Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Molecular architecture of the 90S small subunit pre-ribosome.
Journal, issue, pages	Elife, Vol. 6, Year 2017
Publish date	Feb 28, 2017
Authors	Qi Sun / Xing Zhu / Jia Qi / Weidong An / Pengfei Lan / Dan Tan / Rongchang Chen / Bing Wang / Sanduo Zheng / Cheng Zhang / Xining Chen / Wei Zhang / Jing Chen / Meng-Qiu Dong / Keqiong Ye /
PubMed Abstract	Eukaryotic small ribosomal subunits are first assembled into 90S pre-ribosomes. The complete 90S is a gigantic complex with a molecular mass of approximately five megadaltons. Here, we report the ...Eukaryotic small ribosomal subunits are first assembled into 90S pre-ribosomes. The complete 90S is a gigantic complex with a molecular mass of approximately five megadaltons. Here, we report the nearly complete architecture of 90S determined from three cryo-electron microscopy single particle reconstructions at 4.5 to 8.7 angstrom resolution. The majority of the density maps were modeled and assigned to specific RNA and protein components. The nascent ribosome is assembled into isolated native-like substructures that are stabilized by abundant assembly factors. The 5' external transcribed spacer and U3 snoRNA nucleate a large subcomplex that scaffolds the nascent ribosome. U3 binds four sites of pre-rRNA, including a novel site on helix 27 but not the 3' side of the central pseudoknot, and crucially organizes the 90S structure. The 90S model provides significant insight into the principle of small subunit assembly and the function of assembly factors.
External links	Elife / PubMed:28244370 / PubMed Central
Methods	EM (single particle) / X-ray diffraction
Resolution	1.9 - 8.7 Å
Structure data	6695 5wyj EMDB-6695, PDB-5wyj: Cryo-EM structure of the 90S small subunit pre-ribosome (Dhr1-depleted, Enp1-TAP, state 1) Method: EM (single particle) / Resolution: 8.7 Å 6696 5wyk EMDB-6696, PDB-5wyk: Cryo-EM structure of the 90S small subunit pre-ribosome (Mtr4-depleted, Enp1-TAP) Method: EM (single particle) / Resolution: 4.5 Å EMDB-6697: Cryo-EM structure of the 90S small subunit pre-ribosome (Noc4-TAP) Method: EM (single particle) / Resolution: 8.7 Å PDB-5wwn: Crystal structure of Tsr1 Method: X-RAY DIFFRACTION / Resolution: 2.805 Å PDB-5wwo: Crystal structure of Enp1 Method: X-RAY DIFFRACTION / Resolution: 2.4 Å PDB-5wxl: Crystal structure of the Rrs1 and Rpf2 complex Method: X-RAY DIFFRACTION / Resolution: 1.9 Å PDB-5wxm: Crystal structure of the Imp3 and Mpp10 complex Method: X-RAY DIFFRACTION / Resolution: 2.304 Å PDB-5wy3: Crystal structure of Chaetomium thermophilum Utp10 middle domain Method: X-RAY DIFFRACTION / Resolution: 3.2 Å PDB-5wyl: Crystal structure of Chaetomium thermophilum Utp10 N-terminal domain in complex with Utp17 C-terminal helices Method: X-RAY DIFFRACTION / Resolution: 2.638 Å
Chemicals	ChemComp-SO4: SULFATE ION ChemComp-HOH: WATER
Source	Saccharomyces cerevisiae (brewer's yeast) Baker's yeast (brewer's yeast) saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast) saccharomyces cerevisiae s288c (yeast) chaetomium thermophilum var. thermophilum dsm 1495 (fungus)
Keywords	RNA BINDING PROTEIN / ribosome biogenesis protein / component of 90S preribosome / NUCLEAR PROTEIN / protein complex / components of 90S preribosome / RIBOSOMAL PROTEIN / nucleolar protein / RIBOSOME / 90S / pre-ribosome / protein-RNA complex / RIBOSOMAL PROTEIN/NUCLEAR PROTEIN / RIBOSOMAL PROTEIN-NUCLEAR PROTEIN complex