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- EMDB-53467: Composite map of proximal A-C linker of Tetrahymena centriole -

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Basic information

Entry
Database: EMDB / ID: EMD-53467
TitleComposite map of proximal A-C linker of Tetrahymena centriole
Map dataComposite map of Tetrahymena proximal A-C linker
Sample
  • Organelle or cellular component: proximal A-C linker of Tetrahymena thermophila basal body centriole
    • Protein or peptide: x 15 types
Keywordscentriole / basal body / centrosome / STRUCTURAL PROTEIN
Function / homology
Function and homology information


MWP complex / cell projection organization / centrosome cycle / microtubule organizing center / cilium assembly / centriole / centriolar satellite / ciliary basal body / centrosome / cytoplasm
Similarity search - Function
: / : / Rab-GTPase-TBC domain / Rab-GTPase-TBC domain superfamily / Rab-GTPase-TBC domain / TBC/rab GAP domain profile. / : / WD domain, G-beta repeat / G-protein beta WD-40 repeat / WD40 repeat, conserved site ...: / : / Rab-GTPase-TBC domain / Rab-GTPase-TBC domain superfamily / Rab-GTPase-TBC domain / TBC/rab GAP domain profile. / : / WD domain, G-beta repeat / G-protein beta WD-40 repeat / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily
Similarity search - Domain/homology
SWIM-type domain-containing protein / WD repeat WRAP73-like protein, putative / POC1 centriolar protein homolog / TBC1 domain family member 31 / Rab-GAP TBC domain-containing protein / Centrosomal protein, putative
Similarity search - Component
Biological speciesTetrahymena thermophila (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.8 Å
AuthorsCai B / Xu JW / Luo L / Aarts E / Leitner A / Ishikawa T / Beltro P / Pilhofer M / Wieczorek M
Funding support Switzerland, 3 items
OrganizationGrant numberCountry
ETH ZurichETH-22 22-1 Switzerland
Swiss National Science Foundation320030-231677 Switzerland
Swiss National Science FoundationIZLIZ3 200294 Switzerland
CitationJournal: Sci Adv / Year: 2025
Title: Structure and assembly of the A-C linker connecting microtubule triplets in centrioles.
Authors: Bin Cai / Jingwei Xu / Erik H Collet / Ellen Aarts / Leo Luo / Alexander Leitner / Takashi Ishikawa / Pedro Beltrao / Chad G Pearson / Martin Pilhofer / Michal Wieczorek /
Abstract: Centriole assembly involves the coordination of centriolar modules. One module is the A-C linker, an enigmatic protein assembly connecting the A-microtubule of one microtubule triplet to the C- ...Centriole assembly involves the coordination of centriolar modules. One module is the A-C linker, an enigmatic protein assembly connecting the A-microtubule of one microtubule triplet to the C-microtubule of the neighboring triplet. Here, we integrated biochemistry, multiscale cryo-electron microscopy, and AlphaFold modeling to investigate the architecture of the centriole. Using an improved centriole isolation method, we determined the structure of the A-C linker bound to microtubule triplets, which revealed how the A-C linker cross-links microtubules and integrates with the B-C junction. We found marked changes in the structure and composition of the A-C linker that correlate with the presence of other centriolar modules, including the pinhead, cartwheel, and inner scaffold. Our findings show that the A-C linker is a highly integrated component of the centriole whose polymorphism may orchestrate the assembly of spatially distinct centriolar modules, and provide a framework for dissecting the biology of centrioles.
History
DepositionApr 22, 2025-
Header (metadata) releaseSep 17, 2025-
Map releaseSep 17, 2025-
UpdateOct 22, 2025-
Current statusOct 22, 2025Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_53467.png

