Movie
Controller
Structure viewers
About Yorodumi Papers
+Search query
-Structure paper
| Title | A molecular switch modulates assembly and host factor binding of the HIV-1 capsid. |
|---|---|
| Journal, issue, pages | Nat Struct Mol Biol, Vol. 30, Issue 3, Page 383-390, Year 2023 |
| Publish date | Feb 9, 2023 |
 Authors | Randall T Schirra / Nayara F B Dos Santos / Kaneil K Zadrozny / Iga Kucharska / Barbie K Ganser-Pornillos / Owen Pornillos /   |
| PubMed Abstract | The HIV-1 capsid is a fullerene cone made of quasi-equivalent hexamers and pentamers of the viral CA protein. Typically, quasi-equivalent assembly of viral capsid subunits is controlled by a ...The HIV-1 capsid is a fullerene cone made of quasi-equivalent hexamers and pentamers of the viral CA protein. Typically, quasi-equivalent assembly of viral capsid subunits is controlled by a molecular switch. Here, we identify a Thr-Val-Gly-Gly motif that modulates CA hexamer/pentamer switching by folding into a 3 helix in the pentamer and random coil in the hexamer. Manipulating the coil/helix configuration of the motif allowed us to control pentamer and hexamer formation in a predictable manner, thus proving its function as a molecular switch. Importantly, the switch also remodels the common binding site for host factors that are critical for viral replication and the new ultra-potent HIV-1 inhibitor lenacapavir. This study reveals that a critical assembly element also modulates the post-assembly and viral replication functions of the HIV-1 capsid and provides new insights on capsid function and inhibition. |
 External links | Nat Struct Mol Biol / PubMed:36759579 / PubMed Central |
| Methods | EM (single particle) |
| Resolution | 2.2 - 3.9 Å |
| Structure data | EMDB-26715, PDB-7urn: EMDB-26718, PDB-7urt: EMDB-28054, PDB-8eep: EMDB-28057, PDB-8eet: EMDB-28186, PDB-8ejl: |
| Chemicals |  ChemComp-IHP: |
| Source |
|
 Keywords |  VIRUS LIKE PARTICLE / HIV-1 / capsid / declination / pentamer / hexamer / VIRAL PROTEIN |
