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TitleStructural basis for translational control by the human 48S initiation complex from codon scanning toward subunit joining
Journal, issue, pagesNat. Struct. Mol. Biol., Year 2024
Publish dateJun 22, 2023 (structure data deposition date)
AuthorsPetrychenko V / Yi S-H / Liedtke D / Peng BZ / Rodnina MV / Fischer N
External linksNat. Struct. Mol. Biol. / Search PubMed
MethodsEM (single particle)
Resolution2.9 - 3.7 Å
Structure data

17696
EMDB entry, No image

EMDB-17696, PDB-8pj1:
Structure of human 48S translation initiation complex in open codon scanning state (48S-1)
Method: EM (single particle) / Resolution: 3.4 Å

17697
EMDB entry, No image

EMDB-17697, PDB-8pj2:
Structure of human 48S translation initiation complex in AUG recognition state after eIF5-induced GTP hydrolysis by eIF2 (48S-2)
Method: EM (single particle) / Resolution: 3.4 Å

17698
EMDB entry, No image

EMDB-17698, PDB-8pj3:
Structure of human 48S translation initiation complex upon transfer of initiator tRNA to eIF5B (48S-3)
Method: EM (single particle) / Resolution: 3.7 Å

17699
EMDB entry, No image

EMDB-17699, PDB-8pj4:
Structure of human 48S translation initiation complex after eIF5 release (48S-4)
Method: EM (single particle) / Resolution: 3.2 Å

17700
EMDB entry, No image

EMDB-17700, PDB-8pj5:
Structure of human 48S translation initiation complex after eIF2 release prior 60S subunit joining (48S-5)
Method: EM (single particle) / Resolution: 2.9 Å

17701
EMDB entry, No image

EMDB-17701, PDB-8pj6:
Structure of human 48S translation initiation complex with initiator tRNA, eIF1A and eIF3 (off-pathway)
Method: EM (single particle) / Resolution: 2.9 Å

19128
EMDB entry, No image

EMDB-19128, PDB-8rg0:
Structure of human eIF3 core from closed 48S translation initiation complex
Method: EM (single particle) / Resolution: 3.4 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-MET:
METHIONINE

ChemComp-NA:
Unknown entry

Source
  • homo sapiens (human)
KeywordsRIBOSOME / TRANSLATION / initiation / 48S / eIF / human / eukaryotic / factor / codon / scanning / open / reading / closed

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