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Structure paper

TitleThe myosin X motor is optimized for movement on actin bundles.
Journal, issue, pagesNat Commun, Vol. 7, Page 12456, Year 2016
Publish dateSep 1, 2016
AuthorsVirginie Ropars / Zhaohui Yang / Tatiana Isabet / Florian Blanc / Kaifeng Zhou / Tianming Lin / Xiaoyan Liu / Pascale Hissier / Frédéric Samazan / Béatrice Amigues / Eric D Yang / Hyokeun Park / Olena Pylypenko / Marco Cecchini / Charles V Sindelar / H Lee Sweeney / Anne Houdusse /
PubMed AbstractMyosin X has features not found in other myosins. Its structure must underlie its unique ability to generate filopodia, which are essential for neuritogenesis, wound healing, cancer metastasis and ...Myosin X has features not found in other myosins. Its structure must underlie its unique ability to generate filopodia, which are essential for neuritogenesis, wound healing, cancer metastasis and some pathogenic infections. By determining high-resolution structures of key components of this motor, and characterizing the in vitro behaviour of the native dimer, we identify the features that explain the myosin X dimer behaviour. Single-molecule studies demonstrate that a native myosin X dimer moves on actin bundles with higher velocities and takes larger steps than on single actin filaments. The largest steps on actin bundles are larger than previously reported for artificially dimerized myosin X constructs or any other myosin. Our model and kinetic data explain why these large steps and high velocities can only occur on bundled filaments. Thus, myosin X functions as an antiparallel dimer in cells with a unique geometry optimized for movement on actin bundles.
External linksNat Commun / PubMed:27580874 / PubMed Central
MethodsEM (helical sym.) / X-ray diffraction
Resolution1.8 - 9.1 Å
Structure data

8244

5kg8

EMDB-8244, PDB-5kg8:
Rigor myosin X co-complexed with an actin filament
Method: EM (helical sym.) / Resolution: 9.1 Å

PDB-5hmo:
myosin X motor activity
Method: X-RAY DIFFRACTION / Resolution: 3.493 Å

PDB-5hmp:
Myosin Vc pre-powerstroke state
Method: X-RAY DIFFRACTION / Resolution: 2.397 Å

PDB-5i0h:
Crystal structure of myosin X motor domain in pre-powerstroke state
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-5i0i:
Crystal structure of myosin X motor domain with 2IQ motifs in pre-powerstroke state
Method: X-RAY DIFFRACTION / Resolution: 3.15 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-VO4:
VANADATE ION / Vanadate

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

ChemComp-EDO:
1,2-ETHANEDIOL / Ethylene glycol

ChemComp-DMS:
DIMETHYL SULFOXIDE / DMSO, precipitant*YM / Dimethyl sulfoxide

ChemComp-HOH:
WATER / Water

ChemComp-BEF:
BERYLLIUM TRIFLUORIDE ION

ChemComp-SO4:
SULFATE ION / Sulfate

ChemComp-MPO:
3[N-MORPHOLINO]PROPANE SULFONIC ACID / pH buffer*YM / MOPS

Source
  • homo sapiens (human)
  • oryctolagus cuniculus (rabbit)
  • bos taurus (cattle)
KeywordsMOTOR PROTEIN / myosin / motor domain / molecular motor / pre-powerstroke state / motility / pre-powerstrocke state / myosin molecular motors cytoskeletal motility

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