EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Phosphoantigen-induced inside-out stabilization of butyrophilin receptor complexes drives dimerization-dependent γδ TCR activation.
ジャーナル・号・ページ	Immunity, Vol. 58, Issue 7, Page 1646-11659.e5, Year 2025
掲載日	2025年7月8日
著者	Yuwei Zhu / Wenbo Gao / Jianlin Zheng / Ye Bai / Xinyu Tian / Tengjin Huang / Zebin Lu / De Dong / Anqi Zhang / Changyou Guo / Zhiwei Huang /
PubMed 要旨	Phosphoantigens (pAgs), produced by infected or cancer cells, trigger the assembly of a membrane receptor complex comprising butyrophilin (BTN) members BTN3A1 and BTN2A1, leading to the activation of ...Phosphoantigens (pAgs), produced by infected or cancer cells, trigger the assembly of a membrane receptor complex comprising butyrophilin (BTN) members BTN3A1 and BTN2A1, leading to the activation of γδ T cells. BTN3A2 or BTN3A3 forms heteromers with BTN3A1, exhibiting higher γδ T cell receptor (TCR)-stimulating activity than BTN3A1 homomers. Cryoelectron microscopy (cryo-EM) structure reveals a pAg-induced BTN2A1-BTN3A1 heterotetramer with a 2:2 stoichiometry, stabilized by interactions between the intracellular B30.2 domains and the extracellular immunoglobulin V (IgV) domains. BTN3A2 or BTN3A3 heterodimerizes with BTN3A1, forming a pAg-induced tetrameric complex with BTN2A1. However, BTN3A1 heterodimers are more stable than BTN3A1 homodimers in this interaction. Cryo-EM reveals that BTN2A1-BTN3A1-BTN3A2 binds two γδ TCR ectodomains, with one being sandwiched between the IgV domains of BTN2A1 and BTN3A2, while the other interacts with the free BTN2A1 IgV in the complex, as evidenced by functional data. Together, our findings uncover the mechanism of ligand-induced inside-out stabilization of BTN receptor complexes for dimeric activation of γδ TCR.
リンク	Immunity / PubMed:40334665
手法	EM (単粒子)
解像度	2.9 - 6.95 Å
構造データ	39874 8za6 EMDB-39874, PDB-8za6: Cryo-EM structure of the gdTCR-CD3 complex 手法: EM (単粒子) / 解像度: 3.43 Å 39875 8za9 EMDB-39875, PDB-8za9: Cryo-EM structure of HBMBPP-BTN2A1-BTN3A1 complex 手法: EM (単粒子) / 解像度: 3.7 Å 39876 8zaa EMDB-39876, PDB-8zaa: Cryo-EM structure of intracellular HBMBPP-BTN2A1-BTN3A1 complex 手法: EM (単粒子) / 解像度: 3.46 Å 39877 8zab EMDB-39877, PDB-8zab: Cryo-EM structure of ectodomains of HBMBPP-BTN2A1-BTN3A1 complex 手法: EM (単粒子) / 解像度: 3.67 Å 39950 8zd4 EMDB-39950, PDB-8zd4: Cryo-EM structure of the gdTCR-ECD 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-60027: Cryo-EM structure of the Vg9Vd2 TCR-CD3 手法: EM (単粒子) / 解像度: 6.95 Å 60577 8zyr EMDB-60577, PDB-8zyr: Cryo-EM structure of BTN2A1-BTN3A1-BTN3A3 手法: EM (単粒子) / 解像度: 4.07 Å 60591 9ii6 EMDB-60591, PDB-9ii6: Cryo-EM structure of the intracellular domains of BTN2A1-BTN3A1-BTN3A3 手法: EM (単粒子) / 解像度: 3.27 Å 60596 9iik EMDB-60596, PDB-9iik: Cryo-EM structure of BTN2A1-BTN3A1-BTN3A2 手法: EM (単粒子) / 解像度: 4.12 Å 61143 9j5j EMDB-61143, PDB-9j5j: Cryo-EM structure of BTN2A1-BTN3A1-BTN3A2 in complex with gdTCR 手法: EM (単粒子) / 解像度: 4.05 Å 61146 9j5m EMDB-61146, PDB-9j5m: Cryo-EM structure of the ectodomain of BTN2A1-BTN3A1-BTN3A2 in complex with gdTCR 手法: EM (単粒子) / 解像度: 3.94 Å
化合物	ChemComp-CLR: CHOLESTEROL / コレステロ-ル ChemComp-H6P: (2E)-4-hydroxy-3-methylbut-2-en-1-yl trihydrogen diphosphate / HMBPP ChemComp-EIP: 4-HYDROXY-3-METHYL BUTYL DIPHOSPHATE
由来	homo sapiens (ヒト)
キーワード	IMMUNE SYSTEM / complex / immune / protein