EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of closed groove scrambling by a TMEM16 protein.
ジャーナル・号・ページ	Nat Struct Mol Biol, Year 2024
掲載日	2024年4月29日
著者	Zhang Feng / Omar E Alvarenga / Alessio Accardi /
PubMed 要旨	Activation of Ca-dependent TMEM16 scramblases induces phosphatidylserine externalization, a key step in multiple signaling processes. Current models suggest that the TMEM16s scramble lipids by ...Activation of Ca-dependent TMEM16 scramblases induces phosphatidylserine externalization, a key step in multiple signaling processes. Current models suggest that the TMEM16s scramble lipids by deforming the membrane near a hydrophilic groove and that Ca dependence arises from the different association of lipids with an open or closed groove. However, the molecular rearrangements underlying groove opening and how lipids reorganize outside the closed groove remain unknown. Here we directly visualize how lipids associate at the closed groove of Ca-bound fungal nhTMEM16 in nanodiscs using cryo-EM. Functional experiments pinpoint lipid-protein interaction sites critical for closed groove scrambling. Structural and functional analyses suggest groove opening entails the sequential appearance of two π-helical turns in the groove-lining TM6 helix and identify critical rearrangements. Finally, we show that the choice of scaffold protein and lipids affects the conformations of nhTMEM16 and their distribution, highlighting a key role of these factors in cryo-EM structure determination.
リンク	Nat Struct Mol Biol / PubMed:38684930
手法	EM (単粒子)
解像度	2.64 - 4.07 Å
構造データ	41453 8toi EMDB-41453, PDB-8toi: nhTMEM16 lipid scramblase in lipid nanodiscs with MSP1E3 scaffold protein in the presence of Ca2+ (closed state) 手法: EM (単粒子) / 解像度: 2.64 Å 41454 8tok EMDB-41454, PDB-8tok: nhTMEM16 lipid scramblase in lipid nanodiscs with MSP2N2 scaffold protein in the presence of Ca2+ (open state) 手法: EM (単粒子) / 解像度: 3.84 Å 41455 8tol EMDB-41455, PDB-8tol: nhTMEM16 lipid scramblase in lipid nanodiscs with MSP1E3 scaffold protein in the presence of Ca2+ (open state) 手法: EM (単粒子) / 解像度: 2.85 Å EMDB-41457: nhTMEM16 lipid scramblase in lipid nanodiscs with MSP1E3 scaffold protein in the presence of Ca2+ (closed state) (consensus map) 手法: EM (単粒子) / 解像度: 2.64 Å EMDB-41458: nhTMEM16 lipid scramblase in lipid nanodiscs with MSP1E3 scaffold protein in the presence of Ca2+ (closed state)(local refined monomer map) 手法: EM (単粒子) / 解像度: 2.64 Å 41477 8tpm EMDB-41477, PDB-8tpm: nhTMEM16 lipid scramblase in lipid nanodiscs with MSP1E3 scaffold protein in the absence of Ca2+ 手法: EM (単粒子) / 解像度: 2.93 Å 41478 8tpn EMDB-41478, PDB-8tpn: nhTMEM16 lipid scramblase in lipid nanodiscs with MSP2N2 scaffold protein in the presence of Ca2+ (intermediate state) 手法: EM (単粒子) / 解像度: 4.07 Å 41479 8tpo EMDB-41479, PDB-8tpo: nhTMEM16 R432A mutant in lipid nanodiscs with MSP1E3 scaffold protein in the presence of Ca2+ 手法: EM (単粒子) / 解像度: 3.59 Å 41480 8tpp EMDB-41480, PDB-8tpp: nhTMEM16 R432A mutant in lipid nanodiscs with MSP2N2 scaffold protein in the presence of Ca2+ 手法: EM (単粒子) / 解像度: 3.64 Å 41481 8tpq EMDB-41481, PDB-8tpq: nhTMEM16 A444P mutant in lipid nanodiscs with MSP1E3 scaffold protein in the presence of Ca2+ (long TM6) 手法: EM (単粒子) / 解像度: 3.35 Å 41482 8tpr EMDB-41482, PDB-8tpr: nhTMEM16 A444P mutant in lipid nanodiscs with MSP1E3 scaffold protein in the presence of Ca2+ (short TM6) 手法: EM (単粒子) / 解像度: 2.85 Å 41483 8tps EMDB-41483, PDB-8tps: nhTMEM16 A444P mutant in lipid nanodiscs with MSP1E3 scaffold protein in the presence of Ca2+ (bent TM6) 手法: EM (単粒子) / 解像度: 2.97 Å 41484 8tpt EMDB-41484, PDB-8tpt: nhTMEM16 A444P mutant in lipid nanodiscs with MSP1E3 scaffold protein in the presence of Ca2+ (long TM6/short TM6) 手法: EM (単粒子) / 解像度: 3.01 Å
化合物	ChemComp-CA: Unknown entry / カルシウムジカチオン ChemComp-PGW: (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl / POPG / リン脂質*YM / Phosphatidylglycerol
由来	fusarium vanettenii 77-13-4 (菌類) fusarium vanettenii (菌類)
キーワード	LIPID TRANSPORT / membrane protein (膜タンパク質) / lipid scramblase / TMEM16