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TitleStructure and assembly of the A-C linker connecting microtubule triplets in centrioles.
Journal, issue, pagesSci Adv, Vol. 11, Issue 41, Page eady3689, Year 2025
Publish dateOct 10, 2025
AuthorsBin Cai / Jingwei Xu / Erik H Collet / Ellen Aarts / Leo Luo / Alexander Leitner / Takashi Ishikawa / Pedro Beltrao / Chad G Pearson / Martin Pilhofer / Michal Wieczorek /
PubMed AbstractCentriole assembly involves the coordination of centriolar modules. One module is the A-C linker, an enigmatic protein assembly connecting the A-microtubule of one microtubule triplet to the C- ...Centriole assembly involves the coordination of centriolar modules. One module is the A-C linker, an enigmatic protein assembly connecting the A-microtubule of one microtubule triplet to the C-microtubule of the neighboring triplet. Here, we integrated biochemistry, multiscale cryo-electron microscopy, and AlphaFold modeling to investigate the architecture of the centriole. Using an improved centriole isolation method, we determined the structure of the A-C linker bound to microtubule triplets, which revealed how the A-C linker cross-links microtubules and integrates with the B-C junction. We found marked changes in the structure and composition of the A-C linker that correlate with the presence of other centriolar modules, including the pinhead, cartwheel, and inner scaffold. Our findings show that the A-C linker is a highly integrated component of the centriole whose polymorphism may orchestrate the assembly of spatially distinct centriolar modules, and provide a framework for dissecting the biology of centrioles.
External linksSci Adv / PubMed:41061066 / PubMed Central
MethodsEM (single particle) / EM (subtomogram averaging) / EM (tomography)
Resolution3.6 - 30.0 Å
Structure data

53467

9qzc

EMDB-53467: Composite map of proximal A-C linker of Tetrahymena centriole
PDB-9qzc: Proximal A-C linker of Tetrahymena centriole, one repeating unit
Method: EM (single particle) / Resolution: 3.8 Å

53471

9qzf

EMDB-53471: Composite map of proximal A-C linker of Tetrahymena centriole
PDB-9qzf: Proximal A-C linker of Tetrahymena centriole, six repeating units
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-53975: A-link map of Tetrahymena proximal A-C linker
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-53977: A-link and A-microtubule of Tetrahymena proximal A-C linker
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-53978: C-link of Tetrahymena proximal A-C linker
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-53979: A-microtubule associated with Tetrahymena proximal A-C linker
Method: EM (single particle) / Resolution: 6.4 Å

EMDB-53980: A05-A09 protofilaments of A-microtubule associated with Tetrahymena proximal A-C linker
Method: EM (single particle) / Resolution: 4.1 Å

EMDB-53981: A-B inner junction of Tetrahymena centriole triplet
Method: EM (single particle) / Resolution: 5.8 Å

EMDB-53982: B-C inner junction of Tetrahymena centriole triplet, focusing on POC1
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-53983: B-C inner junction of Tetrahymena centriole triplet, focusing on WD40 domain
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-53984: A-link and A-microtubule of Tetrahymena transitional A-C linker
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-53985: C-link of Tetrahymena transitional A-C linker
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-53986: C-link and C-microtubule of Tetrahymena transitional A-C linker
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-53987: Composite map of Tetrahymena transitional A-C linker
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-54008: Consensus refinement of Tetrahymena transitional A-C linker
Method: EM (single particle) / Resolution: 4.3 Å

EMDB-54049: Consensus refinement of Tetrahymena proximal A-C linker
Method: EM (single particle) / Resolution: 4.3 Å

EMDB-54239: Cryo sub-tomogram average of A-C linker from isolated Tetrahymena centrioles (Class01)
Method: EM (subtomogram averaging) / Resolution: 7.3 Å

EMDB-54240: Cryo sub-tomogram average of A-C linker from isolated Tetrahymena centrioles (Class02)
Method: EM (subtomogram averaging) / Resolution: 8.9 Å

EMDB-54241: Cryo sub-tomogram average of A-C linker from FIB-milled Tetrahymena cells
Method: EM (subtomogram averaging) / Resolution: 30.0 Å

EMDB-54242: Cryo-tomogram of FIB-milled Tetrahymena cell
Method: EM (tomography)

EMDB-54243: Cryo-tomogram of isolated centriole from Tetrahymena cells
Method: EM (tomography)

EMDB-54244: Cryo-tomogram of isolated oral apparatus from Tetrahymena cells
Method: EM (tomography)

Source
  • tetrahymena thermophila (eukaryote)
KeywordsSTRUCTURAL PROTEIN / centriole / basal body / centrosome

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