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Structure paper

TitleAssembly and architecture of endogenous NMDA receptors in adult cerebral cortex and hippocampus.
Journal, issue, pagesCell, Vol. 188, Issue 5, Page 1198-1207.e13, Year 2025
Publish dateMar 6, 2025
AuthorsMing Zhang / Juan Feng / Chun Xie / Nan Song / Chaozhi Jin / Jian Wang / Qun Zhao / Lihua Zhang / Boshuang Wang / Yidi Sun / Fei Guo / Yang Li / Shujia Zhu /
PubMed AbstractThe cerebral cortex and hippocampus are crucial brain regions for learning and memory, which depend on activity-induced synaptic plasticity involving N-methyl-ᴅ-aspartate receptors (NMDARs). ...The cerebral cortex and hippocampus are crucial brain regions for learning and memory, which depend on activity-induced synaptic plasticity involving N-methyl-ᴅ-aspartate receptors (NMDARs). However, subunit assembly and molecular architecture of endogenous NMDARs (eNMDARs) in the brain remain elusive. Using conformation- and subunit-dependent antibodies, we purified eNMDARs from adult rat cerebral cortex and hippocampus. Three major subtypes of GluN1-N2A-N2B, GluN1-N2B, and GluN1-N2A eNMDARs were resolved by cryoelectron microscopy (cryo-EM) at the resolution up to 4.2 Å. The particle ratio of these three subtypes was 9:7:4, indicating that about half of GluN2A and GluN2B subunits are incorporated into the tri-heterotetramers. Structural analysis revealed the asymmetric architecture of the GluN1-N2A-N2B receptor throughout the extracellular to the transmembrane layers. Moreover, the conformational variations between GluN1-N2B and GluN1-N2A-N2B receptors revealed the distinct biophysical properties across different eNMDAR subtypes. Our findings imply the structural and functional complexity of eNMDARs and shed light on structure-based therapeutic design targeting these eNMDARs in vivo.
External linksCell / PubMed:39855198
MethodsEM (single particle)
Resolution3.1 - 9.8 Å
Structure data

38450

8xlj

EMDB-38450, PDB-8xlj:
Structure of the native glutamine synthetase in the adult cortex and hippocampus
Method: EM (single particle) / Resolution: 3.9 Å

38451

8xlk

EMDB-38451, PDB-8xlk:
Structure of native tri-heteromeric GluN1-GluN2A-GluN2B NMDA receptor in rat cortex and hippocampus
Method: EM (single particle) / Resolution: 4.2 Å

38452

8xll

EMDB-38452, PDB-8xll:
Structure of the native 2-oxoglutarate dehydrogenase complex (OGDHC) in the adult cortex and hippocampus
Method: EM (single particle) / Resolution: 3.1 Å

61622

9jnn

EMDB-61622, PDB-9jnn:
Structure of native di-heteromeric GluN1-GluN2B NMDA receptor in rat cortex and hippocampus
Method: EM (single particle) / Resolution: 5.4 Å

EMDB-61623: Structure of native di-heteromeric GluN1-GluN2A NMDA receptor in rat cortex and hippocampus
Method: EM (single particle) / Resolution: 9.8 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-7RC:
(2R)-4-(3-phosphonopropyl)piperazine-2-carboxylic acid

Source
  • rattus norvegicus (Norway rat)
  • mus musculoides (Temminck's mouse)
  • mus musculus (house mouse)
KeywordsCYTOSOLIC PROTEIN / Native glutamine synthetase / MEMBRANE PROTEIN/IMMUNE SYSTEM / MEMBRANE PROTEIN / native NMDA receptor / adult rat cartex & hippocampus / GluN2A / GluN2B / MEMBRANE PROTEIN-IMMUNE SYSTEM complex / TRANSFERASE / 2-oxoglutarate dehydrogenase complex (OGDHC)

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