Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Fascin structural plasticity mediates flexible actin bundle construction.
Journal, issue, pages	Nat Struct Mol Biol, Vol. 32, Issue 5, Page 940-952, Year 2025
Publish date	Jan 20, 2025
Authors	Rui Gong / Matthew J Reynolds / Keith R Carney / Keith Hamilton / Tamara C Bidone / Gregory M Alushin /
PubMed Abstract	Fascin cross-links actin filaments (F-actin) into bundles that support tubular membrane protrusions including filopodia and stereocilia. Fascin dysregulation drives aberrant cell migration during ...Fascin cross-links actin filaments (F-actin) into bundles that support tubular membrane protrusions including filopodia and stereocilia. Fascin dysregulation drives aberrant cell migration during metastasis, and fascin inhibitors are under development as cancer therapeutics. Here, we use cryo-EM, cryo-electron tomography coupled with custom denoising and computational modeling to probe human fascin-1's F-actin cross-linking mechanisms across spatial scales. Our fascin cross-bridge structure reveals an asymmetric F-actin binding conformation that is allosterically blocked by the inhibitor G2. Reconstructions of seven-filament hexagonal bundle elements, variability analysis and simulations show how structural plasticity enables fascin to bridge varied interfilament orientations, accommodating mismatches between F-actin's helical symmetry and bundle hexagonal packing. Tomography of many-filament bundles and modeling uncover geometric rules underlying emergent fascin binding patterns, as well as the accumulation of unfavorable cross-links that limit bundle size. Collectively, this work shows how fascin harnesses fine-tuned nanoscale structural dynamics to build and regulate micron-scale F-actin bundles.
External links	Nat Struct Mol Biol / PubMed:39833469 / PubMed Central
Methods	EM (single particle) / EM (subtomogram averaging)
Resolution	3.0 - 12.0 Å
Structure data	43364 8vo5 EMDB-43364, PDB-8vo5: Cryo-EM structure of fascin bound to F-actin (actin binding site 1) Method: EM (single particle) / Resolution: 3.0 Å 43365 8vo6 EMDB-43365, PDB-8vo6: Cryo-EM structure of fascin bound to F-actin (actin binding site 2) Method: EM (single particle) / Resolution: 3.1 Å 43366 8vo7 EMDB-43366, PDB-8vo7: Cryo-EM structure of fascin crosslinked F-actin Method: EM (single particle) / Resolution: 3.1 Å 43367 8vo8 EMDB-43367, PDB-8vo8: Cryo-EM structure of fascin crosslinked F-actin (Eigen_left) Method: EM (single particle) / Resolution: 3.9 Å 43368 8vo9 EMDB-43368, PDB-8vo9: Cryo-EM structure of fascin crosslinked F-actin (Eigen_middle) Method: EM (single particle) / Resolution: 3.4 Å 43369 8voa EMDB-43369, PDB-8voa: Cryo-EM structure of fascin crosslinked F-actin (Eigen_right) Method: EM (single particle) / Resolution: 4.0 Å EMDB-43370: Fascin crosslinked F-actin hexagonal bundle element (460 Angstrom box) Method: EM (single particle) / Resolution: 8.7 Å EMDB-43371: Fascin crosslinked F-actin hexagonal bundle element (740 Angstrom box) Method: EM (single particle) / Resolution: 12.0 Å EMDB-43372: Subtomogram averaging structure of fascin bound to F-actin Method: EM (subtomogram averaging) / Resolution: 6.7 Å EMDB-43764: cryo-EM structure of fascin crosslinked F-actin (containing both actin binding sites) Method: EM (single particle) / Resolution: 3.39 Å
Chemicals	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying moleculeYM ChemComp-MG*: Unknown entry
Source	homo sapiens (human) gallus gallus (chicken)
Keywords	STRUCTURAL PROTEIN / Cytoskeleton / F-actin crosslinker / F-actin bundle