Downloads & links

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Map

FileDownload / File: emd_53467.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationComposite map of Tetrahymena proximal A-C linker
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.32 Å/pix.
x 480 pix.
= 631.2 Å		1.32 Å/pix.
x 480 pix.
= 631.2 Å		1.32 Å/pix.
x 480 pix.
= 631.2 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.315 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 5.0
Minimum - Maximum0.0 - 13.599174
Average (Standard dev.)0.22337514 (±1.0351868)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions480480480
Spacing480480480
CellA=B=C: 631.2 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : proximal A-C linker of Tetrahymena thermophila basal body centriole

EntireName: proximal A-C linker of Tetrahymena thermophila basal body centriole
Components
  • Organelle or cellular component: proximal A-C linker of Tetrahymena thermophila basal body centriole
    • Protein or peptide: WD repeat WRAP73-like protein, putative
    • Protein or peptide: Centrosomal protein, putative
    • Protein or peptide: Rab-GAP TBC domain-containing protein
    • Protein or peptide: TBC1 domain family member 31
    • Protein or peptide: POC1 centriolar protein homolog
    • Protein or peptide: Unknown Protein Chain
    • Protein or peptide: Unknown Protein Chain
    • Protein or peptide: SWIM-type domain-containing protein
    • Protein or peptide: Unknown Protein Chain
    • Protein or peptide: Unknown Protein Chain
    • Protein or peptide: Unknown Protein Chain
    • Protein or peptide: Unknown Protein Chain
    • Protein or peptide: Unknown Protein Chain
    • Protein or peptide: Unknown Protein Chain
    • Protein or peptide: Unknown Protein Chain

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Supramolecule #1: proximal A-C linker of Tetrahymena thermophila basal body centriole

SupramoleculeName: proximal A-C linker of Tetrahymena thermophila basal body centriole
type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Tetrahymena thermophila (eukaryote)

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Macromolecule #1: WD repeat WRAP73-like protein, putative

MacromoleculeName: WD repeat WRAP73-like protein, putative / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 51.977465 KDa
SequenceString: MQFSNIIQGA CSAIFSQDSS LIAISNGSKI LIKDINQELI NRNVYSFQDI ITHMEFSPDN QLILVVQSNS NTVEVKNFND EQWICKIVD QACGLLHAVW APDSRSIITF SDFQMKATIY SLTDKQQYLI KNPKYTDKGL SFSSDGKFMA LAERRDIKDY I GIYYTKDW ...String:
MQFSNIIQGA CSAIFSQDSS LIAISNGSKI LIKDINQELI NRNVYSFQDI ITHMEFSPDN QLILVVQSNS NTVEVKNFND EQWICKIVD QACGLLHAVW APDSRSIITF SDFQMKATIY SLTDKQQYLI KNPKYTDKGL SFSSDGKFMA LAERRDIKDY I GIYYTKDW KLVNHFQVDT MDLVDIQWSK DDGSILVWDS SINYKFLVYH PSNGLIQRIQ PYEYALGIKC VELSNTPNFV AI GSYDEKI RLVNTLTWKI ITELEHKANI KEYSNAVVYR EQNSTLLNAQ HLVDRTTTQF VIDESINKLP GAKQSTDKSQ QSG VTDVTF SFNANYIASK SASFPNAVFI WEVSLLELRY IILLQNPVKG FLWSHNSNNL IIYAGSNKIY FWSLDGASVC ELPQ YGKQF SVNKVLWNKN SKCLVLIDRR DAIIGYPPID LEYNEEHEEE QREEYEN

UniProtKB: WD repeat WRAP73-like protein, putative

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Macromolecule #2: Centrosomal protein, putative

