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TitleStructure-based validation of recombinant light-harvesting complex II.
Journal, issue, pagesPNAS Nexus, Vol. 3, Issue 9, Page pgae405, Year 2024
Publish dateSep 25, 2024
AuthorsSoichiro Seki / Tomoko Miyata / Naoko Norioka / Hideaki Tanaka / Genji Kurisu / Keiichi Namba / Ritsuko Fujii /
PubMed AbstractLight-harvesting complex II (LHCII) captures sunlight and dissipates excess energy to drive photosynthesis. To elucidate this mechanism, the individual optical properties of pigments in the LHCII ...Light-harvesting complex II (LHCII) captures sunlight and dissipates excess energy to drive photosynthesis. To elucidate this mechanism, the individual optical properties of pigments in the LHCII protein must be identified. In vitro reconstitution with apoproteins synthesized by and pigment-lipid mixtures from natural sources is an effective approach; however, the local environment surrounding each pigment within reconstituted LHCII (rLHCII) has only been indirectly estimated using spectroscopic and biochemical methods. Here, we used cryo-electron microscopy to determine the 3D structure of the rLHCII trimer and found that rLHCII exhibited a structure that was virtually identical to that of native LHCII, with a few exceptions: some C-terminal amino acids were not visible, likely due to aggregation of the His-tags; a carotenoid at the V1 site was not visible; and at site 614 showed mixed occupancy by both chlorophyll and molecules. Our observations confirmed the applicability of the in vitro reconstitution technique.
External linksPNAS Nexus / PubMed:39346626 / PubMed Central
MethodsEM (single particle)
Resolution2.17 - 2.57 Å
Structure data

38825

8y15

EMDB-38825, PDB-8y15:
Cryo-EM structure of Light-harvesting complex from Spinacia oleracea
Method: EM (single particle) / Resolution: 2.17 Å

39192

8yee

EMDB-39192, PDB-8yee:
Cryo-EM structure of in vitro reconstituted Light-harvesting complex II trimer
Method: EM (single particle) / Resolution: 2.37 Å

EMDB-39304: Cryo-EM structure of light-harvesting complex II with C1 symmetry
Method: EM (single particle) / Resolution: 2.32 Å

EMDB-39305: Cryo-EM structure of in vitro reconstituted LHCII with C1 symmetry
Method: EM (single particle) / Resolution: 2.57 Å

Chemicals

ChemComp-CHL:
CHLOROPHYLL B

ChemComp-CLA:
CHLOROPHYLL A


PDB Unreleased entry

PDB-1lxp: SOLUTION STRUCTURE OF HCV IRES DOMAIN II

ChemComp-NEX:
(1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM

ChemComp-XAT:
(3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL

ChemComp-HOH:
WATER

Source
  • spinacia oleracea (spinach)
KeywordsPHOTOSYNTHESIS / Complex / light-harvesting / membrane protein

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