Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Intrinsic curvature of the HIV-1 CA hexamer underlies capsid topology and interaction with cyclophilin A.
Journal, issue, pages	Nat Struct Mol Biol, Vol. 27, Issue 9, Page 855-862, Year 2020
Publish date	Aug 3, 2020
Authors	Tao Ni / Samuel Gerard / Gongpu Zhao / Kyle Dent / Jiying Ning / Jing Zhou / Jiong Shi / Jordan Anderson-Daniels / Wen Li / Sooin Jang / Alan N Engelman / Christopher Aiken / Peijun Zhang /
PubMed Abstract	The mature retrovirus capsid consists of a variably curved lattice of capsid protein (CA) hexamers and pentamers. High-resolution structures of the curved assembly, or in complex with host factors, ...The mature retrovirus capsid consists of a variably curved lattice of capsid protein (CA) hexamers and pentamers. High-resolution structures of the curved assembly, or in complex with host factors, have not been available. By devising cryo-EM methodologies for exceedingly flexible and pleomorphic assemblies, we have determined cryo-EM structures of apo-CA hexamers and in complex with cyclophilin A (CypA) at near-atomic resolutions. The CA hexamers are intrinsically curved, flexible and asymmetric, revealing the capsomere and not the previously touted dimer or trimer interfaces as the key contributor to capsid curvature. CypA recognizes specific geometries of the curved lattice, simultaneously interacting with three CA protomers from adjacent hexamers via two noncanonical interfaces, thus stabilizing the capsid. By determining multiple structures from various helical symmetries, we further revealed the essential plasticity of the CA molecule, which allows formation of continuously curved conical capsids and the mechanism of capsid pattern sensing by CypA.
External links	Nat Struct Mol Biol / PubMed:32747784 / PubMed Central
Methods	EM (helical sym.) / EM (single particle)
Resolution	3.5 - 6.9 Å
Structure data	10226 6skk EMDB-10226, PDB-6skk: Structure of the native full-length HIV-1 capsid protein in helical assembly (-13,8) Method: EM (helical sym.) / Resolution: 3.6 Å 10228 6skm EMDB-10228, PDB-6skm: Structure of the native full-length HIV-1 capsid protein A92E in helical assembly (-13,12) Method: EM (helical sym.) / Resolution: 4.9 Å 10229 6skn EMDB-10229, PDB-6skn: Structure of the native full-length HIV-1 capsid protein in helical assembly (-13,8) Method: EM (helical sym.) / Resolution: 4.5 Å 10239 6slq EMDB-10239, PDB-6slq: Structure of the native full-length HIV-1 capsid protein A92E in helical assembly (-12,11) Method: EM (helical sym.) / Resolution: 4.4 Å 10240 6slu EMDB-10240, PDB-6slu: Structure of the native full-length HIV-1 capsid protein A92E in helical assembly (-13,11) Method: EM (helical sym.) / Resolution: 4.7 Å 10246 6smu EMDB-10246, PDB-6smu: Structure of the native full-length HIV-1 capsid protein in helical assembly (-13,12) Method: EM (helical sym.) / Resolution: 5.0 Å 10738 6y9v EMDB-10738, PDB-6y9v: Structure of the native full-length HIV-1 capsid protein in complex with Cyclophilin A from helical assembly (-8,13) Method: EM (single particle) / Resolution: 6.9 Å 10739 6y9w EMDB-10739, PDB-6y9w: Structure of the native full-length HIV-1 capsid protein in complex with Cyclophilin A from helical assembly (-13,8) Method: EM (single particle) / Resolution: 4.1 Å 10740 6y9x EMDB-10740, PDB-6y9x: Structure of the native full-length HIV-1 capsid protein in complex with Cyclophilin A from helical assembly (-13,7) Method: EM (single particle) / Resolution: 4.4 Å 10741 6y9y EMDB-10741, PDB-6y9y: Structure of the native full-length HIV-1 capsid protein in complex with Cyclophilin A from helical assembly (-7,13) Method: EM (single particle) / Resolution: 6.1 Å 10742 6y9z EMDB-10742, PDB-6y9z: Structure of the native full-length HIV-1 capsid protein in complex with Cyclophilin A from helical assembly (-13,9) Method: EM (single particle) / Resolution: 4.8 Å 10816 6yj5 EMDB-10816, PDB-6yj5: Focused refinement cryo-EM structure of the yeast mitochondrial complex I sub-stoichiometric sulfur transferase subunit Method: EM (single particle) / Resolution: 3.5 Å 11176 6zdj EMDB-11176, PDB-6zdj: Structure of the native full-length HIV-1 capsid protein in complex with Cyclophilin A from helical assembly (-13,10) Method: EM (single particle) / Resolution: 5.8 Å
Source	human immunodeficiency virus 1 homo sapiens (human) yarrowia lipolytica (yeast)
Keywords	VIRAL PROTEIN / HIV / capsid / hexamer / helical assembly / curvature / TRANSFERASE / NADH:Ubiquinone Oxidoreductase / sulfur transferase / sub-stoichiometric / complex I