MacromoleculeName: Centrosomal protein, putative / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 44.594297 KDa
SequenceString: MRNSLFNERK YLLLRKKLDE LHYTQPLQIE SAELVDKLLN DLLKTSEGFQ KLKNKNQELE QQLCIIEKSL NPLKAENQKL MRENNNLQS EIILSSENAQ QKYNLLTVRL KQLQTEAQDL KFVLQKSEER CLKLQDENSA LKNKLDTISA KVYLPSCEKG F EDLPKSYV ...String:
MRNSLFNERK YLLLRKKLDE LHYTQPLQIE SAELVDKLLN DLLKTSEGFQ KLKNKNQELE QQLCIIEKSL NPLKAENQKL MRENNNLQS EIILSSENAQ QKYNLLTVRL KQLQTEAQDL KFVLQKSEER CLKLQDENSA LKNKLDTISA KVYLPSCEKG F EDLPKSYV NSNYKSLTNM SIARDNREGF NVLPQDNSNQ KNSNSSKEYD IQRLQNISDS KQNYQDGYSN LISTIKELQE QL RNCKEQV HTQEAQLSYL KMQVSNRDGE INRLKTRLDV GGINMVKLVQ DYNYMQQNDK IKLLNHQIDY LNKENTEMDS ILR SKPYIA LSQANVKLLA ENSSYLEKLR KTEEILEEYE RRFQIQQQSA KYTKQLSQNA LVDN

UniProtKB: Centrosomal protein, putative

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Macromolecule #3: Rab-GAP TBC domain-containing protein

MacromoleculeName: Rab-GAP TBC domain-containing protein / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 110.098609 KDa
SequenceString: MKISQQQKLS LVNRTGKLEA KKESGQDGLC DVINFKTLKK GNWHSFKLQF DSKGATYGCL DQAGNVTLFY LDKNRYTNVD VQVDSSNLT FIEKPTGYLA VSNADCSISI YSILSGKLIQ QLKWHTFKIK KLQVNHMSGY LLSVSSDNCT IWNINTYERR K SFFVKNTR ...String:
MKISQQQKLS LVNRTGKLEA KKESGQDGLC DVINFKTLKK GNWHSFKLQF DSKGATYGCL DQAGNVTLFY LDKNRYTNVD VQVDSSNLT FIEKPTGYLA VSNADCSISI YSILSGKLIQ QLKWHTFKIK KLQVNHMSGY LLSVSSDNCT IWNINTYERR K SFFVKNTR YAQGCLTPAG EHLITLSQNG LVECHNTDTL KIEKKEEIGL KLKQAHLDIS AEGVYIAICG DSNYCLIGRL DC QQGFDFE LYELPNNIQS VKKIKFIRNS LLTLLGSDEK IYVLSAENNM EIVLCFELPS RKGIVDYDLN IDAKYLVVIS TLG EKYVYD FHQLIANQKK HQQDVTMKKG SFSRQTLTRM DLDELKQTLF YQTKENQMKY ANSKKQKHEG KINGETNMIM LDRV GGATP EVEIFVNGKR QDQYDIQNQK SSFLDREKLR EFLSRFGYFP EKHRVVVWNY LLKLPSNYEA FDNLARKGLH PNYLN LKST FPIQSDKLYY RTQRILSCLA HYCPVFAQLD YMPAFVFPFV KLLGTNEAFC FEVILSFLVQ WGQFFFEFAP NPPVSY LKA FMELLEYHEP QLHAQLNQNI PHTQLFNTIW SSLQVVLTDV LKREDWLCFI DFLVAHSEKP DLLLHILVAY FTYFKRH II SFTTGSQLLR FFQQDHQIRI QNLLQDALEY RNRTPPRIPQ AVVLQDNLPL TSEQYPIFNF YPKFSLDQHK EIREQIIQ E ELKATEKKRT LKELNTLQQQ LNDLEKLYEE KQDALMRHER ERREIITYEQ EMRYSKLVDA ERQVREERLQ QMKKLEEES RKAIQIAENS RKADLDFIDQ ELQKKRANDN KFIASKMEEE AILNLELKTA GRINEVLNTK TKDQNIQSIT LQAEIAQKRL DMKYKQMEQ QIDQQIEEEN IRQDHILQRK ALQREIEDDV SKQREIKGQL LAEEFERE

UniProtKB: Rab-GAP TBC domain-containing protein

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Macromolecule #4: TBC1 domain family member 31

MacromoleculeName: TBC1 domain family member 31 / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 141.643859 KDa
SequenceString: MNNIHTSTHV STYFNEALVD RIDTQKYLKS NGKIKKVVSD PDNHYTAAID DSGNITVIVH KLNRFVSIKP SSPATHLVFI RNGNLLVAQ QNGHISQYLA SNGKLTSVIK HHNSKLLALE YNPVTKQILT IANDQCTLWD AQSFNKIKTL FAKASYFATG S LTPNGQSL ...String:
MNNIHTSTHV STYFNEALVD RIDTQKYLKS NGKIKKVVSD PDNHYTAAID DSGNITVIVH KLNRFVSIKP SSPATHLVFI RNGNLLVAQ QNGHISQYLA SNGKLTSVIK HHNSKLLALE YNPVTKQILT IANDQCTLWD AQSFNKIKTL FAKASYFATG S LTPNGQSL ITSFQDGNVY VWNLNNFEVT AKSEVEENMK NQKISVSADS SRFVIGGSTA YLYYCDISHS PLCISSFALP SG STSVKKI KFTHGNYLGI ITDNQVFYAI DSSTNQIALE FRLPKNKGIT DFDINPNNLE ILIVSGNGEI FKYDLQKLFI MEM NQQQRL LERGIESSLV YNVLKPGNQN HSLKFESHVD FVSHGKENVI INGESKLVND PDDLMKANTQ RETNIIQLCT FPKH TLEKM FKSALINVNS LPNMSVHKLR QFLQKHKVYP DKHRAFIYKQ LLELPLNVDN FATLVQKGLY PSFKDLYRKY PISDS KLYQ KMQTILSCLA NFCDLFGESD QFIQIAFPFV KLFGQEELLA FEIVLCFAHQ ILQNYFVDFP HPPLDLLQNI DEIIKY NDP ILHQHLKQHN FKFVDYIWPF LSGFFTSALD KESWLQLIDY LITYNDTPEL FMFITAAFII YFKHPLMKLK SKDELEI FI SRQNPVSVNH IIESGFSLLK RTPIDKIFVK FSKILPLSKT SYNQFTNYNV FNVAHSRLTR QQETEAEAKL EESKHKIR Q MQSLTDQILS VEQRYKEKQE NLIRSEQIRR EEVIYNAEQK LSRQMKLEES IKQTRLQQLN KIQTLTRANL QAQEELRQK ELDLLEAELQ YQQQADGQQM QNRKEEIQIL EMEAQAAARL RQVMELREKE DRQRMVRMDM DIHRQQEEDR DNALIQQWRV EDTEMRLRR ETLKQKKLQD LYKYEEENEK KLIEAKMLET EILKEQQMRD IERERKLRQI AEQELDQNER YAEYVKQLEQ E QLKTQIHK LQETLNHHRQ KALNFSQARL NQAEEEKNRL LNEIQIHKNM LSAKQKTLQT GEIESQIAQK RREAEQSIIE HE KKLQSIL LDIENEKRLM YELQSQILQR EKDIKEQEGF YDVLKENEQR AINENRYQTA LQQQRFEEDL NRQTMQKSYQ QQQ RLQEQM QLREQQLIQE TDQMRETIQQ ENMKRLEEAV NNNTLLRNTN SNNQSQDYSP ANNFQRNHYK MPSEINEEEG EQDE EQ

UniProtKB: TBC1 domain family member 31

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Macromolecule #5: POC1 centriolar protein homolog

MacromoleculeName: POC1 centriolar protein homolog / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 71.408133 KDa
SequenceString: MKKTTIASSA VASQKQTQSK LGNTSSKITE QQTTHDPALS RSFRGHEDII TCADFDPKMR QVASCSKDSI VYVWNFKPQQ RPFKFVGHK GAVYCVKYSP DGETIASCGQ DRQIRLWQNT VQSKCSIIKA HCGAIRSMSF SADGGYLLSS SDDKTLKLWR L QDKKFMCS ...String:
MKKTTIASSA VASQKQTQSK LGNTSSKITE QQTTHDPALS RSFRGHEDII TCADFDPKMR QVASCSKDSI VYVWNFKPQQ RPFKFVGHK GAVYCVKYSP DGETIASCGQ DRQIRLWQNT VQSKCSIIKA HCGAIRSMSF SADGGYLLSS SDDKTLKLWR L QDKKFMCS FAGHKNWVRS GVISPDMRLV ASGSDDKTVM LWDLNFQKIV SKYSTLDDNV KISQINSYSD HMDTVSQVLF HP DGTCLIS SSFDHKIKIT DIRSNKLIQH YNAHDAQVNS ISIHPTGYFL ASAGSDSKIK IWDLRQGRQI YTLYSNDKDI TTV QFNQSG DYFATGGSQN LCMVWKTNFD QNIQNIEQVQ RSTVRQNDVE YSSGGLYAQT LFKTNKTASL SPSKKTISII PPQQ LQDTF NNRGDDELNN GRVHENKQSN SPTFSQQQQQ QQLNLSENQY PGLEIVGNQF NLQQSTLSNN NNQNNLIKSQ NDSIV SNKN AIPFHPSSEF QQSAVQQQFS QQPKLVVNNG TATYPSNFVP KVEEENIMTE AIAASMQQIY SRLDTMFQYM TNLENR VVQ SEQLQKSFVS QNHQNTLDPK QQTMNFGYSN IQDHSLSASL NDRNVVNIFQ AGALANTGSI EASLINKQQE E

UniProtKB: POC1 centriolar protein homolog

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Macromolecule #6: Unknown Protein Chain

MacromoleculeName: Unknown Protein Chain / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 2.74137 KDa
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

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Macromolecule #7: Unknown Protein Chain

MacromoleculeName: Unknown Protein Chain / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 13.039064 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)

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Macromolecule #8: SWIM-type domain-containing protein

MacromoleculeName: SWIM-type domain-containing protein / type: protein_or_peptide / ID: 8 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 26.274373 KDa
SequenceString: MFSKAPRPAS CKPLSSKEVI SIQKGATDLG KALFYAIKHQ DYKHASELVA LGAPIERCYE VEKDVYRTTC SLLVGMICTA RIAKGHREI SYRIPDRILD TFIQLGVDLR CHCSNKFEHR CPLFVALKIL NVQNEQELFK DKNQIVQKLI INGATIKNHI Q PHKTFHNI ...String:
MFSKAPRPAS CKPLSSKEVI SIQKGATDLG KALFYAIKHQ DYKHASELVA LGAPIERCYE VEKDVYRTTC SLLVGMICTA RIAKGHREI SYRIPDRILD TFIQLGVDLR CHCSNKFEHR CPLFVALKIL NVQNEQELFK DKNQIVQKLI INGATIKNHI Q PHKTFHNI PHFDIEGIRS YLISKSQLAS VKKSGSLQTA TSNYHDNCKN PLPYKHNSEF SKTGYNFTFQ YK

UniProtKB: SWIM-type domain-containing protein

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Macromolecule #9: Unknown Protein Chain

MacromoleculeName: Unknown Protein Chain / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 12.868854 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #10: Unknown Protein Chain

MacromoleculeName: Unknown Protein Chain / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 13.975214 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)

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Macromolecule #11: Unknown Protein Chain

MacromoleculeName: Unknown Protein Chain / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 14.230525 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

+
Macromolecule #12: Unknown Protein Chain

MacromoleculeName: Unknown Protein Chain / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 13.719902 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)

+
Macromolecule #13: Unknown Protein Chain

MacromoleculeName: Unknown Protein Chain / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 8.954028 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)

+
Macromolecule #14: Unknown Protein Chain

MacromoleculeName: Unknown Protein Chain / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 10.826337 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)

+
Macromolecule #15: Unknown Protein Chain

MacromoleculeName: Unknown Protein Chain / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 8.698714 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statefilament

-
Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE-PROPANE

-
Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 35.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 0.8 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: OTHER
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.8 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 155485
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

+
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