EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Nucleoplasmic checkpoint of the 40S ribosomal decoding center maturation.
ジャーナル・号・ページ	Cell Rep, Vol. 45, Issue 6, Page 117545, Year 2026
掲載日	2026年6月11日
著者	Benjamin Lau / Yi Li / Jingyi Zhu / Xianwen Ye / Paulina Fischer / Xiaying Hong / Rui Yuan / Roland Beckmann / Ed Hurt / Jingdong Cheng /
PubMed 要旨	The decoding center (DC) is a key ribosomal structure for accurate translation, assembled in a multi-step process that starts on nucleolar pre-ribosomes and ends in the cytoplasm. While late ...The decoding center (DC) is a key ribosomal structure for accurate translation, assembled in a multi-step process that starts on nucleolar pre-ribosomes and ends in the cytoplasm. While late cytoplasmic steps and their checkpoint mechanisms are well characterized, the regulation of early nucleoplasmic DC assembly is unclear. Here, we show that the essential assembly factor Rrp12 plays a central coordinating role. Using Chaetomium thermophilum and cryo-electron microscopy analyses of fifteen pre-40S intermediates, we demonstrate that Rrp12 C terminus truncation: (1) inhibits release of the Utp14-Dhr1 pair, (2) displaces Tsr1, (3) promotes premature stabilization of h28, and (4) prevents h44 formation. These defects impair final 18S rRNA processing and prematurely activate the quality control kinase Rio1. Our results reveal a nucleoplasmic checkpoint during DC formation and establish Rrp12 as a critical regulator ensuring accurate assembly and orderly ribosome maturation.
リンク	Cell Rep / PubMed:42275223 / PubMed Central
手法	EM (単粒子)
解像度	2.8 - 4.9 Å
構造データ	66677 9xa7 EMDB-66677, PDB-9xa7: Cryo-EM structure of the 90S pre-ribosome (Enp1-Rrp12 WT) from Chaetomium thermophilum, state A 手法: EM (単粒子) / 解像度: 3.2 Å 66678 9xa8 EMDB-66678, PDB-9xa8: Cryo-EM structure of the 90S pre-ribosome (Enp1-Rrp12 WT) from Chaetomium thermophilum, state B1 手法: EM (単粒子) / 解像度: 2.9 Å 66679 9xa9 EMDB-66679, PDB-9xa9: Cryo-EM structure of the pre-40S ribosome (Enp1-Rrp12 WT) from Chaetomium thermophilum, state Tsr1-1 手法: EM (単粒子) / 解像度: 3.3 Å 66680 9xaa EMDB-66680, PDB-9xaa: Cryo-EM structure of the pre-40S ribosome (Enp1-Rrp12 WT) from Chaetomium thermophilum, state Tsr1-2 手法: EM (単粒子) / 解像度: 3.1 Å 66681 9xab EMDB-66681, PDB-9xab: Cryo-EM structure of the pre-40S ribosome (Enp1-Rrp12 WT) from Chaetomium thermophilum, state Tsr1-3 手法: EM (単粒子) / 解像度: 3.2 Å 66682 9xac EMDB-66682, PDB-9xac: Cryo-EM structure of the pre-40S ribosome (Enp1-Rrp12 WT) from Chaetomium thermophilum, state Rrp12-A1 手法: EM (単粒子) / 解像度: 3.3 Å 66683 9xad EMDB-66683, PDB-9xad: Cryo-EM structure of the pre-40S ribosome (Enp1-Rrp12 WT) from Chaetomium thermophilum, state Rrp12-A2 手法: EM (単粒子) / 解像度: 3.0 Å 66684 9xae EMDB-66684, PDB-9xae: Cryo-EM structure of the pre-40S ribosome (Enp1-Rrp12 WT) from Chaetomium thermophilum, state Rio2-C 手法: EM (単粒子) / 解像度: 3.0 Å 66685 9xaf EMDB-66685, PDB-9xaf: Cryo-EM structure of the 90S pre-ribosome (Enp1-Rrp12 Mut) from Chaetomium thermophilum, state a 手法: EM (単粒子) / 解像度: 3.0 Å 66686 9xag EMDB-66686, PDB-9xag: Cryo-EM structure of the 90S pre-ribosome (Enp1-Rrp12 Mut) from Chaetomium thermophilum, state B1 手法: EM (単粒子) / 解像度: 2.8 Å 66687 9xah EMDB-66687, PDB-9xah: Cryo-EM structure of the 90S pre-ribosome (Enp1-Rrp12 Mut) from Chaetomium thermophilum, state B1* 手法: EM (単粒子) / 解像度: 3.1 Å 66688 9xai EMDB-66688, PDB-9xai: Cryo-EM structure of the pre-40S ribosome (Enp1-Rrp12 Mut) from Chaetomium thermophilum, state Tsr1-1* 手法: EM (単粒子) / 解像度: 4.1 Å 66689 9xaj EMDB-66689, PDB-9xaj: Cryo-EM structure of the pre-40S ribosome (Enp1-Rrp12 Mut) from Chaetomium thermophilum, state Rrp12-A1* 手法: EM (単粒子) / 解像度: 3.1 Å 66690 9xak EMDB-66690, PDB-9xak: Cryo-EM structure of the pre-40S ribosome (Enp1-Rrp12 Mut) from Chaetomium thermophilum, state Rrp12-A1 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-66691*: Cryo-EM structure of the pre-40S ribosome (Enp1-Rrp12 Mut ) from Chaetomium thermophilum, state Rio2-C 手法: EM (単粒子) / 解像度: 4.9 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, エネルギー貯蔵分子YM ChemComp-HOH: WATER
由来	thermochaetoides thermophila (菌類)
キーワード	RIBOSOME / 90S pre-ribosome / Rrp12 / Enp1 / ribosome biogenesis / 90S / pre-40S ribosome