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TitleMolecular basis of Wnt biogenesis, secretion, and Wnt7-specific signaling.
Journal, issue, pagesCell, Vol. 186, Issue 23, Page 5028-5040.e14, Year 2023
Publish dateNov 9, 2023
AuthorsXiaofeng Qi / Qinli Hu / Nadia Elghobashi-Meinhardt / Tao Long / Hongwen Chen / Xiaochun Li /
PubMed AbstractWnt proteins are enzymatically lipidated by Porcupine (PORCN) in the ER and bind to Wntless (WLS) for intracellular transport and secretion. Mechanisms governing the transfer of these low-solubility ...Wnt proteins are enzymatically lipidated by Porcupine (PORCN) in the ER and bind to Wntless (WLS) for intracellular transport and secretion. Mechanisms governing the transfer of these low-solubility Wnts from the ER to the extracellular space remain unclear. Through structural and functional analyses of Wnt7a, a crucial Wnt involved in central nervous system angiogenesis and blood-brain barrier maintenance, we have elucidated the principles of Wnt biogenesis and Wnt7-specific signaling. The Wnt7a-WLS complex binds to calreticulin (CALR), revealing that CALR functions as a chaperone to facilitate Wnt transfer from PORCN to WLS during Wnt biogenesis. Our structures, functional analyses, and molecular dynamics simulations demonstrate that a phospholipid in the core of Wnt-bound WLS regulates the association and dissociation between Wnt and WLS, suggesting a lipid-mediated Wnt secretion mechanism. Finally, the structure of Wnt7a bound to RECK, a cell-surface Wnt7 co-receptor, reveals how RECK engages the N-terminal domain of Wnt7a to activate Wnt7-specific signaling.
External linksCell / PubMed:37852257 / PubMed Central
MethodsEM (single particle)
Resolution3.1 - 3.84 Å
Structure data

EMDB-41764, PDB-8tzo:
Structure of human Wnt7a bound to WLS and CALR
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-41765, PDB-8tzp:
Structure of human Wnt7a bound to WLS and RECK
Method: EM (single particle) / Resolution: 3.23 Å

EMDB-41768, PDB-8tzs:
Structure of human WLS
Method: EM (single particle) / Resolution: 3.84 Å

Chemicals

ChemComp-PAM:
PALMITOLEIC ACID

ChemComp-POV:
(2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate / phospholipid*YM

ChemComp-CA:
Unknown entry

Source
  • homo sapiens (human)
KeywordsSIGNALING PROTEIN / MEMBRANE PROTEIN

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