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| Title | Single-cell visual proteomics of a minimal bacterium reveals structural coordination of gene expression machineries. |
|---|---|
| Journal, issue, pages | Cell, Year 2026 |
| Publish date | Sep 25, 2026 |
 Authors | Joseph M Dobbs / Rasmus K Jensen / Julia Mahamid /  |
| PubMed Abstract | Translation is a central process in gene expression. Its regulation is complex, depends on factors that include cell state and the subcellular environment, and is subject to modulation via crosstalk ...Translation is a central process in gene expression. Its regulation is complex, depends on factors that include cell state and the subcellular environment, and is subject to modulation via crosstalk to processes such as transcription or translocation. Here, we used cryo-electron tomography of native and antibiotic-perturbed Mycoplasma pneumoniae cells to resolve 140 maps that recapitulate bacterial translation during the initiation, elongation, and recycling phases. We visualized multiple transcription-translation complexes, allowing us to propose a threading-based translation reinitiation mechanism and to provide structural evidence for a long-hypothesized supercomplex that coordinates transcription, translation, and membrane attachment. We resolved abundant membrane-associated large ribosomal subunits and suggest that dissociation from membranes depends on the conditional initiation of new translation, consistent with a potentially conserved mechanism in mammalian cells. This work visualizes the multilayered control of bacterial translation and demonstrates the power of in-cell structural biology to investigate regulatory circuits in gene expression. |
 External links | Cell / PubMed:42790420 |
| Methods | EM (subtomogram averaging) |
| Resolution | 3.6 - 28.9 Å |
| Structure data |  EMDB-53075: Consensus map of M. pneumoniae 30S small ribosomal subunit in native cells  EMDB-53309: Consensus map of M. pneumoniae 50S large ribosomal subunit in native cells  EMDB-53611: Consensus map of M. pneumoniae 70S ribosome in native cells EMDB-53612: Map of M. pneumoniae 30S empty/factorless in native cells EMDB-53613: Map of M. pneumoniae 30S IF1/3 in native cells EMDB-53614: Map of M. pneumoniae 30S IF1/3 tRNA in native cells  EMDB-53615: Map of M. pneumoniae 30S IF2 (all, flexible) in native cells  EMDB-53616: Map of M. pneumoniae 30S IF2 (all, stable) in native cells EMDB-53617: Map of M. pneumoniae 30S IF1/2/3 in native cells EMDB-53618: Map of M. pneumoniae 30S IF3 in native cells EMDB-53620: Map of M. pneumoniae 30S IF1/2/3 tRNA in native cells  EMDB-53622: Map of M. pneumoniae 30S late biogenesis in native cells EMDB-53623: Map of M. pneumoniae 30S P-tRNA in native cells EMDB-53624: Map of M. pneumoniae 30S iT-TC (flexible) in native cells  EMDB-53625: Map of M. pneumoniae 30S iT-TC (stable) in native cells  EMDB-53626: Map of M. pneumoniae 50S empty/factorless in native cells EMDB-53627: Map of M. pneumoniae 50S RRF, EF-G in native cells  EMDB-53628: Map of M. pneumoniae 50S late/inactive in native cells EMDB-53630: Map of M. pneumoniae 50S membrane complex in native cells  EMDB-53631: Map of M. pneumoniae 50S mid biogenesis in native cells  EMDB-53637: Map of M. pneumoniae 50S RRF in native cells  EMDB-53641: Map of M. pneumoniae 50S trigger factor in native cells  EMDB-53648: Map of M. pneumoniae 70S A,P,E in native cells  EMDB-53649: Map of M. pneumoniae 70S A,P in native cells  EMDB-53652: Map of M. pneumoniae 70S A,P,E/EOUT in native cells  EMDB-53653: Map of M. pneumoniae 70S A/P,P/E in native cells  EMDB-53654: Map of M. pneumoniae 70S A, P, EOUT in native cells  EMDB-53657: Map of M. pneumoniae 70S A*, P/E in native cells  EMDB-53658: Map of M. pneumoniae 70S A/P,P/E, EF-G in native cells  EMDB-53659: Map of M. pneumoniae 70S ap/P,pe/E in native cells  EMDB-53660: Map of M. pneumoniae 70S A*,P/E, EF-G in native cells  EMDB-53661: Map of M. pneumoniae 70S EF-Tu A/T,P in native cells  EMDB-53662: Map of M. pneumoniae 70S EF-Tu A/T,P,E in native cells  EMDB-53663: Map of M. pneumoniae 70S S4LSU in native cells EMDB-53664: Map of M. pneumoniae 70S membrane complex in native cells  EMDB-53665: Map of M. pneumoniae 70S P in native cells  EMDB-53666: Map of M. pneumoniae 70S P,E in native cells  EMDB-53667: Map of M. pneumoniae 70S P, EOUT in native cells EMDB-53668: Map of M. pneumoniae 70S eT-TC (collided) in native cells  EMDB-53669: Map of M. pneumoniae 70S P/E in native cells EMDB-53670: Map of M. pneumoniae 70S eT-TC (flexible) in native cells EMDB-53671: Map of M. pneumoniae 70S eT-TC (stable) in native cells  EMDB-53672: Map of M. pneumoniae 70S trigger factor in native cells  EMDB-53674: Map of M. pneumoniae 70S transertion-like (collided RNAP) in native cells  EMDB-53675: Map of M. pneumoniae 70S transertion-like (flexible RNAP) in native cells EMDB-53676: Map of M. pneumoniae 70S transertion-like (stable RNAP) in native cells  EMDB-53677: Map of M. pneumoniae 30S iT-TC (flexible RNAP recentered subset) in native cells  EMDB-53678: Map of M. pneumoniae 70S eT-TC (flexible RNAP recentered subset) in native cells  EMDB-53688: Consensus map of M. pneumoniae 30S small ribosomal subunit in pseudouridimycin-treated cells  EMDB-53689: Consensus map of M. pneumoniae 50S large ribosomal subunit in pseudouridimycin-treated cells  EMDB-53690: Consensus map of M. pneumoniae 70S ribosome in pseudouridimycin-treated cells  EMDB-53693: Map of M. pneumoniae 30S empty/factorless in pseudouridimycin-treated cells  EMDB-53694: Map of M. pneumoniae 30S IF1/3 in pseudouridimycin-treated cells  EMDB-53695: Map of M. pneumoniae 30S IF1/3 tRNA in pseudouridimycin-treated cells  EMDB-53696: Map of M. pneumoniae 30S IF1/2/3 in pseudouridimycin-treated cells  EMDB-53697: Map of M. pneumoniae 30S IF1/2/3 tRNA in pseudouridimycin-treated cells  EMDB-53698: Map of M. pneumoniae 30S iT-TC (flexible) in pseudouridimycin-treated cells EMDB-53699: Map of M. pneumoniae 30S iT-TC (stable) in pseudouridimycin-treated cells  EMDB-53700: Map of M. pneumoniae 50S RRF, EF-G in pseudouridimycin-treated cells  EMDB-53701: Map of M. pneumoniae 50S empty/factorless in pseudouridimycin-treated cells  EMDB-53702: Map of M. pneumoniae 50S S4LSU in pseudouridimycin-treated cells  EMDB-53703: Map of M. pneumoniae 50S late/inactive in pseudouridimycin-treated cells  EMDB-53704: Map of M. pneumoniae 50S membrane complex in pseudouridimycin-treated cells  EMDB-53705: Map of M. pneumoniae 50S RRF in pseudouridimycin-treated cells  EMDB-53706: Map of M. pneumoniae 50S trigger factor in pseudouridimycin-treated cells  EMDB-53707: Map of M. pneumoniae 70S A,P in pseudouridimycin-treated cells  EMDB-53708: Map of M. pneumoniae 70S A/P,P/E in pseudouridimycin-treated cells  EMDB-53709: Map of M. pneumoniae 70S A*,P/E in pseudouridimycin-treated cells  EMDB-53713: Map of M. pneumoniae 70S A/P,P/E, EF-G in pseudouridimycin-treated cells  EMDB-53714: Map of M. pneumoniae 70S dome complex in pseudouridimycin-treated cells  EMDB-53715: Map of M. pneumoniae 70S A*,P/E, EF-G in pseudouridimycin-treated cells  EMDB-53719: Map of M. pneumoniae 70S EF-Tu A/T,P in pseudouridimycin-treated cells  EMDB-53726: Map of M. pneumoniae 70S EF-Tu A/T,P,E in pseudouridimycin-treated cells  EMDB-53730: Map of M. pneumoniae 70S S4LSU in pseudouridimycin-treated cells  EMDB-53733: Map of M. pneumoniae 70S membrane complex (no RNAP) in pseudouridimycin-treated cells  EMDB-53734: Map of M. pneumoniae 70S transertion-like (collided RNAP) in pseudouridimycin-treated cells  EMDB-53737: Map of M. pneumoniae 70S P,E in pseudouridimycin-treated cells  EMDB-53738: Map of M. pneumoniae 70S P in pseudouridimycin-treated cells  EMDB-53748: Map of M. pneumoniae 70S eT-TC (collided) in pseudouridimycin-treated cells  EMDB-53749: Map of M. pneumoniae 70S trigger factor in pseudouridimycin-treated cells  EMDB-53752: Consensus map of M. pneumoniae 30S small ribosomal subunit in fidaxomicin-treated cells  EMDB-53753: Consensus map of M. pneumoniae 70S ribosome in fidaxomicin-treated cells  EMDB-53754: Consensus map of M. pneumoniae 50S large ribosomal subunit in fidaxomicin-treated cells  EMDB-53755: Map of M. pneumoniae 30S empty/factorless in fidaxomicin-treated cells  EMDB-53756: Map of M. pneumoniae 30S IF1/3 in fidaxomicin-treated cells  EMDB-53757: Map of M. pneumoniae 30S IF1/3 tRNA in fidaxomicin-treated cells  EMDB-53758: Map of M. pneumoniae 30S IF3 in fidaxomicin-treated cells  EMDB-53759: Map of M. pneumoniae 30S late biogenesis in fidaxomicin-treated cells  EMDB-53760: Map of M. pneumoniae 30S iT-TC (flexible) in fidaxomicin-treated cells EMDB-53761: Map of M. pneumoniae 30S iT-TC (stable) in fidaxomicin-treated cells  EMDB-53762: Map of M. pneumoniae 50S EF-Tu A/T? in fidaxomicin-treated cells  EMDB-53763: Map of M. pneumoniae 50S empty/factorless in fidaxomicin-treated cells  EMDB-53764: Map of M. pneumoniae 50S membrane complex in fidaxomicin-treated cells  EMDB-53765: Map of M. pneumoniae 50S PTC flipped out in fidaxomicin-treated cells  EMDB-53766: Map of M. pneumoniae 50S RRF in fidaxomicin-treated cells  EMDB-53767: Map of M. pneumoniae 50S trigger factor in fidaxomicin-treated cells  EMDB-53768: Map of M. pneumoniae 70S A,P in fidaxomicin-treated cells  EMDB-53769: Map of M. pneumoniae 70S A/P,P/E in fidaxomicin-treated cells  EMDB-53770: Map of M. pneumoniae 70S A*,P/E in fidaxomicin-treated cells  EMDB-53771: Map of M. pneumoniae 70S EF-Tu A/T in fidaxomicin-treated cells  EMDB-53772: Map of M. pneumoniae 70S EF-Tu A/T,E in fidaxomicin-treated cells  EMDB-53773: Map of M. pneumoniae 70S EF-Tu A/T,P in fidaxomicin-treated cells  EMDB-53774: Map of M. pneumoniae 70S EF-Tu A/T,P,E in fidaxomicin-treated cells  EMDB-53775: Map of M. pneumoniae 70S P,E in fidaxomicin-treated cells  EMDB-53776: Map of M. pneumoniae 70S membrane complex in fidaxomicin-treated cells  EMDB-53777: Map of M. pneumoniae 70S P/E in fidaxomicin-treated cells  EMDB-53778: Map of M. pneumoniae 70S trigger factor in fidaxomicin-treated cells  EMDB-53779: Map of M. pneumoniae 70S tmRNA-SmpB,P,E in fidaxomicin-treated cells  EMDB-53780: Consensus map of M. pneumoniae 30S small ribosomal subunit in chloramphenicol-treated cells  EMDB-53781: Consensus map of M. pneumoniae 50S large ribosomal subunit in chloramphenicol-treated cells  EMDB-53786: Consensus map of M. pneumoniae 70S ribosome in chloramphenicol-treated cells  EMDB-53788: Map of M. pneumoniae 30S IF1/3 tRNA in chloramphenicol-treated cells  EMDB-53789: Map of M. pneumoniae 30S IF2 (all, shifted) in chloramphenicol-treated cells  EMDB-53797: Map of M. pneumoniae 30S IF3 in chloramphenicol-treated cells  EMDB-53798: Map of M. pneumoniae 30S IF2 tRNA in chloramphenicol-treated cells  EMDB-53811: Map of M. pneumoniae 30S IF1/2/3 tRNA in chloramphenicol-treated cells  EMDB-53812: Map of M. pneumoniae 30S P-tRNA in chloramphenicol-treated cells  EMDB-53813: Map of M. pneumoniae 30S iT-TC (flexible) in chloramphenicol-treated cells  EMDB-53814: Map of M. pneumoniae 50S empty/factorless in chloramphenicol-treated cells  EMDB-53815: Map of M. pneumoniae 50S S4LSU in chloramphenicol-treated cells  EMDB-53816: Map of M. pneumoniae 50S membrane complex in chloramphenicol-treated cells  EMDB-53817: Map of M. pneumoniae 50S late/inactive in chloramphenicol-treated cells  EMDB-53818: Map of M. pneumoniae 50S PTC flipped out, P in chloramphenicol-treated cells  EMDB-53819: Map of M. pneumoniae 50S trigger factor in chloramphenicol-treated cells  EMDB-53822: Map of M. pneumoniae 50S EF-Tu A/T, tmRNA-SmpB, E in chloramphenicol-treated cells  EMDB-53823: Map of M. pneumoniae 70S A,P in chloramphenicol-treated cells  EMDB-53824: Map of M. pneumoniae 70S a,P in chloramphenicol-treated cells  EMDB-53825: Map of M. pneumoniae 70S A,P,E in chloramphenicol-treated cells  EMDB-53826: Map of M. pneumoniae 70S A,P,EOUT in chloramphenicol-treated cells  EMDB-53830: Map of M. pneumoniae 70S A*,P/E in chloramphenicol-treated cells  EMDB-53833: Map of M. pneumoniae 70S dome complex in chloramphenicol-treated cells  EMDB-53837: Map of M. pneumoniae 70S EF-Tu A/T,P in chloramphenicol-treated cells  EMDB-53838: Map of M. pneumoniae 70S EF-Tu A/T,P,E in chloramphenicol-treated cells  EMDB-53839: Map of M. pneumoniae 70S S4LSU in chloramphenicol-treated cells  EMDB-53840: Map of M. pneumoniae 70S trigger factor in chloramphenicol-treated cells  EMDB-53841: Map of M. pneumoniae 70S membrane complex in chloramphenicol-treated cells  EMDB-53845: Map of M. pneumoniae 30S iT-TC (flexible RNAP recentered subset) in pseudouridimycin-treated cells  EMDB-53846: Map of H. sapiens 60S large ribosomal subunit with Sec61-TRAP-OSTA translocon  EMDB-53849: Map of E. coli 50S membrane complex with SecYEG |
| Chemicals |  ChemComp-ZN: |
| Source |
|
 Keywords | TRANSLATION / 30S / ribosome / 50S / membrane / 70S / RNAP |
mycoplasmoides pneumoniae m129 (bacteria)
Homo sapiens (human)
Escherichia coli (E. coli)