EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody.
ジャーナル・号・ページ	Structure, Vol. 33, Issue 8, Page 1325-11336.e5, Year 2025
掲載日	2025年8月7日
著者	Qingbo Liu / Ruth J Parsons / Kevin Wiehe / Robert J Edwards / Kevin O Saunders / Peng Zhang / Huiyi Miao / Kedamawit Tilahun / Julia Jones / Yue Chen / Bhavna Hora / Wilton B Williams / David Easterhoff / Xiao Huang / Katarzyna Janowska / Katayoun Mansouri / Barton F Haynes / Priyamvada Acharya / Paolo Lusso /
PubMed 要旨	Although most broadly neutralizing antibodies (bNAbs) specific for the CD4-binding site (CD4-BS) of HIV-1 interact with a single gp120 protomer, a few mimic the quaternary binding mode of CD4, making ...Although most broadly neutralizing antibodies (bNAbs) specific for the CD4-binding site (CD4-BS) of HIV-1 interact with a single gp120 protomer, a few mimic the quaternary binding mode of CD4, making contact with a second protomer through elongated heavy chain framework 3 (FRH3) or complementarity-determining region 1 (CDRH1) loops. Here, we show that a CDRH3-dominated anti-CD4-BS bNAb, CH103, establishes quaternary interaction despite regular-length FRH3 and CDRH1. This quaternary interaction is critical for neutralization and is primarily mediated by two FRH3 acidic residues that were sequentially acquired and subjected to strong positive selection during CH103 maturation. Cryoelectron microscopy (cryo-EM) structures confirmed the role of the two FRH3 acidic residues in mediating quaternary contact and demonstrated that CH103 reaches the adjacent gp120 protomer by virtue of its unique angle of approach. Thus, the acquisition of quaternary interaction may constitute a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody.
リンク	Structure / PubMed:40412376 / PubMed Central
手法	EM (単粒子)
解像度	3.37 - 13.0 Å
構造データ	EMDB-44736: HIV Envelope trimer BG505 SOSIP.664 in complex with wild type CH103 antibody 手法: EM (単粒子) / 解像度: 13.0 Å EMDB-44738: HIV Envelope BG505 SOSIP.664 in complex with one Fab of CH103 E75K/D76N mutant 手法: EM (単粒子) / 解像度: 10.0 Å EMDB-44739: HIV Envelope trimer CH505 SOSIP.664 in complex with wild type CH103 antibody 手法: EM (単粒子) / 解像度: 12.0 Å EMDB-44740: HIV Envelope trimer CH505 SOSIP.664 in complex with three CH103 E75K/D76N mutant antibody Fabs 手法: EM (単粒子) / 解像度: 10.0 Å 46604 9d7g EMDB-46604, PDB-9d7g: BG505 DS-SOSIP.664 apo structure from the CH103 KN cryo-EM dataset 手法: EM (単粒子) / 解像度: 3.58 Å 46605 9d7h EMDB-46605, PDB-9d7h: Cryo-EM structure of BG505 DS-SOSIP.664 with 1 CH103 KN Fab bound 手法: EM (単粒子) / 解像度: 3.59 Å 46606 9d7i EMDB-46606, PDB-9d7i: Cryo-EM structure of BG505 DS-SOSIP.664 with 2 CH103 KN Fabs bound 手法: EM (単粒子) / 解像度: 3.68 Å 46613 9d7o EMDB-46613, PDB-9d7o: Cryo-EM structure of BG505 DS-SOSIP.664 with 1 CH103 Fab bound 手法: EM (単粒子) / 解像度: 3.56 Å 46614 9d7p EMDB-46614, PDB-9d7p: Cryo-EM structure of BG505 DS-SOSIP.664 with 2 CH103 Fabs bound 手法: EM (単粒子) / 解像度: 3.37 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン
由来	Human immunodeficiency virus 2 (ヒト免疫不全ウイルス) homo sapiens (ヒト) human immunodeficiency virus 1 (ヒト免疫不全ウイルス)
キーワード	VIRAL PROTEIN / Trimer / Env / BG505 / CH103 / VIRAL PROTEIN/IMMUNE SYSTEM / VIRAL PROTEIN-IMMUNE SYSTEM complex