EMDB-10175: Head domain of the mt-SSU assemblosome from Trypanosoma brucei

Basic information

• Entry: Database: EMDB / ID: EMD-10175
• Title: Head domain of the mt-SSU assemblosome from Trypanosoma brucei
• Map data: Head domain of the mt-SSU assemblosome of Trypanosoma brucei brucei

Sample

• Complex: Cryo-EM structure of the head domain of the mt-SSU assemblosome from Trypanosoma brucei
  • Protein or peptide: x 52 types
  • RNA: x 1 types
• Ligand: x 5 types

Function / homology

Function:

rRNA (cytosine-N4-)-methyltransferase activity / mitochondrial mRNA editing complex / mitochondrial RNA processing / thiosulfate sulfurtransferase activity / kinetoplast / rRNA base methylation / mRNA stabilization / nuclear lumen / ciliary plasm / mitochondrial small ribosomal subunit / S-adenosylmethionine-dependent methyltransferase activity / Transferases; Transferring one-carbon groups; Methyltransferases / methyltransferase activity / methylation / mitochondrial matrix / structural constituent of ribosome / translation / mitochondrion / RNA binding / cytoplasm

Domain/homology:

Mitochondrial glycoprotein / Mitochondrial glycoprotein superfamily / Mitochondrial glycoprotein / Ribosomal RNA small subunit methyltransferase H / S-adenosyl-L-methionine-dependent methyltransferase, MraW, recognition domain superfamily / MraW methylase family / Ribosomal protein Rsm22-like / Mitochondrial small ribosomal subunit Rsm22 / Ribosomal protein S23/S29, mitochondrial / Mitochondrial ribosomal death-associated protein 3 / Rhodanese-like domain / Rhodanese domain profile. / Rhodanese-like domain superfamily / Rhodanese-like domain / Ribosomal protein S9 / Ribosomal protein S9/S16 / Ribosomal protein S11 superfamily / Thioredoxin-like superfamily / Ribosomal protein S5 domain 2-type fold, subgroup / Ribosomal protein S5 domain 2-type fold / S-adenosyl-L-methionine-dependent methyltransferase superfamily

Component:

Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Methyltransferase / Mitochondrial SSU ribosomal protein / Mitochondrial SSU ribosomal protein / CENP-V/GFA domain-containing protein / Uncharacterized protein / Alpha/beta-Hydrolases superfamily protein / Uncharacterized protein / Uncharacterized protein / Glutaredoxin domain-containing protein / S-adenosyl-methyltransferase mraW-like protein, putative / Ribosomal protein S9 / Uncharacterized protein / Mitochondrial glyco protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Small ribosomal subunit protein mS29 / Rhodanese domain-containing protein

• Biological species: Trypanosoma brucei brucei (eukaryote) / Trypanosoma brucei brucei
• Method: single particle reconstruction / cryo EM / Resolution: 3.1 Å
• Authors: Saurer M / Ramrath DJF / Niemann M / Calderaro S / Prange C / Mattei S / Scaiola A / Leitner A / Bieri P / Horn EK / Leibundgut M / Boehringer D / Schneider A / Ban N

Funding support

Switzerland, 1 items

Organization	Grant number	Country
Swiss National Science Foundation		Switzerland

• Citation: Journal: Science / Year: 2019; Title: Mitoribosomal small subunit biogenesis in trypanosomes involves an extensive assembly machinery.; Authors: Martin Saurer / David J F Ramrath / Moritz Niemann / Salvatore Calderaro / Céline Prange / Simone Mattei / Alain Scaiola / Alexander Leitner / Philipp Bieri / Elke K Horn / Marc Leibundgut / Daniel Boehringer / André Schneider / Nenad Ban / ; Abstract: Mitochondrial ribosomes (mitoribosomes) are large ribonucleoprotein complexes that synthesize proteins encoded by the mitochondrial genome. An extensive cellular machinery responsible for ribosome assembly has been described only for eukaryotic cytosolic ribosomes. Here we report that the assembly of the small mitoribosomal subunit in involves a large number of factors and proceeds through the formation of assembly intermediates, which we analyzed by using cryo-electron microscopy. One of them is a 4-megadalton complex, referred to as the small subunit assemblosome, in which we identified 34 factors that interact with immature ribosomal RNA (rRNA) and recognize its functionally important regions. The assembly proceeds through large-scale conformational changes in rRNA coupled with successive incorporation of mitoribosomal proteins, providing an example for the complexity of the ribosomal assembly process in mitochondria.

History

Deposition	Aug 3, 2019	-
Header (metadata) release	Sep 18, 2019	-
Map release	Sep 18, 2019	-
Update	Sep 25, 2019	-
Current status	Sep 25, 2019	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_10175.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Head domain of the mt-SSU assemblosome of Trypanosoma brucei brucei
• Voxel size: X=Y=Z: 1.39 Å

Density

Contour Level	By AUTHOR: 0.0793 / Movie #1: 0.0793
Minimum - Maximum	-0.18639 - 0.4196252
Average (Standard dev.)	0.0011354706 (±0.011508951)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 56 56 56 Dimensions 320 320 320 Spacing 320 320 320
Cell	A=B=C: 444.8 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.39	1.39	1.39
M x/y/z	320	320	320
origin x/y/z	0.000	0.000	0.000
length x/y/z	444.800	444.800	444.800
α/β/γ	90.000	90.000	90.000
MAP C/R/S	1	2	3
start NC/NR/NS	56	56	56
NC/NR/NS	320	320	320
D min/max/mean	-0.186	0.420	0.001

Supplemental data

Sample components

Entire : Cryo-EM structure of the head domain of the mt-SSU assemblosome f...

• Entire: Name: Cryo-EM structure of the head domain of the mt-SSU assemblosome from Trypanosoma brucei

Components

• Complex: Cryo-EM structure of the head domain of the mt-SSU assemblosome from Trypanosoma brucei
  • Protein or peptide: uS5m
  • Protein or peptide: uS11m
  • Protein or peptide: mS38
  • Protein or peptide: mt-SAF18
  • Protein or peptide: mt-SAF28
  • Protein or peptide: mt-SAF30
  • RNA: 9S rRNA
  • Protein or peptide: uS3m
  • Protein or peptide: uS9m
  • Protein or peptide: uS10m
  • Protein or peptide: uS14m
  • Protein or peptide: uS19m
  • Protein or peptide: mS29
  • Protein or peptide: mS33
  • Protein or peptide: mS35
  • Protein or peptide: mS49
  • Protein or peptide: mS50
  • Protein or peptide: mS52
  • Protein or peptide: mS53
  • Protein or peptide: mS54
  • Protein or peptide: mS55 (KRIPP8)
  • Protein or peptide: mS57
  • Protein or peptide: mS58
  • Protein or peptide: mS67
  • Protein or peptide: mS69
  • Protein or peptide: mS70
  • Protein or peptide: mS71
  • Protein or peptide: mS72
  • Protein or peptide: mt-SAF1 (RSM22)
  • Protein or peptide: mt-SAF4
  • Protein or peptide: mt-SAF12 (KRIPP18)
  • Protein or peptide: mt-SAF14
  • Protein or peptide: mt-SAF15
  • Protein or peptide: mt-SAF16
  • Protein or peptide: mt-SAF17
  • Protein or peptide: mt-SAF19
  • Protein or peptide: mt-SAF20
  • Protein or peptide: mt-SAF25
  • Protein or peptide: mt-SAF34
  • Protein or peptide: UNK-a
  • Protein or peptide: UNK-b
  • Protein or peptide: UNK-c
  • Protein or peptide: UNK-d
  • Protein or peptide: UNK-e
  • Protein or peptide: UNK-f
  • Protein or peptide: UNK-g
  • Protein or peptide: UNK-h
  • Protein or peptide: UNK-i
  • Protein or peptide: UNK-j
  • Protein or peptide: UNK-k
  • Protein or peptide: UNK-l
  • Protein or peptide: UNK-m
  • Protein or peptide: UNK-x
• Ligand: GUANOSINE-5'-TRIPHOSPHATEGuanosine triphosphate
• Ligand: MAGNESIUM ION
• Ligand: ZINC ION
• Ligand: S-ADENOSYL-L-HOMOCYSTEINE
• Ligand: water

Supramolecule #1: Cryo-EM structure of the head domain of the mt-SSU assemblosome f...

