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TitleMoo19 and B2: Structures of podophages with = 9 geometry and tailspikes with esterase activity.
Journal, issue, pagesSci Adv, Vol. 10, Issue 51, Page eadt0022, Year 2024
Publish dateDec 20, 2024
AuthorsSundharraman Subramanian / Silje M Bergland Drarvik / Kendal R Tinney / Sarah M Doore / Kristin N Parent /
PubMed AbstractPodophages are, by far, the least well studied of all the bacteriophages. Despite being classified together due to their short, noncontractile tails, there is a huge amount of diversity among members ...Podophages are, by far, the least well studied of all the bacteriophages. Despite being classified together due to their short, noncontractile tails, there is a huge amount of diversity among members of this group. Of the podophages, the N4-like family is the least well studied structurally and is quite divergent from well-characterized podophages such as T7 and P22. In this work, we isolate and fully characterize two members of the family by cryo-electron microscopy, genetics, and biochemistry. We describe the capsid features of Moo19 and B2, including a decoration protein. In addition, we have fully modeled the tail machinery for both phages and identify proteins with esterase activity. Genetic knockouts of the host reveal factors specific for host attachment including key modifications to the O-antigen on the lipopolysaccharide. Moo19 and B2 are both members, yet some distinct differences in the genome and structure place them into distinct clades.
External linksSci Adv / PubMed:39693418 / PubMed Central
MethodsEM (single particle)
Resolution2.4 - 4.3 Å
Structure data

46622

9d7z

EMDB-46622, PDB-9d7z:
Shigella flexneri bacteriophage Moo19 Icosahedral Reconstruction
Method: EM (single particle) / Resolution: 3.6 Å

46623

9d80

EMDB-46623, PDB-9d80:
Shigella flexneri bacteriophage Moo19 Tail
Method: EM (single particle) / Resolution: 3.7 Å

46624

9d81

EMDB-46624, PDB-9d81:
Shigella flexneri bacteriophage Moo19 Gp82
Method: EM (single particle) / Resolution: 2.4 Å

EMDB-46625: Shigella flexneri bacteriophage Moo19 Asymmetric reconstruction
Method: EM (single particle) / Resolution: 4.3 Å

46626

9d82

EMDB-46626, PDB-9d82:
Shigella flexneri bacteriophage B2 Icosahedral Reconstruction
Method: EM (single particle) / Resolution: 3.3 Å

46627

9d83

EMDB-46627, PDB-9d83:
Shigella flexneri bacteriophage B2 tail
Method: EM (single particle) / Resolution: 3.5 Å

46628

9d84

EMDB-46628, PDB-9d84:
Shigella flexneri bacteriophage B2 Gp48 and Gp49
Method: EM (single particle) / Resolution: 2.4 Å

EMDB-46629: Shigella flexneri bacteriophage B2 asymmetric reconstruction
Method: EM (single particle) / Resolution: 4.1 Å

Source
  • shigella virus moo19
  • shigella phage b2 (virus)
KeywordsVIRUS / Moo19 / VIRAL PROTEIN / Gp82 / B2 / Gp48 & Gp49

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