EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structures of NHEJ assemblies with nucleosomes.
ジャーナル・号・ページ	Nat Commun, Year 2025
掲載日	2025年12月24日
著者	Chloe Hall / Philippe Frit / Antonia Kefala-Stavridi / Amandine Pelletier / Steven W Hardwick / Himani Amin / Matthew K Bilyard / Taiana Maia De Oliviera / Ammarah Tariq / Sayma Zahid / Dimitri Y Chirgadze / Shankar Balasubramanian / Katheryn Meek / Virginie Ropars / Jean-Baptiste Charbonnier / Mauro Modesti / Patrick Calsou / Sébastien Britton / Tom L Blundell / Thomas Schalch / Amanda K Chaplin /
PubMed 要旨	DNA double-strand breaks (DSBs) are highly deleterious lesions that can trigger cell death or carcinogenesis if unrepaired or misrepaired. In mammals, most DSBs are repaired by non-homologous end ...DNA double-strand breaks (DSBs) are highly deleterious lesions that can trigger cell death or carcinogenesis if unrepaired or misrepaired. In mammals, most DSBs are repaired by non-homologous end joining (NHEJ), which begins when Ku70/80 binds DNA ends and recruits DNA-PKcs to form the DNA-PK holoenzyme. Although recent cryo-EM studies have resolved several NHEJ assemblies, how these factors access DSBs within nucleosomes remains unclear. Here, we present cryo-EM structures of human Ku70/80 and DNA-PK bound to nucleosomes. Ku70/80 binds the DNA end and bends it away from the nucleosome core, while the Ku70 C-terminal SAP domain makes an additional, specific DNA contact. Our DNA-PK-nucleosome structure further reveals the opening of the Ku80 vWA domain, and we show that non-hydrolysable ATP promotes synapsis by stabilising the Ku80-mediated DNA-PK dimer. These structures reveal a model for DSB recognition on nucleosomal DNA and provide insights relevant to targeting NHEJ in cancer therapy.
リンク	Nat Commun / PubMed:41444611
手法	EM (単粒子)
解像度	2.94 - 5.0 Å
構造データ	52860 9igw EMDB-52860, PDB-9igw: Ku70/80 bound to 147 bp nucleosome 手法: EM (単粒子) / 解像度: 3.32 Å 52861 9igx EMDB-52861, PDB-9igx: Ku70/80 bound to 153 bp nucleosome 手法: EM (単粒子) / 解像度: 3.56 Å 52879 9q80 EMDB-52879, PDB-9q80: Ku70/80 with Ku70 linker and SAP domain bound to a 153 bp H2AX nucleosome 手法: EM (単粒子) / 解像度: 3.39 Å 52912 9q8x EMDB-52912, PDB-9q8x: Ku70/80 bound to a 153 bp H2AX nucleosome 手法: EM (単粒子) / 解像度: 2.94 Å 52958 9q9f EMDB-52958, PDB-9q9f: DNA-PK bound to a 153 bp H2AX nucleosome model 1 手法: EM (単粒子) / 解像度: 4.54 Å 53025 9qcr EMDB-53025, PDB-9qcr: DNA-PK bound to 153 bp H2AX nucleosome model 2 手法: EM (単粒子) / 解像度: 4.37 Å 53026 9qcs EMDB-53026, PDB-9qcs: Ku80 mediated DNA-PK dimer bound to 153 bp H2AX nucleosome 手法: EM (単粒子) / 解像度: 4.38 Å 53237 9qms EMDB-53237, PDB-9qms: DNA-PK bound to 153 bp H2AX nucleosome with ATPyS 手法: EM (単粒子) / 解像度: 5.0 Å
由来	homo sapiens (ヒト)
キーワード	DNA BINDING PROTEIN / DNA-binding protein / NHEJ / Ku70/80 / nucleosome