• Supramolecule: Name: Cryo-EM structure of the head domain of the mt-SSU assemblosome from Trypanosoma brucei; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#53
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427

Macromolecule #1: uS5m

• Macromolecule: Name: uS5m / type: protein_or_peptide / ID: 1 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 50.147355 KDa

Sequence

String:

MFRLTAASQK NLNSWYTKGT MRGGVPRIYY AWMRPGSFTR RRFEKMRNPF VDLETGTSLY FRDTRDSAEA IAHAADSKGI KGMDNAIDL YNEYRIVPDL YPEGFQWKHK LNTEYNQWRS NTWLTPDLIP KEHRGRFLCN FQLNIVAYDM RVVKFSPKDH R QWIYCVLY VGSGKGIAGW GRAVAPSTQE AKKEAIREAF SNIIAVDLEQ EGPMYPVRVN ADGVRVLLYP ARRIVANFRV AD ILCAFGF QHAGCRINLK ATNNPKSPTH TVEGVFEAVK ALRSVSEIAA SRGKVPHSLI YNIYPYLEEI RRRKGMMAMH PPG KDGLLM PDRVVDNRLP DHLKRGYYDD VYWKDFFAGS DEHLNEPRMG LRGDEMRRRL EEAQTSPAPT TAKDTRRRTL EDVL KRLGK TTRDLGSIPI VNPRVDVGLP THIKRNYQLH

Macromolecule #2: uS11m

• Macromolecule: Name: uS11m / type: protein_or_peptide / ID: 2 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 37.953965 KDa

Sequence

String:

MMRKSVTFLR RGKPRPRAGM FPDKYRRVPM LLKPQQGGQQ YFNHFLIRST NDRLTQQDVD NASGQAHSFI SPQLPQMDWR NMSARSSEE SIREEMRQLA ENDVMQHQRV FNERMWYEKE EEHRMKARSC PEETSEHAIS NDGAVPPPRV LGGDYFKTRF G YSLVKNSE MTQGPVDYSQ LDMWGEMPRY TSDMVFLYLV SRRRNTYAVA YTYEGKRILN TYTAGNRGLK GGDRGFRSEG ST DNGHQVT SMYLNDLLPK LREMRASEGR PMGRGEKVEL VVRVMGFYNG RQGAVRAVQD RANEFHVRYF EDITPFPLNG PKM PRGVFK

Macromolecule #3: mS38

• Macromolecule: Name: mS38 / type: protein_or_peptide / ID: 3 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 28.2406 KDa

Sequence

String:

MRAGCVACSR IPKLGIAALG STTTSMQATC QETQELRCAT TQMAPAVIFP LPPPLRGSYI DRKPTAASFN EHHATASFRH HMIASADVS HRPRHFYFAS GTRNDTGTRS VKEGERKQLK QQTNVEVDSV AYGDQLDELS ARWAAKFYGQ VTFGPRNYPY P SSRWLARR FQMKKHRIIK RFRFRRYKLA AVANLPFAKM IRVGMLPELK SSKTKRGDVV DPTLSGQLVS AVKNTGEKRK GQ RTRPKSK YQV

Macromolecule #4: mt-SAF18

• Macromolecule: Name: mt-SAF18 / type: protein_or_peptide / ID: 4 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 42.508039 KDa

Sequence

String:

MLCHNRLLLV NKQTQKYRTK LRYRFRQPSV VPLRQTLQQR HNTILEVLRR RRINSGDQSP YRYVEERLYS KPSRLDREGV KVNKTYALQ GLGDLEPLRY GANFGISEKD ALKYETVAEK AKYMEPPIPY SSLAARKLAA GALWPAAPDP EGMISKEVRL L RHESSMSP SARAFSERVA YHLRRSLKAC PGHIAEHIDF TQLIIQEVLG SRRSKEIYIV WFTVDPGARF ELEPRLHQLN HW VQQLIIK RVKRRPHIPR VTWIYDGGRL ERELPRDVKQ ELQSFVADAA TTLESRVKYL KELDTMNQRM KDIPWFMPYL WSK EEKAAR QKSMLADLEE VERRKNEHSS GRSAPPRTSP PPQFVR

Macromolecule #5: mt-SAF28

• Macromolecule: Name: mt-SAF28 / type: protein_or_peptide / ID: 5 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 21.468707 KDa

Sequence

String:

MWRLSRSLRS NSLHNPGPFL DGALQLIKLH LAHKNAAADK NTKACSDIEG EFLRELEAFR ACFTMSSSLK VAKLYTKKLH GALSYFQLY DDPLMRQLDM IIGKQTMQPS AGRQHGVFKA PVAARLDPFF LDEREETVLP SELPNPPKPD PSTPLRERAL K VPAQHRGH WVLRDPDIAI TREERRTDPW

Macromolecule #6: mt-SAF30

• Macromolecule: Name: mt-SAF30 / type: protein_or_peptide / ID: 6 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 19.659756 KDa

Sequence

String:

MPPNSATRWL PFVSSDLKDY LNRYWAVMFT VGARPIETGH IRHYVSWYCT RMKVVLLDHH VYVEPLRQQL QEASRTPELP LLFVNKKLV GTLRDVELLE REKKLKDVLH FGFEWRVGGS VAATNGQKSL MGALPAPYGD AEFFRGRYRG PPVARPVVSL P TLHPFALR SEE

Macromolecule #8: uS3m

• Macromolecule: Name: uS3m / type: protein_or_peptide / ID: 8 ; Details: uS3m of Trypanosoma brucei; encoded on mitochondrial DNA; GenBank: M94286.1; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 9.07094 KDa

Sequence

String:

MFLIHFVHYK TILQKYTFKF KHIFLSIDKY NSLFFNISGI LIWLNIIHIN IILIKYSFFI LINNFEYLII LIST

Macromolecule #9: uS9m

• Macromolecule: Name: uS9m / type: protein_or_peptide / ID: 9 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 50.201016 KDa

Sequence

String:

MQKLLSPRTA RHARLFRLAG KLADSGSPGV PKSDGERLVW VNSHVRRDKD ISLSQEEERI RELMMPLEVG ENSFAANGQA THGNLFYFR EYPMYPGEYV PAEHNTLSSL RDELRLDLTA QSLKEAWMRV SGGVYFQSVD EYYASVDGLD AEQIGEVLAA L FPELNCYE AQALVQRTLE CISRPVSAAS RQLSRTITAE AVGLDNAPGH YTNFLEWMGR LTETRAFKTE HALFEFSRRK FN RDDVRVM FENYRLMSKA TLLADSADSY SHFYTVLKDF ARKVAGEDSR HQIGVRIDEA EVDPETGIAV GRGCADGEKY HFT ALLREN RDHNGIITVM GKPLSLVLDN KAWLMEMVLM PFDEANLDYR DFDAHIVSEG HAMPSIANEI AAFALRMAVA NALV KLIPL TRIPLKKSGL LSVDRRRERG QFPGYLDGKK VKRRFAKR

Macromolecule #10: uS10m

• Macromolecule: Name: uS10m / type: protein_or_peptide / ID: 10 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 94.321055 KDa

