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データを開く
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基本情報
登録情報 | データベース: EMDB / ID: EMD-0492 | ||||||||||||||||||||||||
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タイトル | hTRiC-hPFD Class1 (No PFD) | ||||||||||||||||||||||||
![]() | hTRiC-hPFD Class1 (No PFD) | ||||||||||||||||||||||||
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![]() | TRiC/CCT / PFD / CryoEM / Molecular Chaperone / Protein folding / CHAPERONE | ||||||||||||||||||||||||
機能・相同性 | ![]() zona pellucida receptor complex / scaRNA localization to Cajal body / chaperone mediated protein folding independent of cofactor / positive regulation of establishment of protein localization to telomere / positive regulation of protein localization to Cajal body / tubulin complex assembly / BBSome-mediated cargo-targeting to cilium / binding of sperm to zona pellucida / Folding of actin by CCT/TriC / Formation of tubulin folding intermediates by CCT/TriC ...zona pellucida receptor complex / scaRNA localization to Cajal body / chaperone mediated protein folding independent of cofactor / positive regulation of establishment of protein localization to telomere / positive regulation of protein localization to Cajal body / tubulin complex assembly / BBSome-mediated cargo-targeting to cilium / binding of sperm to zona pellucida / Folding of actin by CCT/TriC / Formation of tubulin folding intermediates by CCT/TriC / positive regulation of telomerase RNA localization to Cajal body / chaperonin-containing T-complex / Prefoldin mediated transfer of substrate to CCT/TriC / RHOBTB1 GTPase cycle / intermediate filament cytoskeleton / WD40-repeat domain binding / pericentriolar material / beta-tubulin binding / Association of TriC/CCT with target proteins during biosynthesis / : / chaperone-mediated protein complex assembly / RHOBTB2 GTPase cycle / heterochromatin / chaperone-mediated protein folding / protein folding chaperone / positive regulation of telomere maintenance via telomerase / Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation / acrosomal vesicle / cell projection / mRNA 3'-UTR binding / ATP-dependent protein folding chaperone / response to virus / cilium / mRNA 5'-UTR binding / Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding / G-protein beta-subunit binding / azurophil granule lumen / unfolded protein binding / melanosome / protein folding / cell body / secretory granule lumen / ficolin-1-rich granule lumen / microtubule / cytoskeleton / protein stabilization / cadherin binding / centrosome / ubiquitin protein ligase binding / Neutrophil degranulation / Golgi apparatus / ATP hydrolysis activity / RNA binding / extracellular exosome / extracellular region / nucleoplasm / ATP binding / cytoplasm / cytosol 類似検索 - 分子機能 | ||||||||||||||||||||||||
生物種 | ![]() | ||||||||||||||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 7.7 Å | ||||||||||||||||||||||||
