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- PDB-9sl7: Model of M. pneumoniae 70S transertion-like (stable RNAP) -

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Basic information

Entry
Database: PDB / ID: 9sl7
TitleModel of M. pneumoniae 70S transertion-like (stable RNAP)
Components
  • (50S ribosomal protein ...) x 5
  • (DNA-directed RNA polymerase subunit ...) x 3
  • (Large ribosomal subunit protein ...) x 25
  • (Small ribosomal subunit protein ...) x 20
  • (Transcription termination/antitermination protein ...) x 2
  • 16S ribosomal RNA
  • 23S ribosomal RNA
  • 5S ribosomal RNA
  • DNA chain A
  • DNA chain B
  • Probable DNA-directed RNA polymerase subunit delta
  • SecDF
  • tRNA-Phe
KeywordsTRANSLATION / 70S / ribosome / RNAP / membrane
Function / homology
Function and homology information


DNA-templated transcription termination / DNA-templated transcription elongation / regulation of DNA-templated transcription elongation / transcription antitermination / ribonucleoside binding / DNA-directed RNA polymerase / DNA-directed RNA polymerase activity / transferase activity / large ribosomal subunit / ribosomal small subunit assembly ...DNA-templated transcription termination / DNA-templated transcription elongation / regulation of DNA-templated transcription elongation / transcription antitermination / ribonucleoside binding / DNA-directed RNA polymerase / DNA-directed RNA polymerase activity / transferase activity / large ribosomal subunit / ribosomal small subunit assembly / ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / 5S rRNA binding / ribosomal large subunit assembly / small ribosomal subunit / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / tRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / protein dimerization activity / response to antibiotic / rRNA binding / negative regulation of translation / ribonucleoprotein complex / structural constituent of ribosome / ribosome / translation / DNA-binding transcription factor activity / mRNA binding / regulation of DNA-templated transcription / magnesium ion binding / DNA-templated transcription / DNA binding / RNA binding / zinc ion binding / plasma membrane / cytosol / cytoplasm
Similarity search - Function
Transcription antitermination protein, NusG, mycoplasmataceae / DNA-directed RNA polymerase subunit delta / DNA-directed RNA polymerase subunit delta, N-terminal domain superfamily / ASXL, HARE-HTH domain / HB1, ASXL, restriction endonuclease HTH domain / HARE-type HTH domain profile. / : / : / : / NusA-like second KH domain ...Transcription antitermination protein, NusG, mycoplasmataceae / DNA-directed RNA polymerase subunit delta / DNA-directed RNA polymerase subunit delta, N-terminal domain superfamily / ASXL, HARE-HTH domain / HB1, ASXL, restriction endonuclease HTH domain / HARE-type HTH domain profile. / : / : / : / NusA-like second KH domain / Transcription termination factor NusA / Transcription factor NusA, N-terminal / KH domain, NusA-like / NusA, N-terminal domain superfamily / NusA N-terminal domain / NusA-like first KH domain / Transcription termination/antitermination protein NusA, bacterial / : / Transcription antitermination protein, NusG / NusG-like / Transcription termination factor nusG / Ribosomal protein L7/L12, oligomerisation / Ribosomal protein L7/L12, oligomerisation domain superfamily / Ribosomal protein L7/L12 dimerisation domain / Ribosomal protein L7/L12 / Ribosomal protein L7/L12, C-terminal / Ribosomal protein L7/L12 C-terminal domain / Ribosomal protein L7/L12, C-terminal/adaptor protein ClpS-like / NusG, N-terminal / In Spt5p, this domain may confer affinity for Spt4p. It possesses a RNP-like fold. / Type-1 KH domain profile. / NusG, N-terminal domain superfamily / Ribosomal protein uL10 C-terminal region / Ribosomal protein L10, eubacterial, conserved site / Ribosomal protein L10 signature. / Ribosomal protein L10 / : / : / Ribosomal protein S14, type Z / S1 domain profile. / Ribosomal protein L11, bacterial-type / DNA-directed RNA polymerase, subunit beta-prime, bacterial type / DNA-directed RNA polymerase, beta subunit, external 1 domain superfamily / DNA-directed RNA polymerase, beta subunit, external 1 domain / RNA polymerase beta subunit external 1 domain / RNA polymerase, alpha subunit, C-terminal / Bacterial RNA polymerase, alpha chain C terminal domain / DNA-directed RNA polymerase beta subunit, bacterial-type / DNA-directed RNA polymerase, alpha subunit / Ribosomal protein L31 type A / Ribosomal protein S1-like RNA-binding domain / S1 domain / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L11, conserved site / Ribosomal protein L11 signature. / Ribosomal protein L10-like domain superfamily / Ribosomal protein L10P / Ribosomal protein L10 / Ribosomal protein S21 / Ribosomal protein L9 signature. / Ribosomal protein L16 signature 1. / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L6, conserved site / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L6 signature 1. / Ribosomal protein L9, C-terminal domain superfamily / : / Ribosomal protein L11, N-terminal / Ribosomal protein L11, N-terminal domain / Ribosomal protein L16 signature 2. / Ribosomal protein L11/L12 / Ribosomal protein L11, C-terminal / Ribosomal protein L11, C-terminal domain superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily / Ribosomal protein L11/L12 / Ribosomal protein L16, conserved site / Ribosomal protein L11, RNA binding domain / Ribosomal protein L17 signature. / Ribosomal protein L36 signature. / : / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L28/L24 superfamily / Ribosomal protein L32p, bacterial type / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L9 / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L28 / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L18, bacterial-type / Ribosomal protein S6, conserved site
Similarity search - Domain/homology
: / DNA / DNA (> 10) / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS4 ...: / DNA / DNA (> 10) / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein bS21 / Transcription termination/antitermination protein NusG / Probable DNA-directed RNA polymerase subunit delta / Small ribosomal subunit protein bS16 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL28 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein uL13 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein bS20 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein bL12 / Large ribosomal subunit protein uL10 / DNA-directed RNA polymerase subunit beta' / Uncharacterized protein MG277 homolog / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL9 / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS12 / Large ribosomal subunit protein uL11 / Small ribosomal subunit protein uS2 / Large ribosomal subunit protein uL22 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL3 / Small ribosomal subunit protein uS10 / Transcription termination/antitermination protein NusA / Large ribosomal subunit protein bL34 / DNA-directed RNA polymerase subunit beta / Large ribosomal subunit protein bL33A / Large ribosomal subunit protein bL31 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL21 / DNA-directed RNA polymerase subunit alpha / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS13 / Large ribosomal subunit protein uL15 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS14 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL14 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein bL17
Similarity search - Component
Biological speciesMycoplasmoides pneumoniae M129 (bacteria)
MethodELECTRON MICROSCOPY / subtomogram averaging / cryo EM / Resolution: 26.7 Å
AuthorsDobbs, J.M. / Mahamid, J.
Funding support United States, Denmark, 2items
OrganizationGrant numberCountry
Chan Zuckerberg Initiative2021-234620 United States
Independent Research Fund Denmark - Medical Sciences0106-00010A Denmark
CitationJournal: Cell / Year: 2026
Title: Single-cell visual proteomics of a minimal bacterium reveals structural coordination of gene expression machineries.
Authors: Joseph M Dobbs / Rasmus K Jensen / Julia Mahamid /
Abstract: Translation is a central process in gene expression. Its regulation is complex, depends on factors that include cell state and the subcellular environment, and is subject to modulation via crosstalk ...Translation is a central process in gene expression. Its regulation is complex, depends on factors that include cell state and the subcellular environment, and is subject to modulation via crosstalk to processes such as transcription or translocation. Here, we used cryo-electron tomography of native and antibiotic-perturbed Mycoplasma pneumoniae cells to resolve 140 maps that recapitulate bacterial translation during the initiation, elongation, and recycling phases. We visualized multiple transcription-translation complexes, allowing us to propose a threading-based translation reinitiation mechanism and to provide structural evidence for a long-hypothesized supercomplex that coordinates transcription, translation, and membrane attachment. We resolved abundant membrane-associated large ribosomal subunits and suggest that dissociation from membranes depends on the conditional initiation of new translation, consistent with a potentially conserved mechanism in mammalian cells. This work visualizes the multilayered control of bacterial translation and demonstrates the power of in-cell structural biology to investigate regulatory circuits in gene expression.
History
DepositionSep 3, 2025Deposition site: PDBE / Processing site: PDBE
Revision 1.0Sep 23, 2026Provider: repository / Type: Initial release
Revision 1.1Oct 7, 2026Group: Database references / Category: citation / citation_author
Item: _citation.journal_abbrev / _citation.journal_id_CSD ..._citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _citation_author.identifier_ORCID

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
0: Large ribosomal subunit protein bL34
1: 50S ribosomal protein L35
2: 50S ribosomal protein L36
3: 23S ribosomal RNA
4: 5S ribosomal RNA
5: 16S ribosomal RNA
6: DNA-directed RNA polymerase subunit alpha
7: tRNA-Phe
8: SecDF
9: DNA-directed RNA polymerase subunit beta
A: Small ribosomal subunit protein uS2
AA: Probable DNA-directed RNA polymerase subunit delta
AB: DNA-directed RNA polymerase subunit alpha
AC: Transcription termination/antitermination protein NusA
AD: DNA chain A
AE: DNA chain B
AF: Transcription termination/antitermination protein NusG
B: Small ribosomal subunit protein uS3
C: Small ribosomal subunit protein uS4
D: Small ribosomal subunit protein uS5
E: Small ribosomal subunit protein bS6
F: Small ribosomal subunit protein uS7
G: Small ribosomal subunit protein uS8
H: Small ribosomal subunit protein uS9
I: Small ribosomal subunit protein uS10
J: Small ribosomal subunit protein uS11
K: Small ribosomal subunit protein uS12
L: Small ribosomal subunit protein uS13
M: Small ribosomal subunit protein uS14
N: Small ribosomal subunit protein uS15
O: Small ribosomal subunit protein bS16
P: Small ribosomal subunit protein uS17
Q: Small ribosomal subunit protein bS18
R: Small ribosomal subunit protein uS19
S: Small ribosomal subunit protein bS20
T: Small ribosomal subunit protein bS21
U: 50S ribosomal protein L10
V: Large ribosomal subunit protein bL12
W: Large ribosomal subunit protein bL12
X: Large ribosomal subunit protein bL12
Y: Large ribosomal subunit protein bL12
Z: DNA-directed RNA polymerase subunit beta'
a: Large ribosomal subunit protein uL2
b: Large ribosomal subunit protein uL3
c: Large ribosomal subunit protein uL4
d: Large ribosomal subunit protein uL5
e: Large ribosomal subunit protein uL6
f: Large ribosomal subunit protein bL9
h: Large ribosomal subunit protein uL11
i: Large ribosomal subunit protein uL13
j: 50S ribosomal protein L14
k: Large ribosomal subunit protein uL15
l: Large ribosomal subunit protein uL16
m: Large ribosomal subunit protein bL17
n: Large ribosomal subunit protein uL18
o: Large ribosomal subunit protein bL19
p: Large ribosomal subunit protein bL20
q: Large ribosomal subunit protein bL21
r: Large ribosomal subunit protein uL22
s: Large ribosomal subunit protein uL23
t: 50S ribosomal protein L24
u: Large ribosomal subunit protein bL27
v: Large ribosomal subunit protein bL28
w: Large ribosomal subunit protein uL29
x: Large ribosomal subunit protein bL31
y: Large ribosomal subunit protein bL32
z: Large ribosomal subunit protein bL33A


Theoretical massNumber of molelcules
Total (without water)2,729,23367
Polymers2,729,23367
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1

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Components

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Large ribosomal subunit protein ... , 25 types, 28 molecules 0VWXYabcdefhiklmnopqrsuvwxyz

#1: Protein/peptide Large ribosomal subunit protein bL34 / 50S ribosomal protein L34


Mass: 5459.579 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P78006
#37: Protein/peptide
Large ribosomal subunit protein bL12 / 50S ribosomal protein L7/L12


Mass: 3340.834 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75239
#39: Protein Large ribosomal subunit protein uL2 / 50S ribosomal protein L2


Mass: 31672.504 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75577
#40: Protein Large ribosomal subunit protein uL3 / 50S ribosomal protein L3


Mass: 25067.930 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75580
#41: Protein Large ribosomal subunit protein uL4 / 50S ribosomal protein L4


Mass: 23372.195 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75579
#42: Protein Large ribosomal subunit protein uL5 / 50S ribosomal protein L5


Mass: 19740.176 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: Q50306
#43: Protein Large ribosomal subunit protein uL6 / 50S ribosomal protein L6


Mass: 19789.082 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: Q50303
#44: Protein Large ribosomal subunit protein bL9 / 50S ribosomal protein L9


Mass: 16785.572 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75540
#45: Protein Large ribosomal subunit protein uL11 / 50S ribosomal protein L11


Mass: 13741.302 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75550
#46: Protein Large ribosomal subunit protein uL13 / 50S ribosomal protein L13


Mass: 16562.150 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75178
#48: Protein Large ribosomal subunit protein uL15 / 50S ribosomal protein L15


Mass: 16379.320 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: Q50300
#49: Protein Large ribosomal subunit protein uL16 / 50S ribosomal protein L16


Mass: 15389.190 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P41204
#50: Protein Large ribosomal subunit protein bL17 / 50S ribosomal protein L17


Mass: 13646.918 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: Q59547
#51: Protein Large ribosomal subunit protein uL18 / 50S ribosomal protein L18


Mass: 13073.256 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: Q50302
#52: Protein Large ribosomal subunit protein bL19 / 50S ribosomal protein L19


Mass: 13352.644 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75133
#53: Protein Large ribosomal subunit protein bL20 / 50S ribosomal protein L20


Mass: 13463.974 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P78023
#54: Protein Large ribosomal subunit protein bL21 / 50S ribosomal protein L21


Mass: 11549.737 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P78026
#55: Protein Large ribosomal subunit protein uL22 / 50S ribosomal protein L22


Mass: 15328.029 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75575
#56: Protein Large ribosomal subunit protein uL23 / 50S ribosomal protein L23


Mass: 10231.326 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75578
#58: Protein Large ribosomal subunit protein bL27 / 50S ribosomal protein L27


Mass: 9363.845 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75458
#59: Protein Large ribosomal subunit protein bL28 / 50S ribosomal protein L28


Mass: 7331.629 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75171
#60: Protein Large ribosomal subunit protein uL29 / 50S ribosomal protein L29


Mass: 12613.771 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: Q50310
#61: Protein/peptide Large ribosomal subunit protein bL31 / 50S ribosomal protein L31


Mass: 4936.662 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P78020
#62: Protein Large ribosomal subunit protein bL32 / 50S ribosomal protein L32


Mass: 6510.702 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75238
#63: Protein/peptide Large ribosomal subunit protein bL33A / 50S ribosomal protein L33 1


Mass: 5881.043 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P78015

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50S ribosomal protein ... , 5 types, 5 molecules 12Ujt

#2: Protein 50S ribosomal protein L35


Mass: 6793.135 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75447
#3: Protein/peptide 50S ribosomal protein L36


Mass: 4400.488 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P52864
#36: Protein 50S ribosomal protein L10


Mass: 17645.719 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75240
#47: Protein 50S ribosomal protein L14


Mass: 13478.906 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: Q50308
#57: Protein 50S ribosomal protein L24


Mass: 12461.865 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: Q50307

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RNA chain , 4 types, 4 molecules 3457

#4: RNA chain 23S ribosomal RNA


Mass: 931587.500 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria)
#5: RNA chain 5S ribosomal RNA


Mass: 33878.168 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria)
#6: RNA chain 16S ribosomal RNA


Mass: 482711.719 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria)
#8: RNA chain tRNA-Phe


Mass: 24470.521 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: GenBank: 3042778399

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DNA-directed RNA polymerase subunit ... , 3 types, 4 molecules 6AB9Z

#7: Protein DNA-directed RNA polymerase subunit alpha / RNAP subunit alpha / RNA polymerase subunit alpha / Transcriptase subunit alpha


Mass: 36709.496 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: Q50295, DNA-directed RNA polymerase
#10: Protein DNA-directed RNA polymerase subunit beta / RNAP subunit beta / RNA polymerase subunit beta / Transcriptase subunit beta


Mass: 155819.000 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P78013, DNA-directed RNA polymerase
#38: Protein DNA-directed RNA polymerase subunit beta' / RNAP subunit beta' / RNA polymerase subunit beta' / Transcriptase subunit beta'


Mass: 143333.938 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75271, DNA-directed RNA polymerase

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Protein , 2 types, 2 molecules 8AA

#9: Protein SecDF


Mass: 105125.898 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75387
#12: Protein Probable DNA-directed RNA polymerase subunit delta / RNAP delta factor


Mass: 10891.354 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75090

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Small ribosomal subunit protein ... , 20 types, 20 molecules ABCDEFGHIJKLMNOPQRST

#11: Protein Small ribosomal subunit protein uS2 / 30S ribosomal protein S2


Mass: 27362.705 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75560
#17: Protein Small ribosomal subunit protein uS3 / 30S ribosomal protein S3


Mass: 24162.953 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P41205
#18: Protein Small ribosomal subunit protein uS4 / 30S ribosomal protein S4


Mass: 23589.334 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P46775
#19: Protein Small ribosomal subunit protein uS5 / 30S ribosomal protein S5


Mass: 16715.732 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: Q50301
#20: Protein Small ribosomal subunit protein bS6 / 30S ribosomal protein S6


Mass: 19352.865 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75543
#21: Protein Small ribosomal subunit protein uS7 / 30S ribosomal protein S7


Mass: 17765.828 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75545
#22: Protein Small ribosomal subunit protein uS8 / 30S ribosomal protein S8


Mass: 15771.888 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: Q50304
#23: Protein Small ribosomal subunit protein uS9 / 30S ribosomal protein S9


Mass: 14603.050 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75179
#24: Protein Small ribosomal subunit protein uS10 / 30S ribosomal protein S10


Mass: 11524.757 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75581
#25: Protein Small ribosomal subunit protein uS11 / 30S ribosomal protein S11


Mass: 11877.700 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: Q50296
#26: Protein Small ribosomal subunit protein uS12 / 30S ribosomal protein S12


Mass: 15319.126 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75546
#27: Protein Small ribosomal subunit protein uS13 / 30S ribosomal protein S13


Mass: 13520.724 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: Q50297
#28: Protein Small ribosomal subunit protein uS14 / 30S ribosomal protein S14 type Z


Mass: 6770.168 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: Q50305
#29: Protein Small ribosomal subunit protein uS15 / 30S ribosomal protein S15


Mass: 9576.245 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75173
#30: Protein Small ribosomal subunit protein bS16 / 30S ribosomal protein S16


Mass: 9190.768 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75131
#31: Protein Small ribosomal subunit protein uS17 / 30S ribosomal protein S17


Mass: 9599.267 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: Q50309
#32: Protein Small ribosomal subunit protein bS18 / 30S ribosomal protein S18


Mass: 7631.100 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75541
#33: Protein Small ribosomal subunit protein uS19 / 30S ribosomal protein S19


Mass: 9682.159 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75576
#34: Protein Small ribosomal subunit protein bS20 / 30S ribosomal protein S20


Mass: 8977.407 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75237
#35: Protein Small ribosomal subunit protein bS21 / 30S ribosomal protein S21


Mass: 6724.104 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P57079

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Transcription termination/antitermination protein ... , 2 types, 2 molecules ACAF

#13: Protein Transcription termination/antitermination protein NusA


Mass: 41063.934 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75591
#16: Protein Transcription termination/antitermination protein NusG


Mass: 18151.113 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria) / References: UniProt: P75049

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DNA chain , 2 types, 2 molecules ADAE

#14: DNA chain DNA chain A


Mass: 9927.437 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria)
#15: DNA chain DNA chain B


Mass: 9749.313 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mycoplasmoides pneumoniae M129 (bacteria)

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Details

Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: CELL / 3D reconstruction method: subtomogram averaging

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Sample preparation

ComponentName: Mycoplasma cell / Type: CELL / Entity ID: all / Source: NATURAL
Source (natural)Organism: Mycoplasmoides pneumoniae M129 (bacteria)
Buffer solutionpH: 7
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE-PROPANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: TFS KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 5000 nm / Nominal defocus min: 2500 nm
Image recordingElectron dose: 3.2 e/Å2 / Avg electron dose per subtomogram: 120 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k)

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Processing

EM software
IDNameCategory
1Warpvolume selection
12M3D reconstruction
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
SymmetryPoint symmetry: C1 (asymmetric)
3D reconstructionResolution: 26.7 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 119 / Symmetry type: POINT
EM volume selectionNum. of tomograms: 254 / Num. of volumes extracted: 323168
Atomic model buildingProtocol: RIGID BODY FIT / Details: Rigid body fit in ChimeraX

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