Sequence

String:

MVRRSHVVAY YWSRYRMPTQ MPKFDGPAPV AAPQSMNSTK TNEFIDPIDD KFPMSIRGPL VRPDVPEDQY VDSWYICTSM THHMGDYRP WSASAPPNAF RFRPFNEFDA KGREYVQYMR EFARFDPRKS RGNGQKGFPF RDAYLTKMNE ANQKTPPPTL E TIMDRAVR EHHQHARILS PLEVQRDVGR LEPIPSYAGK INADRSVFPF QWKTEDWYEY EVAKVRNRRF VFENTEEDGI RG SEVTYKI VLEGFWDHHV MKLAEDVCMF LKDVGRQIVE EKLVAVRRLL QGGAVDPELL AAFNCARAGP FGGLDEYDKE EVA NFLRSD LRRLEEQCLS VINRCNVPVP GATNIYDPHT SWPHVEKLEP WVRMAEFWTS SSDTSFTELE MSTAHYEFRK FFRV IICKL PFQSTEFEKR MYDIRHWLHR QTSCEFHTIY RRNVIHDSAV FPTEHDPATP TTHEHHRMFS FALDWQSAPV NRLST DTVH EGESWDAVAQ RLGCSVGELK DANAERETIE AGVVINVPVT ATRRLTSFGA TPLVLPLKTT SAKDGERIRT WEEAAA ILD CTVEELQQCN GHAALTYQKK ESEAGEFDSS VTELVAPLSC WTSTSESEFS PVERVHANDT LVAIARRLQC SEEALRA VN DGITDVSGLD FVRVPPEARR PRRLVEPQLR PQAATDALLA RTIAEEETFK LKSIPHLPQN AERFPHEYHT PTSRFPPT P SETPATQDWM AYTAKYLDKQ FTISAEPAPV YNVNKLWPMQ QIPGKVDQTP FEEDQTWLLH SIPVQQLEMH HHEKDLQDL PFINHEQFPR SLEWNAP

Macromolecule #11: uS14m

• Macromolecule: Name: uS14m / type: protein_or_peptide / ID: 11 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 19.900014 KDa

Sequence

String:

MFCFSRGLWM RHIGQDVPKR HTHFVLESRL MYEKSFRDCW LHSVCRAISQ LDEPLSKTVV GTHQKMLQRK VTCFQYNQYG LFKTPYYRL ANVDRYHAVQ GVAGTREWVP YVNVSYWTMN KMVRGGNLLV HRVHYTGWGT DSHLKKGGWE HRWNKVLQRN V LQYSRI

Macromolecule #12: uS19m

• Macromolecule: Name: uS19m / type: protein_or_peptide / ID: 12 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 28.364604 KDa

Sequence

String:

MAFRNTFTTP GKFSTVSKNI VLLLIWRVKV FLRAEGFAHS LVMLPVSLYS KILLCDVKKK IVYFHCCTRK KSMLRRCPCV WPDGPTKSS VSIGTAFTLQ KRFLKIAKSA FGFYLARRGQ RKYPFLRRPH IKNTHSMNPS APYFWSFMTA KSQMAFLPEE N YITGDWTG KFFVSKRQVY TLQHATSGAK VRVKSFPSIF EFNSPSRWNI GKEMNTLTKP RMDLIDEQML TKKQRLDYVR AG LLPK

Macromolecule #13: mS29

• Macromolecule: Name: mS29 / type: protein_or_peptide / ID: 13 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 57.048836 KDa

Sequence

String:

MRSKTLKKSL AAMYMRPPVT CYTDACEAPV AMWDGAIPLK ETRKLKNGVP VRTVSRTYSH PPQLTPTQLS FNDINSMYCV GNDELIQFF PEGLGGRVFQ TMPPGHPRGF LYRKETHLLN LFVDKVQHWH TKRSVLSSLT NGRTGFIVDG PTGCGKSALM C QVVHFARS RNIVTLYVPD AKVWTHGEWC WPSTILPGFF DAPDAARSFL KYFAVANRAT LTSWKLRCTP KDLPTEQGER QP QNLYELC EWGHRAVAPA SIDRQSVCVK FLMDELSEEK KLPVVIVVDG WNLFSHETHF RYPHPDFLRG LASFNESSTD IDL YPQELP RIPASRLSFV RGLNKMILSG DDPNKFFITC TTRDFKPFDG ISGFPNVETD RFANSLDEYA PYDPEKDSHF HPIQ IGNFD EYEYRSFLRF LINSGELAGL GWGPLWHASS DFERKLYKIG FLSGRNPQGV VDHYHQELVW RYDYQRTRQK QYLLK RRME GMSRGASSGA VGVR

Macromolecule #14: mS33

• Macromolecule: Name: mS33 / type: protein_or_peptide / ID: 14 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 21.244104 KDa

Sequence

String:

MVLRRWFPLL GVHRVGYTHP STLPVPCAQR WDLRLARARI FQEYIEEKAP GAWQLEDERS MSPEFKTFTG YPMRDMRPGY GQNLPDYIM KKRLPNNTHY ELFARRDIPN EDNAMYGKYL YDMTVHGTSL PSTYRMHKDI NKAQRNDRKL SGNRFKVLCS S GAKNPPSG WEPIPDATEE EED

Macromolecule #15: mS35

• Macromolecule: Name: mS35 / type: protein_or_peptide / ID: 15 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 98.377773 KDa

Sequence

String:

MRRWHRSLNP ALFGERGSGH YFSTTRARLQ LQAQDRIICG THGAGIVAHQ TAVNSSDNPM LSSVTTAAPA ADLSRTKPHE GTGTSERDP YIRTLHNQRS AAPESSVSQS HTVNAPTVDE CEMLAERWGT MNYWHNDTFP RLVVFLKKLL VPDVSPLSPT A ESLLSMFE KVVIPKLTSD EEDRRKLVSL WSETTLQAEA AVTKFLFQRG SFESMLHRII TDALEKMSTL ALGGQEGNLA LE ALKRQTL FKRNDYIQKR LIDVVSNSAY LGYGDSVWQV FFAAVEANEE NLLSDRATTD AIRAAWEGVM REDVVRLPDV TGV VALYLT LVCIRESGRL VPEELKELSS GLEDGVRPGV RKLQQYPLIF LHPTVKRRFV VKAVAEILHN SSSNAFSNML RENG LHDTA REVALCEAMN RNKELAEGDV GDAVGRFVSK GEVKTLLSSL VSGTDAVVRD AVAGIFGIGT TITIDWDAVM QNVDW SNNW QRLATALLSN SAVLSAIVKL VKNAIGAKGM SKHLFTDEYA DQLQLILDAR EERAASRKQR IENIAQELSS FERVDL SCD LLRKLGVDMT ELDTAAAATR NMNVVQRPCI EDGLLSLVLE AVTKRHPNWV KAGVIQTTLK DPFDALRWMM HIFIRLS YV PHAGAATIAR LSRRRIGPIG LEPHQFNVPA ELGFVEQYDN LQYKRYDWQG WYQRMLDVHN RNVSLRCRIC DLQRLDGN G VQFVDMQTER RLRILAQHRV GMGVLKLDAD KYEDQADNVT FGTTKLSELL ADARKAQLGE EYWPSVELKV RKPSGQSKA HYSLIDNERI EKRSRELYEK YRDAKKRSLF VTPMETWLEV KGMQVRKSVD NADEDGYTLD ALQDMMDGDD GDKV

Macromolecule #16: mS49

• Macromolecule: Name: mS49 / type: protein_or_peptide / ID: 16 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 137.234125 KDa

Sequence

String:

MIRRRVCDGA RLAPNIRATF SAVRYQSGLH TFIRDSKPSN FSSVRRSENA NGDATASPGA TAGENPASSG DWASHMQREL FGEVDPLGG QAHKDYYRDV TRGYSPQYAP RNFANGGAVA YPHIQSPYEY EEAAHRRVWL DHDVDRMREE FTQHRASLRS L ASAQEREE LLRSRAAEYQ VANTVHESES VHPIQQLYNS GGTSRSALKQ QAVADRYSIA EQHSPLPLTT GVDRDALDEA QR TKDRILN DSFTAENLLI THGLREKEKH DFTILQRTVR IPFQGYDMDR FLAQQKGTPY GAQQLPPNVV PSSMEEAQRT LRG SSATAT PLVDAVAQKV YARNTVVDRP AIGEQLTEQI INIMRASRTT AEQQREEERA QRFGLGRQGA LVQDGGPDQR TLKK HTNDE RIVDAMLFQQ NAYRKTPTDE HWNPYIRRST ENGVGHLLQN KFDIMRREDR LSKGEQDLTE RNTIHYGVPI QQIVD EFVF RHRNARGERP LDYFKPFPNF RALRLNRMYR DVEGFSLMKQ RPEFLEWELF TRYRQHHQQR RRLALLHGLE PVANET AQE RDTRRHRLDE ICERTPFDER EMRVNDDEMR VSVETLRSWF GVYMLPSPTV VNAVLGGSAS VNLHLYHLAD EMGTADT RE HVLSSRYLNR LLLLESYQNR VGRGFMNHVV GRAPEPVVPH EQPQEVLRHF SAEERAMYEQ HVKEQTSRQL GEWERAMK R RRWLTDHQQY GHVVSHGLET SVVDLSHTET GAVLTVSTKA YEQEIEAVRM KTNATIKVDG MVYNLLPNSE RRVVPLTVQ LDSGEKIDMT SEDFDRCELE AFPRNLNHAL NYGIANYAYN RGNYVETQDS IWEEQTASGQ EGWSPATHAD GLREGLPVRA RRPIFSSSA EQRIAGGPQR AVIIQYHHQP FFNPEPRLVK VAFQCDGTIM EVPISDVMIW QRRYHGPERT VGDESRRYNP A AMRRYVDV TDPFNEKTSN TEHFLDKYEP KRNADTVADK YRTTKQITEI DKWTRYDSAR ADNYRPLSIS HRRDYIRMGY IP RYTPWEW IAIQEADQPL IAEQIRQDNI GTSYFFSLNR YWRYKASPHG YIRHFENEVR DLLQYVDGVT PWKQAQKIRT YWE VRSHHP MPQFNRPEVA MHRNTVGLLP AHMWETDKKT GKVKSVKDSV RDYQTKTPYP KWVQL

Macromolecule #17: mS50

• Macromolecule: Name: mS50 / type: protein_or_peptide / ID: 17 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 131.71925 KDa

Sequence

String:

MFRVRRLAGF IQCSPCGKMG PLKQQTRRYT PIWKSDPAVD NVAPLRDEDE RRALWAEVGP ISDVGSAVTA WIRFGNDPVL HTAVPTMLG GKFRNQQREK ESLLPNSSSP FAYVEDYMGT NLVFGSPVHA KESAAVWATY FERRYASRLR LSRRTVANYV G LINSPEVF DDESDRPETR WSQDTFFREC AYLSEKFLKE KVSNMQQFEA ALKRASPEAY LAFFDAFQQQ TQTQIPLPSP SV WHYEGER RKQWAEKFIS ISHKAQAFFK DVLSEDVKKY QEVPGKLLQK VKPVLADVGK ILVKRHERWL KGRVWTSLTE EER EAYCMK EVKRQQMQVE DGEFDPMMED DVDDTELEEW QREHDAIMKL MNSPIDGLHF TTLELWLHTM RCEELETEHI YTSA RVRAI QVAARKKLYD TTSYEEVIQA VVESIARGTL DLGAGVLRPH FNEVWCQLNY AKFGSSTITQ HTTTSRRQLL FFHAG SLKD IAATATLYYA TKPLSNSLDY ASPYKYRRSL ITLCSNYGVE TAYTTQRPLL RSAANLARAE DLIHAVVTAA AQPFGE RRR AATRDLHMEF QRLAVPVERV IVANPVSALL ESGADPDEKP VEGEKVNMWP LGAKRVVLYK WSAPNVEKLK AMESDAA SA VSGSSLTAKR LREIQELKRR GFLEVSLWRR VTAQERKQRN EIVEAKKKQV EEVVRTVPSL AHLHQYATSL YSRIEERV A FPTETSTVTE TTNMKEETNK PLEDSEWEFA VLLDDRVLLN KEESVELYLP YRDANGELLA QGEYRALVRA FDLEANPNL HPAYCSVGYS ESFQVFDALP QLIAQFFRVK DATAEAAGVT HIPAADFTPF CAFLRDAGLD VPLRCEFEAG QAVTTDGDVY MDYFLQLLR GEAFHQSHAQ AGLTEAQRAI EPLCRAHWVV HHPGADESEW ATARRSVLDH AMQHEREWWF PNEMLDVKDV V TGSTNGLT PQMYPAAVRY GVELCTVLTA EGKFVDERGS GLSARCVVNG TGAAESVVFD TANCNGTNTT SVEDALRVAH GA LRSAQDR HNTLAAFRLG PLSKQSQVLL FCGVNAYEFG GKYARTYAYA FEKAKKELEA TAASGFMAPS LSHEDTERLS DQP TTSPSV DRFASTTHPE QRKAQFVPRV GPGSTPLEDP AADQKSEWS

Macromolecule #18: mS52

• Macromolecule: Name: mS52 / type: protein_or_peptide / ID: 18 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 84.998234 KDa

Sequence

String:

MRRCSFASTC RYRSADDVPL GLGSAYVWTC RNITSRGQFN PIHNFSYAME RGVRARDVKA FEKLITNPGP LRVAYTPDYL DWLHRCYKA KGTYMDARAV AEKKFNGNIV SSELSAAVNR REGKRGDTRG DTVDNDHHNL PGAPPPGMFL RPAHSFRRLA G ELKRRRAQ SILDEVARAQ GMLDLFERQP HFPAIHIDRC SRFHLVELFK EMVLERSLDS NMIWEKALLY RAILSERKPS YP TSFHYIF TAVEDTVFAP TISTPMEMAG SGGEGHDRSS HPLAAKCPTL EAYYYYVYLV KKYYIDNAVE AHVVLRCHRE PNA ADLLFS NPPPKDDTEI MKAVELLRNA DIQRGVAAAA AVSDPTLPPG GEGSVIGNSD NKKNSEKTSE GSRGRPARPP VLPG AYPPI DMLWRCEENL PLLKVLLFGE FNLIVSENPF VKFPSAHGFL TRPYSTDSSR TLADGMSLAN VMAEKRGHLL PSLPR NTAT SIDARAQDIR RLQQKHHRDD IVSFQKLLRS THAEDSPSAF SSYSDWSYFN PRAVRAEERD RLTRKAVEAL KLYDSA TND IYRHSFEDVQ ACHTQRVTER DRTMPPYLPT LPHFVAIIKK DPHISFLLHI GLPDRNSSEE GSAKHKELEK RIYYLAR AL YHTALEYHNE TVRRVNRQKV NVAASLLDNF VEQEWTTILR DKHDVTDVTK TLNDTQNDKK QLARRLGRYM LFANRSLD D TGFPTDARAD DYTRWMAPPS VGKVSL

Macromolecule #19: mS53

• Macromolecule: Name: mS53 / type: protein_or_peptide / ID: 19 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 75.310867 KDa