![]() | Gestaut DR / Roh SH | ||||||||||||||||||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: The Chaperonin TRiC/CCT Associates with Prefoldin through a Conserved Electrostatic Interface Essential for Cellular Proteostasis. 著者: Daniel Gestaut / Soung Hun Roh / Boxue Ma / Grigore Pintilie / Lukasz A Joachimiak / Alexander Leitner / Thomas Walzthoeni / Ruedi Aebersold / Wah Chiu / Judith Frydman / ![]() ![]() ![]() ![]() 要旨: Maintaining proteostasis in eukaryotic protein folding involves cooperation of distinct chaperone systems. To understand how the essential ring-shaped chaperonin TRiC/CCT cooperates with the ...Maintaining proteostasis in eukaryotic protein folding involves cooperation of distinct chaperone systems. To understand how the essential ring-shaped chaperonin TRiC/CCT cooperates with the chaperone prefoldin/GIMc (PFD), we integrate cryoelectron microscopy (cryo-EM), crosslinking-mass-spectrometry and biochemical and cellular approaches to elucidate the structural and functional interplay between TRiC/CCT and PFD. We find these hetero-oligomeric chaperones associate in a defined architecture, through a conserved interface of electrostatic contacts that serves as a pivot point for a TRiC-PFD conformational cycle. PFD alternates between an open "latched" conformation and a closed "engaged" conformation that aligns the PFD-TRiC substrate binding chambers. PFD can act after TRiC bound its substrates to enhance the rate and yield of the folding reaction, suppressing non-productive reaction cycles. Disrupting the TRiC-PFD interaction in vivo is strongly deleterious, leading to accumulation of amyloid aggregates. The supra-chaperone assembly formed by PFD and TRiC is essential to prevent toxic conformations and ensure effective cellular proteostasis. | ||||||||||||||||||||||||
履歴 |
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構造の表示
ムービー |
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構造ビューア | EMマップ: ![]() ![]() ![]() |
添付画像 |
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ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | 49.1 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 29.5 KB 29.5 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 8.6 KB | 表示 | ![]() |
画像 | ![]() | 55.3 KB | ||
Filedesc metadata | ![]() | 8.6 KB | ||
その他 | ![]() ![]() ![]() | 40 MB 40.7 MB 40.7 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-検証レポート
文書・要旨 | ![]() | 829.4 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 828.9 KB | 表示 | |
XML形式データ | ![]() | 14.2 KB | 表示 | |
CIF形式データ | ![]() | 20.2 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 6nraMC ![]() 0490C ![]() 0491C ![]() 0493C ![]() 0494C ![]() 0495C ![]() 0496C ![]() 6nr8C ![]() 6nr9C ![]() 6nrbC ![]() 6nrcC ![]() 6nrdC C: 同じ文献を引用 ( M: このマップから作成された原子モデル |
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類似構造データ |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | hTRiC-hPFD Class1 (No PFD) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.7 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
-追加マップ: hTRiC-hPFD Class1 (No PFD), unfiltered sum map
ファイル | emd_0492_additional.map | ||||||||||||
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注釈 | hTRiC-hPFD Class1 (No PFD), unfiltered sum map | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: hTRiC-hPFD Class1 (No PFD), half map 1
ファイル | emd_0492_half_map_1.map | ||||||||||||
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注釈 | hTRiC-hPFD Class1 (No PFD), half map 1 | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: hTRiC-hPFD Class1 (No PFD), half map 2
