EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of dynein-dynactin on microtubules shows tandem adaptor binding.
ジャーナル・号・ページ	Nature, Vol. 610, Issue 7930, Page 212-216, Year 2022
掲載日	2022年9月7日
著者	Sami Chaaban / Andrew P Carter /
PubMed 要旨	Cytoplasmic dynein is a microtubule motor that is activated by its cofactor dynactin and a coiled-coil cargo adaptor. Up to two dynein dimers can be recruited per dynactin, and interactions between ...Cytoplasmic dynein is a microtubule motor that is activated by its cofactor dynactin and a coiled-coil cargo adaptor. Up to two dynein dimers can be recruited per dynactin, and interactions between them affect their combined motile behaviour. Different coiled-coil adaptors are linked to different cargos, and some share motifs known to contact sites on dynein and dynactin. There is limited structural information on how the resulting complex interacts with microtubules and how adaptors are recruited. Here we develop a cryo-electron microscopy processing pipeline to solve the high-resolution structure of dynein-dynactin and the adaptor BICDR1 bound to microtubules. This reveals the asymmetric interactions between neighbouring dynein motor domains and how they relate to motile behaviour. We found that two adaptors occupy the complex. Both adaptors make similar interactions with the dyneins but diverge in their contacts with each other and dynactin. Our structure has implications for the stability and stoichiometry of motor recruitment by cargos.
リンク	Nature / PubMed:36071160 / PubMed Central
手法	EM (単粒子)
解像度	3.3 - 20.0 Å
構造データ	14549 7z8f EMDB-14549, PDB-7z8f: Composite structure of dynein-dynactin-BICDR on microtubules 手法: EM (単粒子) / 解像度: 20.0 Å 14550 7z8g EMDB-14550, PDB-7z8g: Cytoplasmic dynein-1 motor domain 手法: EM (単粒子) / 解像度: 3.52 Å 14551 7z8h EMDB-14551, PDB-7z8h: Cytoplasmic dynein-1 motor domain AAA1, AAA2, and AAA3 subunits 手法: EM (単粒子) / 解像度: 3.41 Å 14552 7z8i EMDB-14552, PDB-7z8i: The barbed end complex of dynactin bound to BICDR1 and the cytoplasmic dynein tails (A2, B1, B2) 手法: EM (単粒子) / 解像度: 3.3 Å 14553 7z8j EMDB-14553, PDB-7z8j: Cytoplasmic dynein (A2) bound to BICDR1 手法: EM (単粒子) / 解像度: 3.93 Å 14555 7z8k EMDB-14555, PDB-7z8k: Cytoplasmic dynein (A1) bound to BICDR1 手法: EM (単粒子) / 解像度: 4.37 Å 14556 7z8l EMDB-14556, PDB-7z8l: Cytoplasmic dynein light intermediate chain (B1) bound to the motor domain (A2). 手法: EM (単粒子) / 解像度: 4.9 Å 14559 7z8m EMDB-14559, PDB-7z8m: The pointed end complex of dynactin bound to BICDR1 手法: EM (単粒子) / 解像度: 3.37 Å EMDB-15396: Consensus map of dynein-dynactin-BICDR on microtubules 手法: EM (単粒子) / 解像度: 6.33 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-ZN: Unknown entry ChemComp-ANP: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP / AMP-PNP, エネルギー貯蔵分子類似体*YM
由来	homo sapiens (ヒト) sus scrofa (ブタ) mus musculus (ハツカネズミ)
キーワード	STRUCTURAL PROTEIN / Dynein / dynactin / cargo transport / activating adaptor / cytoskeleton