Sequence

String:

MSVSGVFSKG RGIGHEATTS ILRYIPRARV PWQPSRFGRE NLTAADMARL WSRGRYRDGP GNYNSGYCTE RTHVLEENTV SIIPRRELE KYMPDITIGP KALVTPVSLM NARNGHRVTH DLLHSYDPHI GRLGKPAVVD HDNITVEDPN RVGLNAATLD C RGRIYRWL RRGPFFQVDN YFRRSVKLNR DGTLPTDFVH EAPLMRKIIR LAHRGHLKAA CEEYRRVTTV PPVEVYRALT AC CVPGAKL ADAVSIFEDG DSKLFYVSRD GEVLHNLMRC AIAARHRARI MWVYNVMRGR FYENVVVRAE VDLIWRYRIA MIA LEYLLD HECAEEAAAI YSYLVEEELL RCDVHVRVGL HMREAIAAGK PITLNNDVMN ATSLVRDATA VAPEVARELQ RRHA QTLQN NAVEAVGAEN VREGSAPWSI LGPLTAIGPT AEDTMVWLQQ HYGDVDVMSI MRWARFRKGK DLMAKDRPQY LARAA AWIE LLSKRNREME EVPLTYMRKS KPLVLDTNSN VRVAWQTPLM RSGGPPRLLA REEGYVFHHS NSSRFVEETY RHPGES LQS RYLALQPLHT EVSAKEDFQR LYYQAQKHHK QQERLLPVST AAIPSRIVHH SVMSALHGVS GKGEPANRSL FTGNKSN DS HSNTGVASGT SACTDGAGSA TPEF

Macromolecule #20: mS54

• Macromolecule: Name: mS54 / type: protein_or_peptide / ID: 20 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 72.046031 KDa

Sequence

String:

MFRRAIPLLS ANIPRSVWDP AQHNPNWSDS YGHDITNRRA WPARKWTVGL EPCTPREWLQ FSHRNLAYAY NGALRACHSL PSMLLLYKE MKQRGVKVDV DTMNVLLTRA ARHEHIQVDD VFLLFDELVA LGARPDLAAA ETLHTVLSHS ASMPEEWREA R RLQLVELY NNLAMEEVER LAPHRADRLL KEQMKRFRGN LQQLGSGLRP TVYCRYLHTT HTAAVLLEEV HNFLWELVPN DH PAMEIPA LQLRVPFVAS VLRRPSVNPG VSLASVSRAE FGDTDVCAVF LAAAERMVDA DFDDQRPVSE RRLFLSLLTM ISY SGVLYT SDLMAQLMEM VKYSNNDETR DSDAQRVLRY ALRGSSAAQD SASRTLWHSV EKVADCRVVG RYIGARNPWN PIRV CFDEQ GVFKAYPIST TTTTREVSPP EGNGAVTQEQ RASCVEGRTL EALNMRWDDV RRLIECTGVL VTPPSERCPQ QQKME VFTG MAVYLRTVAT GRRYEGGEDV LSDGAVATSS CEQRRRGTLF AEGYDFDVWV RLFSLVQEVR HDMEKFMADH TLQCVE PEF ECWEALLVTL RCALDFCVVQ MQGGGARGTE REVVERLFRD VVALREELIE ESRTRFGGRM RVLWLQEA

Macromolecule #21: mS55 (KRIPP8)

• Macromolecule: Name: mS55 (KRIPP8) / type: protein_or_peptide / ID: 21 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 66.67668 KDa

Sequence

String:

MLSQNVAKTT VPSYYMIRTN LPHRKPQNQW EGVYYYSGIT KRQRHLILLH RKREREAHMR SFNISRASVL QRLEQLSGDR KQESLPPHV RLDLAVRLAQ HGLYQQATPI VDELHHQKAL HAGHYALLIN ALACPRLGQR ILHCDAQCDP ALTYKLLGDE N GEERAQEA YRWFDLALTS LAVDCGGRTQ PSHFVPYLPQ GTAAASHITN ALMRTLLTCG YTHVAAIPDS VYDRMGSMGI SP TISTYEL VMLALSLQGN MVEAESILSF LRSHHSEHIT VESFNALLLG HREARQFDCC DAIWQELVDR RWPRASPLTA ELY LRSIMD HANTPTSEPL QSFANINVVE KKKVPLVLAQ MDELGVPRTH LSRVLMDEVE DSLRKFQTYR SRFYEWGRAV KQFD FIEFR RRNGWLYDLH LMKCTTKQVG PLRDFNDPDA VQGAVATAEI PAFFNERPAW ERPPLEETLY VTTNKERYDD VRGGD IYYD DTRGLHDRSP TWMNEVPETR YDRLYGVNHP DIAKIGIRRH LNVEYVNRKE VVERDAALMK KTLSSGRRLR HRVESS RTH RNAGSLSGIS STAGGGSR

Macromolecule #22: mS57

• Macromolecule: Name: mS57 / type: protein_or_peptide / ID: 22 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 45.956293 KDa

Sequence

String:

MFRSTRPRSV GYTPVNPDTS PMVAYSQYHW HYNLPQGMER PHSVNRTFAA PFQSNHSLVN KYRGVWIEFD MHPAFSVALE PQLRKLPRG RTLPKTPAEE VIADYTALAP LVDDEKTRDL WLAKVFQHCA FQRCGGAMEL WERYCHQRFT AEGATAKPPL S LVKSVLFY CNKTDNSGWR ALFDRCLKDG WNYTPLFDTA QWSFMLKSIG RMGDEDGVRA VLEEMLDVQA DLDRVEARSV VI ALNAVTN ADVYEFVKKY LFNFGERKVK FLRTTYSDLR GHGAGKLRIP LKENDNMYYH VCWHSSIRSP RQFSPRQLYF DYT PSTLGS SSHNPNAKID DIVKDKIEKW KAEGLLPEDY VHEDRVYDRS AAFKNVARQE KWKKMPKILK SKRMGYTGDP

Macromolecule #23: mS58

• Macromolecule: Name: mS58 / type: protein_or_peptide / ID: 23 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 35.62391 KDa

Sequence

String:

MSFRYTNNLI GALKHRLLLE SSYREIASRK FIGNCRGVEV VCSGYGTVLA VQLTDKAVWE SFYRKGGRPT VSGGGDVSGD AETGTQGSA TTTGATGSLD LDKLAESIKT ALWDATRKIR SAKEAALHRS LSHNTRMRAS ADLKHWYEED ANTLRPLAFE A LKHEAATP WMQLVQHGKK EEAAALLKEF EQKGDAAEAT PTRVKDDRVK GTRTELSNRE QPPLASTLKA EDSNPATIPI GS VHPLFTP ALVQIEEAGG GSVSNEAVCR AQLWELSRDE QLFWERVELI RKGQVASIGS SHKRGYADEA AFAKDDTEEK VQL RFTQ

Macromolecule #24: mS67

• Macromolecule: Name: mS67 / type: protein_or_peptide / ID: 24 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 30.096051 KDa

Sequence

String:

MLVSYLRLQA DRRTRHHMMV DGQINPREWF NWHPKEYQPW YFDRHKFLYE QRQYAQFHHL LCHQVYLKDM LHLMRYPEPY THIIDCRTS TQKMHRWVPH SLWLPRDEVE YALQLSADEF LDMYGFRKPD KSDDVILLSH NGIYSEQAGW EWKKQFFQHV Y NYRGGTNE LFGESYSDMN VSDVLSPWKG PFPQSGVFVD KWSKRKVLTR TGPFDRQYEM QDFALPDLEL EKARHPEEGP RQ NMPFGLQ

