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TitleStructural insights into the targeting specificity of ubiquitin ligase for S. cerevisiae isocitrate lyase but not C. albicans isocitrate lyase.
Journal, issue, pagesJ Struct Biol, Vol. 213, Issue 3, Page 107748, Year 2021
Publish dateMay 24, 2021
AuthorsKeito Hiragi / Kazuya Nishio / Shu Moriyama / Tasuku Hamaguchi / Akira Mizoguchi / Koji Yonekura / Kazutoshi Tani / Tsunehiro Mizushima /
PubMed AbstractIn Saccharomyces cerevisiae, the glyoxylate cycle is controlled through the posttranslational regulation of its component enzymes, such as isocitrate lyase (ICL), which catalyzes the first unique ...In Saccharomyces cerevisiae, the glyoxylate cycle is controlled through the posttranslational regulation of its component enzymes, such as isocitrate lyase (ICL), which catalyzes the first unique step of the cycle. The ICL of S.cerevisiae (ScIcl1) is tagged for proteasomal degradation through ubiquitination by a multisubunit ubiquitin ligase (the glucose-induced degradation-deficient (GID) complex), whereas that of the pathogenic yeast Candida albicans (CaIcl1) escapes this process. However, the reason for the ubiquitin targeting specificity of the GID complex for ScIcl1 and not for CaIcl1 is unclear. To gain some insight into this, in this study, the crystal structures of apo ScIcl1 and CaIcl1 in complex with formate and the cryogenic electron microscopy structure of apo CaIcl1 were determined at a resolution of 2.3, 2.7, and 2.6 Å, respectively. A comparison of the various structures suggests that the orientation of N-terminal helix α1 in S.cerevisiae is likely key to repositioning of ubiquitination sites and contributes to the distinction found in C. albicans ubiquitin evasion mechanism. This finding gives us a better understanding of the molecular mechanism of ubiquitin-dependent ScIcl1 degradation and could serve as a theoretical basis for the research and development of anti-C. albicans drugs based on the concept of CaIcl1 ubiquitination.
External linksJ Struct Biol / PubMed:34033899
MethodsEM (single particle) / X-ray diffraction
Resolution2.3 - 2.69 Å
Structure data

31053

7ebf

EMDB-31053, PDB-7ebf:
Cryo-EM structure of Isocitrate lyase-1 from Candida albicans
Method: EM (single particle) / Resolution: 2.63 Å

PDB-7ebc:
Crystal structure of Isocitrate lyase-1 from Saccaromyces cervisiae
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-7ebe:
Crystal structure of Isocitrate lyase-1 from Candida albicans
Method: X-RAY DIFFRACTION / Resolution: 2.69 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM / Polyethylene glycol

ChemComp-HOH:
WATER / Water

ChemComp-FMT:
FORMIC ACID / Formic acid

Source
  • candida albicans (yeast)
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsLYASE / Isocitrate lyase / glyoxylate cycle / ubiquitination

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