_11854.png | 13.7 KB | ||
Others | emd_11854_additional_1.map.gz | 300.4 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-11854 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-11854 | HTTPS FTP |
-Validation report
Summary document | emd_11854_validation.pdf.gz | 253 KB | Display | EMDB validaton report |
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Full document | emd_11854_full_validation.pdf.gz | 252.1 KB | Display | |
Data in XML | emd_11854_validation.xml.gz | 7.2 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-11854 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-11854 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_11854.map.gz / Format: CCP4 / Size: 325 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Map C sharpened (Map sharpening B-factor -160 A%u030A2) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.34 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Additional map: Map C unsharpened
File | emd_11854_additional_1.map | ||||||||||||
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Annotation | Map C unsharpened | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
-Entire : THO - UAP56
Entire | Name: THO - UAP56 |
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Components |
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-Supramolecule #1: THO - UAP56
Supramolecule | Name: THO - UAP56 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#14 |
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Source (natural) | Organism: Homo sapiens (human) |
Recombinant expression | Organism: Trichoplusia ni (cabbage looper) / Recombinant strain: Hi5 |
Molecular weight | Theoretical: 1.836 MDa |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.9 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 2 / Number real images: 26303 / Average exposure time: 3.8 sec. / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: OTHER / Imaging mode: OTHER |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
+Image processing
-Atomic model buiding 1
Refinement | Space: REAL / Protocol: AB INITIO MODEL |
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