Macromolecule #25: mS69

• Macromolecule: Name: mS69 / type: protein_or_peptide / ID: 25 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 21.589732 KDa

Sequence

String:

MRRVRDGLFL SHPSSALQCS VAVVSTGEFD HPPFQFRQRH TFNTTPLHDA NRFGGRTAYL REIGPVNIKK QGRRFKKDPR TVQFNVDVW CAQQTLRKRW KQRDWEVIEI PFRLVPREQQ RVIPELYTDI PQMTDPARND FSNIRNKVYD REELQGVLFP A AGAMLYPP LQRVDKQAMT LDKYL

Macromolecule #26: mS70

• Macromolecule: Name: mS70 / type: protein_or_peptide / ID: 26 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 21.247398 KDa

Sequence

String:

MLRFTHRALT ATPERFSVLG TTHPKPKRTG FGRNNKMRSK PSDNVAWYDK GPVEWLPRPV RLTYDHLDQL QQWTMRATLD GRTEEFNRI RDLHREWSQH PLMPVLGDVE PKFPLNLFKQ NHRAKKRFLV RWHKANTPAN WLWMPRGPTV VTPLHRTNPT Q YPENWKQM VQRKSGTGTP S

Macromolecule #27: mS71

• Macromolecule: Name: mS71 / type: protein_or_peptide / ID: 27 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 20.093227 KDa

Sequence

String:

MFHRAFVSSS DLTGCTIALS SVCTQKRYWA KPKKRPKVGQ GFHEKAQKWR EEYLLDRHRA LADSLRAYVE FSTSKRVEPW DARFKPFDR IEKDGVYVLM RYMMEEKLQL CNYHHRPVKR LFCNIGLMGP QITTKARWKP YRFATNPAGT SKAERMYQRD K TVYTHGHN D

Macromolecule #28: mS72

• Macromolecule: Name: mS72 / type: protein_or_peptide / ID: 28 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 19.340352 KDa

Sequence

String:

MLRSTLTSLN TFLTSSVATP PISVIRTGPK WWAHPERMVR QKLMYFTLGV DQLPLRRTAV IQRDLQRFHM CKPPPRVGDS TGYKRSRAA QLNTWYRRIQ YQEYHMQHLF TRHVWGLLRV YPGNTTKIQG KADDGYVGYD SVPFHRYNRA PLPFPARELY E RRK

Macromolecule #29: mt-SAF1 (RSM22)

• Macromolecule: Name: mt-SAF1 (RSM22) / type: protein_or_peptide / ID: 29 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 119.799398 KDa

Sequence

String:

MFFRFHSTTL QTCLTSGVAR SSVAVAVPLR AGCTRREMSS GGDGVLEGAM HKPGESGLQA GSSTTIAGKE TWSQFSTKMR YGRRRIRVI DVAAKMSYEY QMLRKMCKRR PAMRQWAVRD DFCDMNPGVV IMSPSMQAAF MKVFRMKEKG LIRQCLRDIV P VIEYRNRE EPARLPTNPA DMIPEGEPDT LNQKKRELFE RREKGENFAD LRLHDKRSQA KLRFRIRQRL LKFQRQLAVA NA IASRSVL YSTNDAIGYF LFRGAAMYAG MHRVFFELSK QLPHFVPKTM LDFGAGTGTA ILVAKEVYDP GSLAYPLYRS LRQ TMQGND SSRTHQLSEL RYDLKRLQRN NEEKKKVRFM AVAALLERGE VDPADLPEDL KREIAEVATA AATAKKDRLV REAH ARYRD VVDGTEWESG DPLGEVRAST EDPEDVIDGE QGDGGDDGEA AKGRPKTWWE KLIDVENETA RTRAARRLRP LQEVT AVEP SPGMMEIGTM VLHDDVPNVT WKRYLLPEDE AIQHDLVVAA YSLSEIATSE NRRRIVQQLW KMTKGVLVFV EFANLN NFN ILMEARDWIL EEKDVGLWDW QPTIVAPCPH EHRCPLRHCK TGVKRKRMRI CSTEAHYRST FVEVWARHMP LKVGIEP IS YLILARNELV PERAERRREQ LKKAEEMKRR ERDVKQQQLH EASLAVKDVV FERLSDEALH RVQSSVPQPL TDIDEVRE A STSATSTSLL KDLKDGATST GEIGHMPTDV PRLVKTGNTR HNRLIFPLQF PPATHKFNRA FVDAGYQRQR AITPAEMLV VRQEVEQLQQ RVMRAAPKYL RVVRDPRCHG KVQADFCTPE GDLVSGRVYR RFYGDRNRVS AHSTMRWQHI GGWKLLKRIR RGSLFPHNV PLYAVTKHAQ IDFPNTLLDT KHSTVEQTAM QYNDPMSLVE MPDDGLTREE LKQKRRLQRD VELQKKVEEK L EDIFGVNA KDWKTDDLGG GRLDARREIS EQQWADAVRR AKIRTVQHTK NALPFAAKKR AAQRALQVRR RNVRLEMSGN RR R

Macromolecule #30: mt-SAF4

• Macromolecule: Name: mt-SAF4 / type: protein_or_peptide / ID: 30 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 91.549312 KDa

Sequence

String:

MRIATSPTRR GKNSSVLPLL RRFSWGNGMC SPYYTEHRRQ STLTSSALSS PANHNQQVVY RSIRDCRERA FHLIQELVSS PIPGCGLSY STNGSFGGPS LTANAVSGVA SLLELYDKAY YFLHLLHRTI LVPRNVVRDT DDFTEFLLRC FRRPSDSLLK G DEELQLSD AAIVDGFVEW METSLMSAGQ FVSFVEVLQL VGSYVRYHKG VRWGARPSEE EWYRCGYRLH PWHDTYCPSS RA EQMPYVH LLQWLMRAKP TKLEEKIDNK EGAHGASSLF STAAKESAMR AWYATEEFSP PSWHTVVGNS NRLGFTALDC GCH SGYMTE LLLKAGAQEV LGVDVSPHHL GNAEATLSEH LRERRSSSHS RKTVQFVRCD ILPDLSDEAR QTKTPPLGPE GSTN SAAAE NRRRLARCHH MPSDSDGLKT ETEVTGPFDL LLFHPPLPLL FPTWPLFHDL YESVDQLAYD AGRRHPHCRL SVLNE FLQR LLGSSNGEEN NSERRRNAHQ QLKHQRRLVA PLIKDNGYVA FILPRNFDTR AILQRMSTSS AGKNCTSCGS DGLAPL VPL SDVVTMTLEG SYTLVLKRSH SLSSLLNRMD YIQKSISAFI RAFVSPQHRS RVEQEVRDFY SNHQAIDLIV MRKIARQ KT NEDNNNKDCS VRAVLQKVSE PIAYEDSFEY EEYIPAGGSP LAHHWTEMTP SFSYLEDEFF GCADSVGTNG PPNGASFL A VGHPLVPVTR QLVGVDRFGA GGRDATQGES GNFDKMFAKE MRRRGGRMRK MALTPLEKQE WYIDEKLVKS EAAKVDLMN ELSRFELKDF D

Macromolecule #31: mt-SAF12 (KRIPP18)

• Macromolecule: Name: mt-SAF12 (KRIPP18) / type: protein_or_peptide / ID: 31 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 65.049215 KDa

Sequence

String:

MLIRRLRRPH STVRGCRISS ASNSDSAGAF SANGTQLPDP PYYLPHSPRF DAERCGTFNK KWLLNLPALK PLVRNSTYLP KKEELWRAP THEALETIIG HLPYHDALRY ITEHSLFLLF PTVLRARDAP LPHVIYEDFM KSCTFASLQN PPEEQFALPS V LLRTLLCM AAYHCTLDAD YFTTCQMLFG RMEQQQQTTP EVLSAWVYCC TASGRVDEAL TYAKYMADCS APFDVTVFSL MQ HPSLNPI EVEDGSVPHS AKGLLLQRRL GNRLHTAYRS DAVAAHGMFV YYALTLSHVR KWEVIRAAAA LGVTLAERTV VLA VEVFAR EKGMRCGPKT VKALTHFLAQ DGTVGHLLYV LLRARKNELL PEFRDLPHTT FSEEEQELVL QCVAQRARHD DSFA VAATL VSSLVREDDP SELLMAFARA ARNHHSADVC GGDGDGSVCA DVPAPVPESP PSNSSEIIEK DRWAVVQASV RSLLL DVNA LDQASRRDAY HKHWKNGNKG VKNKENVLAK TLTLPHDSMH TATIQRKEKE LWELMRSDTP VGVRELAQLN IMEELQ EAK RLERAEMAWV NPDGTF

Macromolecule #32: mt-SAF14

• Macromolecule: Name: mt-SAF14 / type: protein_or_peptide / ID: 32 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 53.32082 KDa

Sequence

String:

MKRIVVPHRW SEMNRVEHPP LMMKQLFQGV CGGLRWLETK SLAQYLAVRA IEEGYPSTPG VRKALHVKRA PLQKRRVVQR STKSATAAC SLPTTAAPAQ LPLTSSGDAL TVTGGLSLQV TKQKRLVSYD VLDCTLGSGY HAGAVLENGG PYTRVVALDC D HDAMHAAR DLVEEFGGDR FRFYCCKMSE AKAMFGERSF DAIMIDGGVS DTQLEDPERG FLLDDEGGHR LDMRFGPQMG VG ALEYLNT VSQHTLVSSL LAYGLLEYGQ AMKMSRAITR RKPFVDSREV LTCIEQAGDE LPEGGWRSQG SRRKSPMSWK FLT SLRCII NNEMYELRQG IENALLMLRD DGRLVVFSRL PWEERLVRGT VDDHPHALLS YVEDISIDDV QIYGFTRHAK MWVI TRAAS SAYALKNTTT LTEEKFRESS VRWLTGMYAG QTHGFPANNF TFENFERKEW VTLRRNGKPP PVDVGLDDK

Macromolecule #33: mt-SAF15

• Macromolecule: Name: mt-SAF15 / type: protein_or_peptide / ID: 33 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 53.273867 KDa

Sequence

String:

MRWSAVLCKV SKVPTRSVIP ADALRYHPRY SVPRKMVLSN TFNVVGENNR YTSLKLILEK LSGHVGRRQY KMLCNLEKHY DKLKDEGID WHELKWLSRE ELIVLFDKVL HLTRTERAAL LPAIEAKVCG VLRQTDSRHS TVVCMNRGNA NHGWRCGRNG H TNDVYFEG KAKANTAALQ LCRRSSVRSV ERSGVLVEVR SEPDFSVVGR FDHSLSPRVW STPENPTFQV TTIGYEFRVH QE DPRVIPQ IVEAAEEWEL HANITKQVIW EMLEMYAVER DRQPLDLKPG EMGDPDIPSR HATAFNVQVV PPLSEGDEAI QVV EQRIVR ADGSEVPWFQ EPPPQLFSGG IPVILPFAPS IIVKSTFRQV TRSSAQDVTR QLLQPVVDVT CFLHPNVCFW WNAE DEQRC LGHIVDYAKR IPFALPFNLY FRVNLSKDLR GVQNYTEELG KRMSMKAHYF NLRSYGVR

Macromolecule #34: mt-SAF16

• Macromolecule: Name: mt-SAF16 / type: protein_or_peptide / ID: 34 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 50.911414 KDa

Sequence

String:

MLVCCRSSLS LLARATMPLC CSRRFLTHQN NIDDISGPVD TNSNSVSDGR LHCSTGEGGK ASTCERVSLR TIAESLGAAA AAELRAEVE RDTRDGVAAI PPLPPLGWRV RHPSGSNYFV MTRTLKNGVQ SAELNNRRYR SVHDIFLQSL QKGGHKYAQK G KGSEGRQK DAKEEEEPPS QEDGKSSPKV TVGYDREGRG HRATLQRMDE LHDSPKLSRA DVHLTVFAPF RVYDPSLHDP TV DICEWSS FDLVVQKTVP DNMVANKLLQ PLSCTPQDGA LSMYVCLASV NSEMRIRSIQ LLSMKEAQAL VEHACFGNGE PLF LELLRR RGRRRPLVER RFDDPRLRYE EVAQPQQVAD EAAVACSSSC YGPYYPAFEM LMDSCGSAGE YSRALCYGGP YVSE LSREL CDALLDYIKG DLGVSDQLCE YVCQMQFFLE QEEYMTWLGQ VQHVANAVSR TA

Macromolecule #35: mt-SAF17

• Macromolecule: Name: mt-SAF17 / type: protein_or_peptide / ID: 35 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 50.14759 KDa

Sequence

String:

MQRTLRSAAR RKWGQKTWSP TATNGGAAPA NGVSAQEALQ IAYRPMPPSQ TVEYEEDFGH NLMIHREYIS KRCRDRVSFE LSALSYSNL ELRRGQEHLA GIMNRERRGV SVGASGAPDD QVQMQTDVDA NSREVLSARY LFNERRLQFC DRFQNFFQSK L ENSAASDS NGHEKQHLFS LMEACAVIFG CETEAARETY YRMFLGLDSE TLLEEDEALR NRIADAKLVQ RVLENNKGRQ EV TQSPKLQ QQQDQGKPLH AVSSGTSLLN DCEEERFISS IPELSLFEDE TEARANGFVE GEDDVKANNG DLTAGSFSSP ASS VNLPEE FEEYAPLYKA YITHAVGKGP VASYDISTLG STGLTAERRR WRTLMEKIVR EDYHTMTEVE QMDAIVLNEQ LHTV KFFDL KIGDAIRDIL QLLQRETGVG SSVNRDTPVG ISPNNPERRV

Macromolecule #36: mt-SAF19

• Macromolecule: Name: mt-SAF19 / type: protein_or_peptide / ID: 36 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei / Strain: Lister strain 427
• Molecular weight: Theoretical: 41.236418 KDa

Sequence

String:

MHSSLIILRH AYFSALHPAR RVVPGSLLPV RTQFYTRHFT STAGPTCGDG GETYKSEPTK VGASVEGTNS GNGVTDSPSL FSSSAPTVR RRALPPSDFP ENALLKCIEK EIEDEALRLD KEECPPPPPT GWEMYHAPGT SVFYGRRWWL PATASAETRA T PERHTIRV QLTKRDPSLD PECDVRGEHF PFSFFVQRAP SKGEAVRRDG TFRMGDSAAA GDVKGRTEGK EEEEEEEELG LY DQSIEVR ADFVDGELLV DNVVFHGTFK TGSSCSKRSG NTSPEAAAAT AAGQHDNTTG GRGKVEEVRY NNIFNGYPGP NLD EAEEEV LDGLQAWLAE RCVDDQFGEF VGQYSVWVEQ QEYEMWLKRL RDFVAA

Macromolecule #37: mt-SAF20

• Macromolecule: Name: mt-SAF20 / type: protein_or_peptide / ID: 37 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 40.293855 KDa

Sequence

String:

MRHFRLNRCS YTPNLTSLTN QVNRSERLRK WGSAGVPPGV PRIPRLEAKG IAILHESPKV ILAGRSRCNN FDSNQYMLIN KATKRCLLV DASDDWPDDW AAFIGASDLT LTHVFLTHCH IDNIINLNAF LTICGSRQKQ RVQVDSQDND NREDDNGSDE I GVMWCPAE ECWVQNFKRS CERYGRFEEM HQVLPMMCRS LYTPQHLVDP VIAGFSRRNA RHLRRNDVLL SAATNRATSF ID FGNGVLL YYIFSPGHSP GHMMLHIPTE RILFSGDLLF FNKVGRVDLP WATGVRLAES LRLLEALPDN TVVVPGHGRM TTL GRERRE NKALQQCYQR QEIGKQEVSV GFNEGYL

Macromolecule #38: mt-SAF25

• Macromolecule: Name: mt-SAF25 / type: protein_or_peptide / ID: 38 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 29.470537 KDa

Sequence

String:

MMKRTILQRC IQNKSLEIAR ISRSDINSRA HLPFNFDVCY ELGSREFTLF SSVGSTSVLV FCNVSSRRLR SVKGGQGETE FPPKRLNVK RRRQSGSADR SPVVFSAFVS MPTSGLTIEA LCCSSLGLLV VDGVSFHQGP LTESMIPQDA HPGPEGHYQG P LLNQRSLA EMVVNTRGCI TSVDPFRQQE SFFDGHVNPW NARSLRFGHV PVHTAKPGFS DALCHFLEVF GVNDELAFFV ED FAHLVHR EEETAWTNVL KTMMGGR

Macromolecule #39: mt-SAF34

• Macromolecule: Name: mt-SAF34 / type: protein_or_peptide / ID: 39 ; Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei / Strain: Lister strain 427
• Molecular weight: Theoretical: 14.862911 KDa

Sequence

String:

MSRSTVFGPG SLYSFTKFGS FNRSPTNCTL NKRMKDIFRL ENQKHIRNDF DRERRYRMCT KCGITTVTIN FNNVPSARVG LWGRCADDK DYTHHRMVDI TQREYEVLRE SPVEKRLNWW RYER

Macromolecule #40: UNK-a

• Macromolecule: Name: UNK-a / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 5.975357 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)

Macromolecule #41: UNK-b

• Macromolecule: Name: UNK-b / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 3.592419 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)

Macromolecule #42: UNK-c

• Macromolecule: Name: UNK-c / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 1.039273 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)

Macromolecule #43: UNK-d

• Macromolecule: Name: UNK-d / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 5.039203 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)

Macromolecule #44: UNK-e

• Macromolecule: Name: UNK-e / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 2.486056 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #45: UNK-f

• Macromolecule: Name: UNK-f / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 783.958 Da

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #46: UNK-g

• Macromolecule: Name: UNK-g / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 14.230525 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #47: UNK-h

• Macromolecule: Name: UNK-h / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 21.719672 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #48: UNK-i

• Macromolecule: Name: UNK-i / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 2.74137 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

Macromolecule #49: UNK-j

• Macromolecule: Name: UNK-j / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 1.635006 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

Macromolecule #50: UNK-k

• Macromolecule: Name: UNK-k / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 2.315846 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #51: UNK-l

• Macromolecule: Name: UNK-l / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 1.209482 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)

Macromolecule #52: UNK-m

• Macromolecule: Name: UNK-m / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 954.168 Da

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)

Macromolecule #53: UNK-x

• Macromolecule: Name: UNK-x / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 9.209344 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)

Macromolecule #7: 9S rRNA

• Macromolecule: Name: 9S rRNA / type: rna / ID: 7 ; Details: 9S ribosomal RNA from Trypanosoma brucei. GenBank: X02547.1 and S66467.1; Number of copies: 1
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
• Molecular weight: Theoretical: 256.380734 KDa

Sequence

String:

UAAAUUAUGG UCAAUUGUUA GUAUUCAUAU UAAUUUUUUU AAAUGUUUUA UCAUUUUAUA AAGGUUUAUU UUUGAAAGAU UUUUUGUAU AAAAUUUUAG GAAUAGUUAA UAAUAAUUUA UAAUUUUGAU UAGAUUGUUU UGUUAAUGCU AUUAGAUGGG U GUGGAAAA AUAAAAAAAA UAAUUAAUAU AUAUCAAUAA UAAAUUAAAU UAAUCUAUUA GUCAGAAAUG GAUGCCAGCC GU UGCGGUA AUUUCUAUGC UUUUAAAUAU UAUACAAUUA UCAUAUUAAA UUGUUAAGUG UUGAUUUAAC CAAUAAAAAU AUA AAUAAU UUUUAUUUGU UUUUAAACAC CAUUAGGUAU AUGCAAAUAU AAAAUUAUAG UAAUUAUAAA UUAUAUUAUA UUAU AUUUA UUCAUAUAAU UAAUAGGAUA AUAUUUGUAG UUUUUGAUAC CAUGAUAAGG AUUAUAAAUU GAAAGUGUUA AUAUC AUAA UCAAAAUUUA UUAUUUAUAU UAAAUAUGUA UGUGUAGAUA AAAUAAGAAA UUAAAAAGGU AUUGUUGCCC ACCAAU UUU UAAAUUAUAU UAUAUUAUAU UUAUUCAUAU AAUUAAUAGG AUAAUAUUUG UAGUUUUUGA UACCAUGAUA AGGAUUA UA AAUUGAAAGU GUUAAUAUCA UAAUCAAAAU UUAUUAUUUA UAUUAAAUAU GUAUGUGUAG AUAAAAUAAG AAAUUAAA A AGGUAUUGUU GCCCACCAAU UUUUAUAAUA AAAAUAACGU GCAGUAAUUA AUAUAUUUAU AAAAAUAUAU UUUUUUUUU UA

Macromolecule #54: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 54 / Number of copies: 1 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

Macromolecule #55: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 55 / Number of copies: 1 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #56: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 56 / Number of copies: 5 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #57: S-ADENOSYL-L-HOMOCYSTEINE

• Macromolecule: Name: S-ADENOSYL-L-HOMOCYSTEINE / type: ligand / ID: 57 / Number of copies: 2 / Formula: SAH
• Molecular weight: Theoretical: 384.411 Da

Chemical component information

ChemComp-SAH:
S-ADENOSYL-L-HOMOCYSTEINE / S-Adenosyl-L-homocysteine

Macromolecule #58: water

• Macromolecule: Name: water / type: ligand / ID: 58 / Number of copies: 3 / Formula: HOH
• Molecular weight: Theoretical: 18.015 Da

Chemical component information

ChemComp-HOH:
WATER / Water

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.4
• Grid: Model: Quantifoil R2/2 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Pretreatment - Type: GLOW DISCHARGE
• Vitrification: Cryogen name: ETHANE-PROPANE / Chamber humidity: 90 % / Chamber temperature: 278 K / Instrument: FEI VITROBOT MARK IV
• Details: Head domain of the mitochondrial ribosomal small subunit assemblosome of Trypanosoma brucei brucei Lister strain 427

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Calibrated magnification: 100719 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal magnification: 59000
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Image recording: Film or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Average electron dose: 40.0 e/Ų
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• CTF correction: Software - Name: Gctf / Details: On the fly in RELION
• Startup model: Type of model: OTHER; Details: Startup model calculated from a small negative stain data set using EMD-0230 (T.brucei mitoribosomal small subunit) as an initial reference for classification of mitochondrial small subunit-like classes
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION
• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 161661

Atomic model buiding 1

• Details: Starting models of ribosomal proteins of the T. brucei mitochondrial ribosomal small subunit were taken from PDB 6HIW. Mitchondrial ribosomal small subunit assembly factors were built de novo.
• Refinement: Protocol: OTHER

Output model

PDB-6sg9:
Head domain of the mt-SSU assemblosome from Trypanosoma brucei