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- EMDB-0432: Structure of a Protein Complex -

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Basic information

Entry
Database: EMDB / ID: EMD-0432
TitleStructure of a Protein Complex
Map data
SampleNative A2AxA3A2 complex bound with MPQX
Biological speciesRattus norvegicus (Norway rat)
Methodsingle particle reconstruction / cryo EM / Resolution: 21 Å
AuthorsZhao Y / Gouaux E
CitationJournal: To Be Published
Title: Structure of a Protein Complex
Authors: Zhao Y / Chen SS / Gouaux E
History
DepositionDec 10, 2018-
Header (metadata) releaseMar 27, 2019-
UpdateMar 27, 2019-
Current statusMar 27, 2019Processing site: RCSB / Status: Hold

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Structure visualization

Downloads & links

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Map

FileOn hold for 1 year
Voxel sizeX=Y=Z: 1.72 Å
Density
Contour LevelBy AUTHOR: 0.012
Minimum - Maximum-0.010980289 - 0.041680027
Average (Standard dev.)0.000092649934 (±0.0020972567)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions320320320
Spacing320320320
CellA=B=C: 550.4 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire Native A2AxA3A2 complex bound with MPQX

EntireName: Native A2AxA3A2 complex bound with MPQX / Number of components: 1

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Component #1: protein, Native A2AxA3A2 complex bound with MPQX

ProteinName: Native A2AxA3A2 complex bound with MPQX / Recombinant expression: No
SourceSpecies: Rattus norvegicus (Norway rat)

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Experimental details

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Sample preparation

SpecimenSpecimen state: Particle / Method: cryo EM
Sample solutionSpecimen conc.: 4 mg/mL / pH: 8
Support filmunspecified
VitrificationCryogen name: ETHANE / Temperature: 295 K / Humidity: 100 %

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
ImagingMicroscope: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 54 e/Å2 / Illumination mode: FLOOD BEAM
LensImaging mode: BRIGHT FIELD
Specimen HolderModel: OTHER
CameraDetector: GATAN K2 SUMMIT (4k x 4k)

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Image processing

ProcessingMethod: single particle reconstruction / Number of projections: 10800
3D reconstructionSoftware: RELION / Resolution: 21 Å / Resolution method: FSC 0.143 CUT-OFF

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Atomic model buiding

Modeling #1Refinement protocol: rigid body / Refinement space: REAL

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