ファイル | emd_0492_half_map_2.map | ||||||||||||
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注釈 | hTRiC-hPFD Class1 (No PFD), half map 2 | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
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試料の構成要素
-全体 : hTRiC
全体 | 名称: hTRiC |
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要素 |
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-超分子 #1: hTRiC
超分子 | 名称: hTRiC / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: all |
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由来(天然) | 生物種: ![]() |
-分子 #1: T-complex protein 1 subunit alpha
分子 | 名称: T-complex protein 1 subunit alpha / タイプ: protein_or_peptide / ID: 1 / コピー数: 2 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 58.061055 KDa |
組換発現 | 生物種: Insect cell expression vector pTIE1 (その他) |
配列 | 文字列: MEGPLSVFGD RSTGETIRSQ NVMAAASIAN IVKSSLGPVG LDKMLVDDIG DVTITNDGAT ILKLLEVEHP AAKVLCELAD LQDKEVGDG TTSVVIIAAE LLKNADELVK QKIHPTSVIS GYRLACKEAV RYINENLIVN TDELGRDCLI NAAKTSMSSK I IGINGDFF ...文字列: MEGPLSVFGD RSTGETIRSQ NVMAAASIAN IVKSSLGPVG LDKMLVDDIG DVTITNDGAT ILKLLEVEHP AAKVLCELAD LQDKEVGDG TTSVVIIAAE LLKNADELVK QKIHPTSVIS GYRLACKEAV RYINENLIVN TDELGRDCLI NAAKTSMSSK I IGINGDFF ANMVVDAVLA IKYTDIRGQP RYPVNSVNIL KAHGRSQMES MLISGYALNC VVGSQGMPKR IVNAKIACLD FS LQKTKMK LGVQVVITDP EKLDQIRQRE SDITKERIQK ILATGANVIL TTGGIDDMCL KYFVEAGAMA VRRVLKRDLK RIA KASGAT ILSTLANLEG EETFEAAMLG QAEEVVQERI CDDELILIKN TKARTSASII LRGANDFMCD EMERSLHDAL CVVK RVLES KSVVPGGGAV EAALSIYLEN YATSMGSREQ LAIAEFARSL LVIPNTLAVN AAQDSTDLVA KLRAFHNEAQ VNPER KNLK WIGLDLSNGK PRDNKQAGVF EPTIVKVKSL KFATEAAITI LRIDDLIKLH UniProtKB: T-complex protein 1 subunit alpha |
-分子 #2: T-complex protein 1 subunit beta
分子 | 名称: T-complex protein 1 subunit beta / タイプ: protein_or_peptide / ID: 2 / コピー数: 2 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 54.736742 KDa |
組換発現 | 生物種: Insect cell expression vector pTIE1 (その他) |
配列 | 文字列: ADEERAETAR LTSFIGAIAI GDLVKSTLGP KGMDKILLSS GRDASLMVTN DGATILKNIG VDNPAAKVLV DMSRVQDDEV GDGTTSVTV LAAELLREAE SLIAKKIHPQ TIIAGWREAT KAAREALLSS AVDHGSDEVK FRQDLMNIAG TTLSSKLLTH H KDHFTKLA ...文字列: ADEERAETAR LTSFIGAIAI GDLVKSTLGP KGMDKILLSS GRDASLMVTN DGATILKNIG VDNPAAKVLV DMSRVQDDEV GDGTTSVTV LAAELLREAE SLIAKKIHPQ TIIAGWREAT KAAREALLSS AVDHGSDEVK FRQDLMNIAG TTLSSKLLTH H KDHFTKLA VEAVLRLKGS GNLEAIHIIK KLGGSLADSY LDEGFLLDKK IGVNQPKRIE NAKILIANTG MDTDKIKIFG SR VRVDSTA KVAEIEHAEK EKMKEKVERI LKHGINCFIN RQLIYNYPEQ LFGAAGVMAI EHADFAGVER LALVTGGEIA STF DHPELV KLGSCKLIEE VMIGEDKLIH FSGVALGEAC TIVLRGATQQ ILDEAERSLH DALCVLAQTV KDSRTVYGGG CSEM LMAHA VTQLANRTPG KEAVAMESYA KALRMLPTII ADNAGYDSAD LVAQLRAAHS EGNTTAGLDM REGTIGDMAI LGITE SFQV KRQVLLSAAE AAEVILRVDN IIKAA UniProtKB: T-complex protein 1 subunit beta |
-分子 #3: T-complex protein 1 subunit gamma
分子 | 名称: T-complex protein 1 subunit gamma / タイプ: protein_or_peptide / ID: 3 / コピー数: 2 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 57.165945 KDa |
組換発現 | 生物種: Insect cell expression vector pTIE1 (その他) |
