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-Structure paper
Title | Mitoribosomal small subunit biogenesis in trypanosomes involves an extensive assembly machinery. |
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Journal, issue, pages | Science, Vol. 365, Issue 6458, Page 1144-1149, Year 2019 |
Publish date | Sep 13, 2019 |
Authors | Martin Saurer / David J F Ramrath / Moritz Niemann / Salvatore Calderaro / Céline Prange / Simone Mattei / Alain Scaiola / Alexander Leitner / Philipp Bieri / Elke K Horn / Marc Leibundgut / Daniel Boehringer / André Schneider / Nenad Ban / |
PubMed Abstract | Mitochondrial ribosomes (mitoribosomes) are large ribonucleoprotein complexes that synthesize proteins encoded by the mitochondrial genome. An extensive cellular machinery responsible for ribosome ...Mitochondrial ribosomes (mitoribosomes) are large ribonucleoprotein complexes that synthesize proteins encoded by the mitochondrial genome. An extensive cellular machinery responsible for ribosome assembly has been described only for eukaryotic cytosolic ribosomes. Here we report that the assembly of the small mitoribosomal subunit in involves a large number of factors and proceeds through the formation of assembly intermediates, which we analyzed by using cryo-electron microscopy. One of them is a 4-megadalton complex, referred to as the small subunit assemblosome, in which we identified 34 factors that interact with immature ribosomal RNA (rRNA) and recognize its functionally important regions. The assembly proceeds through large-scale conformational changes in rRNA coupled with successive incorporation of mitoribosomal proteins, providing an example for the complexity of the ribosomal assembly process in mitochondria. |
External links | Science / PubMed:31515389 |
Methods | EM (single particle) |
Resolution | 3.1 - 5.9 Å |
Structure data | EMDB-10175, PDB-6sg9: EMDB-10176: EMDB-10177, PDB-6sga: EMDB-10178: EMDB-10179: EMDB-10180, PDB-6sgb: |
Chemicals | ChemComp-GTP: ChemComp-MG: ChemComp-ZN: ChemComp-SAH: ChemComp-HOH: ChemComp-PO4: ChemComp-PM8: |
Source |
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Keywords | RIBOSOME / mitochondrial ribosome / assembly intermediate / translation |