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Structure paper

TitleStructural basis for the activation of the lipid scramblase TMEM16F.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 6692, Year 2022
Publish dateNov 5, 2022
AuthorsMelanie Arndt / Carolina Alvadia / Monique S Straub / Vanessa Clerico Mosina / Cristina Paulino / Raimund Dutzler /
PubMed AbstractTMEM16F, a member of the conserved TMEM16 family, plays a central role in the initiation of blood coagulation and the fusion of trophoblasts. The protein mediates passive ion and lipid transport in ...TMEM16F, a member of the conserved TMEM16 family, plays a central role in the initiation of blood coagulation and the fusion of trophoblasts. The protein mediates passive ion and lipid transport in response to an increase in intracellular Ca. However, the mechanism of how the protein facilitates both processes has remained elusive. Here we investigate the basis for TMEM16F activation. In a screen of residues lining the proposed site of conduction, we identify mutants with strongly activating phenotype. Structures of these mutants determined herein by cryo-electron microscopy show major rearrangements leading to the exposure of hydrophilic patches to the membrane, whose distortion facilitates lipid diffusion. The concomitant opening of a pore promotes ion conduction in the same protein conformation. Our work has revealed a mechanism that is distinct for this branch of the family and that will aid the development of a specific pharmacology for a promising drug target.
External linksNat Commun / PubMed:36335104 / PubMed Central
MethodsEM (single particle)
Resolution2.93 - 3.49 Å
Structure data

15913

8b8g

EMDB-15913, PDB-8b8g:
Cryo-EM structure of Ca2+-free mTMEM16F F518H mutant in Digitonin
Method: EM (single particle) / Resolution: 3.39 Å

15914

8b8j

EMDB-15914, PDB-8b8j:
Cryo-EM structure of Ca2+-bound mTMEM16F F518H mutant in Digitonin
Method: EM (single particle) / Resolution: 2.96 Å

15916

8b8k

EMDB-15916, PDB-8b8k:
Cryo-EM structure of Ca2+-bound mTMEM16F N562A mutant in Digitonin closed/closed
Method: EM (single particle) / Resolution: 3.01 Å

15917

8b8m

EMDB-15917, PDB-8b8m:
Cryo-EM structure of Ca2+-bound mTMEM16F N562A mutant in Digitonin open/closed
Method: EM (single particle) / Resolution: 3.49 Å

15919

8b8q

EMDB-15919, PDB-8b8q:
Structure of mTMEM16F in lipid Nanodiscs in the presence of Ca2+
Method: EM (single particle) / Resolution: 2.94 Å

15958

8bc0

EMDB-15958, PDB-8bc0:
Cryo-EM structure of Ca2+-bound mTMEM16F F518A Q623A mutant in GDN open/closed
Method: EM (single particle) / Resolution: 3.09 Å

15959

8bc1

EMDB-15959, PDB-8bc1:
Cryo-EM Structure of Ca2+-bound mTMEM16F F518A_Q623A mutant in GDN
Method: EM (single particle) / Resolution: 2.93 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-P1O:
1,2-DIDECANOYL-SN-GLYCERO-3-PHOSPHOCHOLINE / DDPC, phospholipid*YM

Source
  • mus musculus (house mouse)
KeywordsMEMBRANE PROTEIN / Lipid Transport / Lipid Scrambling / Ion Channel / Plasma Membrane / Blood Clotting / Exocytosis / Membrane Fusion / Lipid Scramblase / Blood Coagulation / Viral Entry / Cell Fusion

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