Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Cryo-EM architecture of a near-native stretch-sensitive membrane microdomain.
Journal, issue, pages	Nature, Vol. 632, Issue 8025, Page 664-671, Year 2024
Publish date	Jul 24, 2024
Authors	Jennifer M Kefauver / Markku Hakala / Luoming Zou / Josephine Alba / Javier Espadas / Maria G Tettamanti / Jelena Gajić / Caroline Gabus / Pablo Campomanes / Leandro F Estrozi / Nesli E Sen / Stefano Vanni / Aurélien Roux / Ambroise Desfosses / Robbie Loewith /
PubMed Abstract	Biological membranes are partitioned into functional zones termed membrane microdomains, which contain specific lipids and proteins. The composition and organization of membrane microdomains remain ...Biological membranes are partitioned into functional zones termed membrane microdomains, which contain specific lipids and proteins. The composition and organization of membrane microdomains remain controversial because few techniques are available that allow the visualization of lipids in situ without disrupting their native behaviour. The yeast eisosome, composed of the BAR-domain proteins Pil1 and Lsp1 (hereafter, Pil1/Lsp1), scaffolds a membrane compartment that senses and responds to mechanical stress by flattening and releasing sequestered factors. Here we isolated near-native eisosomes as helical tubules made up of a lattice of Pil1/Lsp1 bound to plasma membrane lipids, and solved their structures by helical reconstruction. Our structures reveal a striking organization of membrane lipids, and, using in vitro reconstitutions and molecular dynamics simulations, we confirmed the positioning of individual PI(4,5)P, phosphatidylserine and sterol molecules sequestered beneath the Pil1/Lsp1 coat. Three-dimensional variability analysis of the native-source eisosomes revealed a dynamic stretching of the Pil1/Lsp1 lattice that affects the sequestration of these lipids. Collectively, our results support a mechanism in which stretching of the Pil1/Lsp1 lattice liberates lipids that would otherwise be anchored by the Pil1/Lsp1 coat, and thus provide mechanistic insight into how eisosome BAR-domain proteins create a mechanosensitive membrane microdomain.
External links	Nature / PubMed:39048819 / PubMed Central
Methods	EM (single particle) / EM (helical sym.)
Resolution	3.2 - 3.86 Å
Structure data	18307 8qb7 8qb8 EMDB-18307: Native eisosome lattice bound to plasma membrane microdomain PDB-8qb7: Pil1 in native eisosome lattice bound to plasma membrane microdomain PDB-8qb8: Lsp1 in native eisosome lattice bound to plasma membrane microdomain Method: EM (single particle) / Resolution: 3.2 Å 18308 8qb9 EMDB-18308, PDB-8qb9: Helical reconstruction of yeast eisosome protein Pil1 bound to membrane composed of lipid mixture -PIP2/+sterol (DOPC, DOPE, DOPS, cholesterol 30:20:20:30) Method: EM (helical sym.) / Resolution: 3.35 Å 18309 8qbb EMDB-18309, PDB-8qbb: Helical reconstruction of yeast eisosome protein Pil1 bound to membrane composed of lipid mixture +PIP2/-sterol (DOPC, DOPE, DOPS, PI(4,5)P2 50:20:20:10) Method: EM (helical sym.) / Resolution: 3.86 Å 18310 8qbd EMDB-18310, PDB-8qbd: Helical reconstruction of yeast eisosome protein Pil1 bound to membrane composed of lipid mixture +PIP2/+sterol (DOPC, DOPE, DOPS, cholesterol, PI(4,5)P2 35:20:20:15:10) Method: EM (helical sym.) / Resolution: 3.61 Å 18311 8qbe 8qbf EMDB-18311: Compact state - Native eisosome lattice bound to plasma membrane microdomain PDB-8qbe: Compact state - Pil1 in native eisosome lattice bound to plasma membrane microdomain PDB-8qbf: Compact state - Pil1 dimer with lipid headgroups fitted in native eisosome lattice bound to plasma membrane microdomain Method: EM (single particle) / Resolution: 3.67 Å 18312 8qbg EMDB-18312: Stretched state - Native eisosome lattice bound to plasma membrane microdomain PDB-8qbg: Stretched state - Pil1 in native eisosome lattice bound to plasma membrane microdomain Method: EM (single particle) / Resolution: 3.64 Å EMDB-19822: Helical reconstruction of yeast eisosome protein Pil1 bound to membrane composed of lipid mixture +PIP2/+bromosterol (DOPC, DOPE, DOPS, bromo-ergosterol, PI(4,5)P2 35:20:20:15:10) Method: EM (helical sym.) / Resolution: 3.85 Å
Chemicals	ChemComp-I3P: D-MYO-INOSITOL-1,4,5-TRIPHOSPHATE ChemComp-P5S: O-[(R)-{[(2R)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-L-serine ChemComp-SEP: PHOSPHOSERINE
Source	saccharomyces cerevisiae (brewer's yeast)
Keywords	LIPID BINDING PROTEIN / BAR domain / plasma membrane microdomain / membrane curvature / native biochemistry / lipid reconstitution / membrane microdomain