EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Intrinsic curvature of the HIV-1 CA hexamer underlies capsid topology and interaction with cyclophilin A.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 27, Issue 9, Page 855-862, Year 2020
掲載日	2020年8月3日
著者	Tao Ni / Samuel Gerard / Gongpu Zhao / Kyle Dent / Jiying Ning / Jing Zhou / Jiong Shi / Jordan Anderson-Daniels / Wen Li / Sooin Jang / Alan N Engelman / Christopher Aiken / Peijun Zhang /
PubMed 要旨	The mature retrovirus capsid consists of a variably curved lattice of capsid protein (CA) hexamers and pentamers. High-resolution structures of the curved assembly, or in complex with host factors, ...The mature retrovirus capsid consists of a variably curved lattice of capsid protein (CA) hexamers and pentamers. High-resolution structures of the curved assembly, or in complex with host factors, have not been available. By devising cryo-EM methodologies for exceedingly flexible and pleomorphic assemblies, we have determined cryo-EM structures of apo-CA hexamers and in complex with cyclophilin A (CypA) at near-atomic resolutions. The CA hexamers are intrinsically curved, flexible and asymmetric, revealing the capsomere and not the previously touted dimer or trimer interfaces as the key contributor to capsid curvature. CypA recognizes specific geometries of the curved lattice, simultaneously interacting with three CA protomers from adjacent hexamers via two noncanonical interfaces, thus stabilizing the capsid. By determining multiple structures from various helical symmetries, we further revealed the essential plasticity of the CA molecule, which allows formation of continuously curved conical capsids and the mechanism of capsid pattern sensing by CypA.
リンク	Nat Struct Mol Biol / PubMed:32747784 / PubMed Central
手法	EM (らせん対称) / EM (単粒子)
解像度	3.5 - 6.9 Å
構造データ	10226 6skk EMDB-10226, PDB-6skk: Structure of the native full-length HIV-1 capsid protein in helical assembly (-13,8) 手法: EM (らせん対称) / 解像度: 3.6 Å 10228 6skm EMDB-10228, PDB-6skm: Structure of the native full-length HIV-1 capsid protein A92E in helical assembly (-13,12) 手法: EM (らせん対称) / 解像度: 4.9 Å 10229 6skn EMDB-10229, PDB-6skn: Structure of the native full-length HIV-1 capsid protein in helical assembly (-13,8) 手法: EM (らせん対称) / 解像度: 4.5 Å 10239 6slq EMDB-10239, PDB-6slq: Structure of the native full-length HIV-1 capsid protein A92E in helical assembly (-12,11) 手法: EM (らせん対称) / 解像度: 4.4 Å 10240 6slu EMDB-10240, PDB-6slu: Structure of the native full-length HIV-1 capsid protein A92E in helical assembly (-13,11) 手法: EM (らせん対称) / 解像度: 4.7 Å 10246 6smu EMDB-10246, PDB-6smu: Structure of the native full-length HIV-1 capsid protein in helical assembly (-13,12) 手法: EM (らせん対称) / 解像度: 5.0 Å 10738 6y9v EMDB-10738, PDB-6y9v: Structure of the native full-length HIV-1 capsid protein in complex with Cyclophilin A from helical assembly (-8,13) 手法: EM (単粒子) / 解像度: 6.9 Å 10739 6y9w EMDB-10739, PDB-6y9w: Structure of the native full-length HIV-1 capsid protein in complex with Cyclophilin A from helical assembly (-13,8) 手法: EM (単粒子) / 解像度: 4.1 Å 10740 6y9x EMDB-10740, PDB-6y9x: Structure of the native full-length HIV-1 capsid protein in complex with Cyclophilin A from helical assembly (-13,7) 手法: EM (単粒子) / 解像度: 4.4 Å 10741 6y9y EMDB-10741, PDB-6y9y: Structure of the native full-length HIV-1 capsid protein in complex with Cyclophilin A from helical assembly (-7,13) 手法: EM (単粒子) / 解像度: 6.1 Å 10742 6y9z EMDB-10742, PDB-6y9z: Structure of the native full-length HIV-1 capsid protein in complex with Cyclophilin A from helical assembly (-13,9) 手法: EM (単粒子) / 解像度: 4.8 Å 10816 6yj5 EMDB-10816, PDB-6yj5: Focused refinement cryo-EM structure of the yeast mitochondrial complex I sub-stoichiometric sulfur transferase subunit 手法: EM (単粒子) / 解像度: 3.5 Å 11176 6zdj EMDB-11176, PDB-6zdj: Structure of the native full-length HIV-1 capsid protein in complex with Cyclophilin A from helical assembly (-13,10) 手法: EM (単粒子) / 解像度: 5.8 Å
由来	human immunodeficiency virus 1 (ヒト免疫不全ウイルス) homo sapiens (ヒト) yarrowia lipolytica (酵母)
キーワード	VIRAL PROTEIN (ウイルスタンパク質) / HIV (ヒト免疫不全ウイルス) / capsid (カプシド) / hexamer (オリゴマー) / helical assembly / curvature (曲率) / TRANSFERASE (転移酵素) / NADH:Ubiquinone Oxidoreductase / sulfur transferase / sub-stoichiometric / complex I (NADH:ユビキノン還元酵素 (水素イオン輸送型))