Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structural basis for assembly and lipid-mediated gating of LRRC8A:C volume-regulated anion channels.
Journal, issue, pages	Nat Struct Mol Biol, Vol. 30, Issue 6, Page 841-852, Year 2023
Publish date	Mar 16, 2023
Authors	David M Kern / Julia Bleier / Somnath Mukherjee / Jennifer M Hill / Anthony A Kossiakoff / Ehud Y Isacoff / Stephen G Brohawn /
PubMed Abstract	Leucine-rich repeat-containing protein 8 (LRRC8) family members form volume-regulated anion channels activated by hypoosmotic cell swelling. LRRC8 channels are ubiquitously expressed in vertebrate ...Leucine-rich repeat-containing protein 8 (LRRC8) family members form volume-regulated anion channels activated by hypoosmotic cell swelling. LRRC8 channels are ubiquitously expressed in vertebrate cells as heteromeric assemblies of LRRC8A (SWELL1) and LRRC8B-E subunits. Channels of different subunit composition have distinct properties that explain the functional diversity of LRRC8 currents across cell types. However, the basis for heteromeric LRRC8 channel assembly and function is unknown. Here we leverage a fiducial-tagging strategy to determine single-particle cryo-EM structures of heterohexameric LRRC8A:C channels in multiple conformations. Compared to homomers, LRRC8A:C channels show pronounced differences in architecture due to heterotypic LRR interactions that displace subunits away from the conduction axis and poise the channel for activation. Structures and functional studies further reveal that lipids embedded in the channel pore block ion conduction in the closed state. These results provide insight into determinants for heteromeric LRRC8 channel assembly, activity and gating by lipids.
External links	Nat Struct Mol Biol / PubMed:36928458
Methods	EM (single particle)
Resolution	2.95 - 4.32 Å
Structure data	27674 8dr8 EMDB-27674, PDB-8dr8: LRRC8A:C conformation 2 (oblong) top mask Method: EM (single particle) / Resolution: 3.04 Å 27675 8dra EMDB-27675, PDB-8dra: LRRC8A:C conformation 2 (oblong) LRR mask Method: EM (single particle) / Resolution: 3.98 Å 27676 8dre EMDB-27676, PDB-8dre: LRRC8A:C conformation 2 (oblong) Method: EM (single particle) / Resolution: 3.18 Å 27677 8drk EMDB-27677, PDB-8drk: LRRC8A:C conformation 1 (round) top focus Method: EM (single particle) / Resolution: 2.95 Å 27678 8drn EMDB-27678, PDB-8drn: LRRC8A:C conformation 1 (round) LRR focus 1 Method: EM (single particle) / Resolution: 4.12 Å 27679 8dro EMDB-27679, PDB-8dro: LRRC8A:C conformation 1 (round) LRR focus 2 Method: EM (single particle) / Resolution: 4.06 Å 27681 8drq EMDB-27681, PDB-8drq: LRRC8A:C conformation 1 (round) LRR focus 3 Method: EM (single particle) / Resolution: 4.16 Å 27682 8ds3 EMDB-27682, PDB-8ds3: LRRC8A:C conformation 1 (round) Method: EM (single particle) / Resolution: 3.07 Å 27686 8ds9 EMDB-27686, PDB-8ds9: LRRC8A:C in MSPE3D1 nanodisc top focus Method: EM (single particle) / Resolution: 3.17 Å 27687 8dsa EMDB-27687, PDB-8dsa: LRRC8A:C in MSP1E3D1 nanodisc Method: EM (single particle) / Resolution: 3.48 Å 28894 8f74 EMDB-28894, PDB-8f74: LRRC8A(T48D):C conformation 2 top focus Method: EM (single particle) / Resolution: 3.1 Å 28895 8f75 EMDB-28895, PDB-8f75: LRRC8A(T48D):C conformation 2 LRR focus Method: EM (single particle) / Resolution: 4.0 Å 28897 8f77 EMDB-28897, PDB-8f77: LRRC8A(T48D):C conformation 2 top focus Method: EM (single particle) / Resolution: 3.15 Å 28898 8f79 EMDB-28898, PDB-8f79: LRRC8A(T48D):C conformation 2 top focus Method: EM (single particle) / Resolution: 3.15 Å 28901 8f7b EMDB-28901, PDB-8f7b: LRRC8A(T48D):C conformation 2 top focus Method: EM (single particle) / Resolution: 3.15 Å 28903 8f7d EMDB-28903, PDB-8f7d: LRRC8A(T48D):C conformation 2 top focus Method: EM (single particle) / Resolution: 4.2 Å 28904 8f7e EMDB-28904, PDB-8f7e: LRRC8A(T48D):C conformation 2 top focus Method: EM (single particle) / Resolution: 4.13 Å 28905 8f7j EMDB-28905, PDB-8f7j: LRRC8A(T48D):C conformation 2 top focus Method: EM (single particle) / Resolution: 4.32 Å
Chemicals	ChemComp-PEE: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine / DOPE, phospholipidYM / Discrete optimized protein energy ChemComp-HOH*: WATER / Water
Source	mus musculus (house mouse)
Keywords	MEMBRANE PROTEIN / ION CHANNEL / VOLUME-REGULATION