配列 | 文字列: NTKRESGRKV QSGNINAAKT IADIIRTCLG PKSMMKMLLD PMGGIVMTND GNAILREIQV QHPAAKSMIE ISRTQDEEVG DGTTSVIIL AGEMLSVAEH FLEQQMHPTV VISAYRKALD DMISTLKKIS IPVDISDSDM MLNIINSSIT TKAISRWSSL A CNIALDAV ...文字列: NTKRESGRKV QSGNINAAKT IADIIRTCLG PKSMMKMLLD PMGGIVMTND GNAILREIQV QHPAAKSMIE ISRTQDEEVG DGTTSVIIL AGEMLSVAEH FLEQQMHPTV VISAYRKALD DMISTLKKIS IPVDISDSDM MLNIINSSIT TKAISRWSSL A CNIALDAV KMVQFEENGR KEIDIKKYAR VEKIPGGIIE DSCVLRGVMI NKDVTHPRMR RYIKNPRIVL LDSSLEYKKG ES QTDIEIT REEDFTRILQ MEEEYIQQLC EDIIQLKPDV VITEKGISDL AQHYLMRANI TAIRRVRKTD NNRIARACGA RIV SRPEEL REDDVGTGAG LLEIKKIGDE YFTFITDCKD PKACTILLRG ASKEILSEVE RNLQDAMQVC RNVLLDPQLV PGGG ASEMA VAHALTEKSK AMTGVEQWPY RAVAQALEVI PRTLIQNCGA STIRLLTSLR AKHTQENCET WGVNGETGTL VDMKE LGIW EPLAVKLQTY KTAVETAVLL LRIDDIVSG UniProtKB: T-complex protein 1 subunit gamma |
-分子 #4: T-complex protein 1 subunit delta
分子 | 名称: T-complex protein 1 subunit delta / タイプ: protein_or_peptide / ID: 4 / コピー数: 2 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 55.6365 KDa |
組換発現 | 生物種: Insect cell expression vector pTIE1 (その他) |
配列 | 文字列: DRDKPAQIRF SNISAAKAVA DAIRTSLGPK GMDKMIQDGK GDVTITNDGA TILKQMQVLH PAARMLVELS KAQDIEAGDG TTSVVIIAG SLLDSCTKLL QKGIHPTIIS ESFQKALEKG IEILTDMSRP VELSDRETLL NSATTSLNSK VVSQYSSLLS P MSVNAVMK ...文字列: DRDKPAQIRF SNISAAKAVA DAIRTSLGPK GMDKMIQDGK GDVTITNDGA TILKQMQVLH PAARMLVELS KAQDIEAGDG TTSVVIIAG SLLDSCTKLL QKGIHPTIIS ESFQKALEKG IEILTDMSRP VELSDRETLL NSATTSLNSK VVSQYSSLLS P MSVNAVMK VIDPATATSV DLRDIKIVKK LGGTIDDCEL VEGLVLTQKV SNSGITRVEK AKIGLIQFCL SAPKTDMDNQ IV VSDYAQM DRVLREERAY ILNLVKQIKK TGCNVLLIQK SILRDALSDL ALHFLNKMKI MVIKDIERED IEFICKTIGT KPV AHIDQF TADMLGSAEL AEEVNLNGSG KLLKITGCAS PGKTVTIVVR GSNKLVIEEA ERSIHDALCV IRCLVKKRAL IAGG GAPEI ELALRLTEYS RTLSGMESYC VRAFADAMEV IPSTLAENAG LNPISTVTEL RNRHAQGEKT AGINVRKGGI SNILE ELVV QPLLVSVSAL TLATETVRSI LKIDDVVNTR UniProtKB: T-complex protein 1 subunit delta |
-分子 #5: T-complex protein 1 subunit epsilon
分子 | 名称: T-complex protein 1 subunit epsilon / タイプ: protein_or_peptide / ID: 5 / コピー数: 2 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 56.989797 KDa |
組換発現 | 生物種: Insect cell expression vector pTIE1 (その他) |
配列 | 文字列: KSRLMGLEAL KSHIMAAKAV ANTMRTSLGP NGLDKMMVDK DGDVTVTNDG ATILSMMDVD HQIAKLMVEL SKSQDDEIGD GTTGVVVLA GALLEEAEQL LDRGIHPIRI ADGYEQAARV AIEHLDKISD SVLVDIKDTE PLIQTAKTTL GSKVVNSCHR Q MAEIAVNA ...文字列: KSRLMGLEAL KSHIMAAKAV ANTMRTSLGP NGLDKMMVDK DGDVTVTNDG ATILSMMDVD HQIAKLMVEL SKSQDDEIGD GTTGVVVLA GALLEEAEQL LDRGIHPIRI ADGYEQAARV AIEHLDKISD SVLVDIKDTE PLIQTAKTTL GSKVVNSCHR Q MAEIAVNA VLTVADMERR DVDFELIKVE GKVGGRLEDT KLIKGVIVDK DFSHPQMPKK VEDAKIAILT CPFEPPKPKT KH KLDVTSV EDYKALQKYE KEKFEEMIQQ IKETGANLAI CQWGFDDEAN HLLLQNNLPA VRWVGGPEIE LIAIATGGRI VPR FSELTA EKLGFAGLVQ EISFGTTKDK MLVIEQCKNS RAVTIFIRGG NKMIIEEAKR SLHDALCVIR NLIRDNRVVY GGGA AEISC ALAVSQEADK CPTLEQYAMR AFADALEVIP MALSENSGMN PIQTMTEVRA RQVKEMNPAL GIDCLHKGTN DMKQQ HVIE TLIGKKQQIS LATQMVRMIL KIDDIRKPGE SEE UniProtKB: T-complex protein 1 subunit epsilon |
-分子 #6: T-complex protein 1 subunit zeta
分子 | 名称: T-complex protein 1 subunit zeta / タイプ: protein_or_peptide / ID: 6 / コピー数: 2 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 56.445008 KDa |
組換発現 | 生物種: Insect cell expression vector pTIE1 (その他) |
