EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of the ciliary tip central pair reveals the unique role of the microtubule-seam binding protein SPEF1.
ジャーナル・号・ページ	Curr Biol, Vol. 35, Issue 14, Page 3404-33417.e6, Year 2025
掲載日	2025年7月21日
著者	Thibault Legal / Ewa Joachimiak / Mireya Parra / Wang Peng / Amanda Tam / Corbin Black / Mayukh Guha / Chau Anh Nguyen / Avrin Ghanaeian / Melissa Valente-Paterno / Gary Brouhard / Jacek Gaertig / Dorota Wloga / Khanh Huy Bui /
PubMed 要旨	Motile cilia are unique organelles with the ability to move autonomously. The force generated by beating cilia propels cells and moves fluids. The ciliary skeleton is made of peripheral doublet ...Motile cilia are unique organelles with the ability to move autonomously. The force generated by beating cilia propels cells and moves fluids. The ciliary skeleton is made of peripheral doublet microtubules and a central pair (CP) with a distinct structure at the tip. In this study, we present a high-resolution structure of the CP in the ciliary tip of the ciliate Tetrahymena thermophila and identify several tip proteins that bind and form unique patterns on both microtubules of the tip CP. Two of those proteins that contain tubulin polymerization-promoting protein (TPPP)-like domains, TLP1 and TLP2, bind to high curvature regions of the microtubule. TLP2, which contains two TPPP-like domains, is an unusually long protein that wraps laterally around half a microtubule and forms the bridge between the two microtubules. Moreover, we found that the conserved protein SPEF1 binds to both microtubule seams and crosslinked the two microtubules. In vitro, human SPEF1 binds to the microtubule seam as visualized by cryoelectron tomography and subtomogram averaging. Single-molecule microtubule dynamics assays indicate that SPEF1 stabilizes microtubules in vitro. Together, these data show that the proteins in the tip CP maintain stable microtubule structures and play important roles in maintaining the integrity of the axoneme.
リンク	Curr Biol / PubMed:40651469 / PubMed Central
手法	EM (サブトモグラム平均) / EM (単粒子)
解像度	3.61 - 25.2 Å
構造データ	49760 9ntm EMDB-49760, PDB-9ntm: SPEF1 bound to 14-pf microtubule 手法: EM (サブトモグラム平均) / 解像度: 7.1 Å 49872 9nw3 EMDB-49872, PDB-9nw3: Ciliary tip central pair 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-49903: SPEF1 bound to 13-PF microtubule 手法: EM (サブトモグラム平均) / 解像度: 8.1 Å 70819 9ot2 EMDB-70819, PDB-9ot2: 13PF microtubule symmetry expansion 手法: EM (サブトモグラム平均) / 解像度: 5.8 Å EMDB-70821: Tip CP C1 Wild Type 手法: EM (サブトモグラム平均) / 解像度: 19.48 Å EMDB-70823: Tip CP C2 WT 手法: EM (サブトモグラム平均) / 解像度: 21.98 Å EMDB-70829: SPEF1 KO Tip CP C1 手法: EM (サブトモグラム平均) / 解像度: 25.2 Å EMDB-70830: SPEF1 KO Tip CP C2 手法: EM (サブトモグラム平均) / 解像度: 23.8 Å EMDB-71226: Refinement C1 PF2-3-4 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-71227: Refinement C1 PF13-1-2 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-71228: Refinement C1 PF4-5-6 手法: EM (単粒子) / 解像度: 4.36 Å EMDB-71229: Refinement C1 PF6-7-8 手法: EM (単粒子) / 解像度: 4.36 Å EMDB-71230: Refinement C1 PF8-9-10 手法: EM (単粒子) / 解像度: 4.19 Å EMDB-71231: Refinement C1 PF10-11-12-13 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-71232: Refinement C2 PF13-1-2 手法: EM (単粒子) / 解像度: 3.61 Å EMDB-71233: Refinement C2 PF2-3-4 手法: EM (単粒子) / 解像度: 3.68 Å EMDB-71234: Refinement C2 PF4-5-6 手法: EM (単粒子) / 解像度: 3.74 Å EMDB-71235: Refinement C2 PF6-7-8 手法: EM (単粒子) / 解像度: 3.73 Å EMDB-71236: Refinement C2 PF8-9-10 手法: EM (単粒子) / 解像度: 3.72 Å EMDB-71237: Refinement C2 PF10-11-12-13 手法: EM (単粒子) / 解像度: 3.74 Å
化合物	ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP, エネルギー貯蔵分子YM ChemComp-TA1: TAXOL / 薬剤*YM
由来	homo sapiens (ヒト) recombinant vesicular stomatitis indiana virus rvsv-g/gfp (ウイルス) bos taurus (ウシ) tetrahymena thermophila cu428 (真核生物) Tetrahymena thermophila (真核生物)
キーワード	PROTEIN BINDING / microtubules / spef1 / seam / cilia / central pair / STRUCTURAL PROTEIN / microtubule