EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Insights into phosphoethanolamine cellulose synthesis and secretion across the Gram-negative cell envelope.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 7798, Year 2024
掲載日	2024年9月6日
著者	Preeti Verma / Ruoya Ho / Schuyler A Chambers / Lynette Cegelski / Jochen Zimmer /
PubMed 要旨	Phosphoethanolamine (pEtN) cellulose is a naturally occurring modified cellulose produced by several Enterobacteriaceae. The minimal components of the E. coli cellulose synthase complex include the ...Phosphoethanolamine (pEtN) cellulose is a naturally occurring modified cellulose produced by several Enterobacteriaceae. The minimal components of the E. coli cellulose synthase complex include the catalytically active BcsA enzyme, a hexameric semicircle of the periplasmic BcsB protein, and the outer membrane (OM)-integrated BcsC subunit containing periplasmic tetratricopeptide repeats (TPR). Additional subunits include BcsG, a membrane-anchored periplasmic pEtN transferase associated with BcsA, and BcsZ, a periplasmic cellulase of unknown biological function. While cellulose synthesis and translocation by BcsA are well described, little is known about its pEtN modification and translocation across the cell envelope. We show that the N-terminal cytosolic domain of BcsA positions three BcsG copies near the nascent cellulose polymer. Further, the semicircle's terminal BcsB subunit tethers the N-terminus of a single BcsC protein in a trans-envelope secretion system. BcsC's TPR motifs bind a putative cello-oligosaccharide near the entrance to its OM pore. Additionally, we show that only the hydrolytic activity of BcsZ but not the subunit itself is necessary for cellulose secretion, suggesting a secretion mechanism based on enzymatic removal of translocation incompetent cellulose. Lastly, protein engineering introduces cellulose pEtN modification in orthogonal cellulose biosynthetic systems. These findings advance our understanding of pEtN cellulose modification and secretion.
リンク	Nat Commun / PubMed:39242554 / PubMed Central
手法	EM (単粒子)
解像度	2.65 - 5.71 Å
構造データ	44334 9b87 EMDB-44334, PDB-9b87: Tetrameric cryo-EM structure of E. coli BcsZ 手法: EM (単粒子) / 解像度: 2.65 Å 44336 9b8a EMDB-44336, PDB-9b8a: Cryo-EM structure of E. coli cellulose synthase subunit C 手法: EM (単粒子) / 解像度: 3.4 Å 44345 9b8h EMDB-44345, PDB-9b8h: Cryo-EM structure of E. coli cellulose synthase subunit C with cellotetraose 手法: EM (単粒子) / 解像度: 3.8 Å 44346 9b8i EMDB-44346, PDB-9b8i: Cryo-EM structure of the E. coli cellulose synthase BcsB-BcsC fusion protein 手法: EM (単粒子) / 解像度: 3.18 Å 44359 9b8v EMDB-44359: Cryo-EM map of the E. coli cellulose synthase BcsAG3B6 complex PDB-9b8v: AlphaFold2 informed cryo-EM model of the E. coli cellulose synthase BcsAG3B6 complex 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-44793: E. coli cellulose synthase BcsAG3B6 complex - Consensus map 手法: EM (単粒子) / 解像度: 5.67 Å EMDB-44794: E. coli cellulose synthase BcsAG3B6 complex - BcsB focused map 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-44795: E.coli cellulose synthase BcsAG3B6 complex - BcsAG focused map 手法: EM (単粒子) / 解像度: 5.71 Å
由来	escherichia coli (大腸菌)
キーワード	HYDROLASE / Cellulase / Endoglucanase / GH-8 family / Carbohydrate-active enzyme / MEMBRANE PROTEIN / Porin / bacterial outer membrane protein / Tetra-tricopeptide (TPR) repeats / Cellulose exporter BcsC / BcsC outer membrane protein / cellooligosaccharide / STRUCTURAL PROTEIN / bacterial cellulose synthesis / cellulose export / outer membrane porin / periplasmic protein / TRANSFERASE / phosphoethanolamine (pEtN) / catalytic BcsA-B / c-di-GMP binding protein / membrane proteins