配列 | 文字列: AEVARAQAAL AVNISAARGL QDVLRTNLGP KGTMKMLVSG AGDIKLTKDG NVLLHEMQIQ HPTASLIAKV ATAQDDITGD GTTSNVLII GELLKQADLY ISEGLHPRII TEGFEAAKEK ALQFLEEVKV SREMDRETLI DVARTSLRTK VHAELADVLT E AVVDSILA ...文字列: AEVARAQAAL AVNISAARGL QDVLRTNLGP KGTMKMLVSG AGDIKLTKDG NVLLHEMQIQ HPTASLIAKV ATAQDDITGD GTTSNVLII GELLKQADLY ISEGLHPRII TEGFEAAKEK ALQFLEEVKV SREMDRETLI DVARTSLRTK VHAELADVLT E AVVDSILA IKKQDEPIDL FMIEIMEMKH KSETDTSLIR GLVLDHGARH PDMKKRVEDA YILTCNVSLE YEKTEVNSGF FY KSAEERE KLVKAERKFI EDRVKKIIEL KRKVCGDSDK GFVVINQKGI DPFSLDALSK EGIVALRRAK RRNMERLTLA CGG VALNSF DDLSPDCLGH AGLVYEYTLG EEKFTFIEKC NNPRSVTLLI KGPNKHTLTQ IKDAVRDGLR AVKNAIDDGC VVPG AGAVE VAMAEALIKH KPSVKGRAQL GVQAFADALL IIPKVLAQNS GFDLQETLVK IQAEHSESGQ LVGVDLNTGE PMVAA EVGV WDNYCVKKQL LHSCTVIATN ILLVDEIMRA G UniProtKB: T-complex protein 1 subunit zeta |
-分子 #7: T-complex protein 1 subunit eta
分子 | 名称: T-complex protein 1 subunit eta / タイプ: protein_or_peptide / ID: 7 / コピー数: 2 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 56.369867 KDa |
組換発現 | 生物種: Insect cell expression vector pTIE1 (その他) |
配列 | 文字列: GTDSSQGIPQ LVSNISACQV IAEAVRTTLG PRGMDKLIVD GRGKATISND GATILKLLDV VHPAAKTLVD IAKSQDAEVG DGTTSVTLL AAEFLKQVKP YVEEGLHPQI IIRAFRTATQ LAVNKIKEIA VTVKKADKVE QRKLLEKCAM TALSSKLISQ Q KAFFAKMV ...文字列: GTDSSQGIPQ LVSNISACQV IAEAVRTTLG PRGMDKLIVD GRGKATISND GATILKLLDV VHPAAKTLVD IAKSQDAEVG DGTTSVTLL AAEFLKQVKP YVEEGLHPQI IIRAFRTATQ LAVNKIKEIA VTVKKADKVE QRKLLEKCAM TALSSKLISQ Q KAFFAKMV VDAVMMLDDL LQLKMIGIKK VQGGALEDSQ LVAGVAFKKT FSYAGFEMQP KKYHNPKIAL LNVELELKAE KD NAEIRVH TVEDYQAIVD AEWNILYDKL EKIHHSGAKV VLSKLPIGDV ATQYFADRDM FCAGRVPEED LKRTMMACGG SIQ TSVNAL SADVLGRCQV FEETQIGGER YNFFTGCPKA KTCTFILRGG AEQFMEETER SLHDAIMIVR RAIKNDSVVA GGGA IEMEL SKYLRDYSRT IPGKQQLLIG AYAKALEIIP RQLCDNAGFD ATNILNKLRA RHAQGGTWYG VDINNEDIAD NFEAF VWEP AMVRINALTA ASEAACLIVS VDETIKNPRS UniProtKB: T-complex protein 1 subunit eta |
-分子 #8: T-complex protein 1 subunit theta
分子 | 名称: T-complex protein 1 subunit theta / タイプ: protein_or_peptide / ID: 8 / コピー数: 2 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 56.102406 KDa |
組換発現 | 生物種: Insect cell expression vector pTIE1 (その他) |
配列 | 文字列: MLKEGAKHFS GLEEAVYRNI QACKELAQTT RTAYGPNGMN KMVINHLEKL FVTNDAATIL RELEVQHPAA KMIVMASHMQ EQEVGDGTN FVLVFAGALL ELAEELLRIG LSVSEVIEGY EIACRKAHEI LPNLVCCSAK NLRDIDEVSS LLRTSIMSKQ Y GNEVFLAK ...文字列: MLKEGAKHFS GLEEAVYRNI QACKELAQTT RTAYGPNGMN KMVINHLEKL FVTNDAATIL RELEVQHPAA KMIVMASHMQ EQEVGDGTN FVLVFAGALL ELAEELLRIG LSVSEVIEGY EIACRKAHEI LPNLVCCSAK NLRDIDEVSS LLRTSIMSKQ Y GNEVFLAK LIAQACVSIF PDSGHFNVDN IRVCKILGSG ISSSSVLHGM VFKKETEGDV TSVKDAKIAV YSCPFDGMIT ET KGTVLIK TAEELMNFSK GEENLMDAQV KAIADTGANV VVTGGKVADM ALHYANKYNI MLVRLNSKWD LRRLCKTVGA TAL PRLTPP VLEEMGHCDS VYLSEVGDTQ VVVFKHEKED GAISTIVLRG STDNLMDDIE RAVDDGVNTF KVLTRDKRLV PGGG ATEIE LAKQITSYGE TCPGLEQYAI KKFAEAFEAI PRALAENSGV KANEVISKLY AVHQEGNKNV GLDIEAEVPA VKDML EAGI LDTYLGKYWA IKLATNAAVT VLRVDQIIMA UniProtKB: T-complex protein 1 subunit theta |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.5 |
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グリッド | 詳細: unspecified |
凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | JEOL 3200FSC |
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撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 平均電子線量: 48.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD |