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- EMDB-53982: B-C inner junction of Tetrahymena centriole triplet, focusing on POC1 -

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Basic information

Entry
Database: EMDB / ID: EMD-53982
TitleB-C inner junction of Tetrahymena centriole triplet, focusing on POC1
Map dataB-C inner junction of Tetrahymena centriole triplet focusing on POC1, B-factor sharpened using relion_posprocess, original pixel size 1.34
Sample
  • Organelle or cellular component: proximal A-C linker of Tetrahymena thermophila basal body centriole
Keywordscentriole / basal body / centrosome / STRUCTURAL PROTEIN
Biological speciesTetrahymena thermophila (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.8 Å
AuthorsCai B / Xu JW / Luo L / Aarts E / Leitner A / Ishikawa T / Beltro P / Pilhofer M / Wieczorek M
Funding support Switzerland, 3 items
OrganizationGrant numberCountry
ETH ZurichETH-22 22-1 Switzerland
Swiss National Science Foundation320030-231677 Switzerland
Swiss National Science FoundationIZLIZ3 200294 Switzerland
CitationJournal: To Be Published
Title: Structure and assembly of the A-C linker connecting microtubule triplets in centrioles
Authors: Cai B / Xu JW / Luo L / Aarts E / Leitner A / Ishikawa T / Beltro P / Pilhofer M / Wieczorek M
History
DepositionJun 10, 2025-
Header (metadata) releaseSep 17, 2025-
Map releaseSep 17, 2025-
UpdateSep 17, 2025-
Current statusSep 17, 2025Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_53982.png

Downloads & links

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Map

FileDownload / File: emd_53982.map.gz / Format: CCP4 / Size: 30.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationB-C inner junction of Tetrahymena centriole triplet focusing on POC1, B-factor sharpened using relion_posprocess, original pixel size 1.34
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.34 Å/pix.
x 200 pix.
= 268. Å		1.34 Å/pix.
x 200 pix.
= 268. Å		1.34 Å/pix.
x 200 pix.
= 268. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.34 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.01
Minimum - Maximum-0.0682102 - 0.083160624
Average (Standard dev.)0.000027904427 (±0.0028522043)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions200200200
Spacing200200200
CellA=B=C: 268.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: B-C inner junction of Tetrahymena centriole triplet focusing...

Fileemd_53982_additional_1.map
AnnotationB-C inner junction of Tetrahymena centriole triplet focusing on POC1, B-factor sharpened using relion_posprocess, pixel size corrected to 1.315
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: B-C inner junction of Tetrahymena centriole triplet focusing...

Fileemd_53982_additional_2.map
AnnotationB-C inner junction of Tetrahymena centriole triplet focusing on POC1, processed using EMReady, pixel size corrected to 1.315
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_53982_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_53982_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : proximal A-C linker of Tetrahymena thermophila basal body centriole

EntireName: proximal A-C linker of Tetrahymena thermophila basal body centriole
Components
  • Organelle or cellular component: proximal A-C linker of Tetrahymena thermophila basal body centriole

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Supramolecule #1: proximal A-C linker of Tetrahymena thermophila basal body centriole

SupramoleculeName: proximal A-C linker of Tetrahymena thermophila basal body centriole
type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: #1-#27
Source (natural)Organism: Tetrahymena thermophila (eukaryote)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statefilament

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE-PROPANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 35.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 0.8 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: OTHER
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 231875
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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