Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Precisely patterned nanofibres made from extendable protein multiplexes.
Journal, issue, pages	Nat Chem, Vol. 15, Issue 12, Page 1664-1671, Year 2023
Publish date	Sep 4, 2023
Authors	Neville P Bethel / Andrew J Borst / Fabio Parmeggiani / Matthew J Bick / T J Brunette / Hannah Nguyen / Alex Kang / Asim K Bera / Lauren Carter / Marcos C Miranda / Ryan D Kibler / Mila Lamb / Xinting Li / Banumathi Sankaran / David Baker /
PubMed Abstract	Molecular systems with coincident cyclic and superhelical symmetry axes have considerable advantages for materials design as they can be readily lengthened or shortened by changing the length of the ...Molecular systems with coincident cyclic and superhelical symmetry axes have considerable advantages for materials design as they can be readily lengthened or shortened by changing the length of the constituent monomers. Among proteins, alpha-helical coiled coils have such symmetric, extendable architectures, but are limited by the relatively fixed geometry and flexibility of the helical protomers. Here we describe a systematic approach to generating modular and rigid repeat protein oligomers with coincident C to C and superhelical symmetry axes that can be readily extended by repeat propagation. From these building blocks, we demonstrate that a wide range of unbounded fibres can be systematically designed by introducing hydrophilic surface patches that force staggering of the monomers; the geometry of such fibres can be precisely tuned by varying the number of repeat units in the monomer and the placement of the hydrophilic patches.
External links	Nat Chem / PubMed:37667012 / PubMed Central
Methods	EM (single particle) / EM (helical sym.) / X-ray diffraction
Resolution	1.59 - 7.62 Å
Structure data	EMDB-29680: C6HR1_8r: Extendable repeat protein heptamer Method: EM (single particle) / Resolution: 4.18 Å EMDB-29847: C3HR3_8r: Extendable repeat protein trimer Method: EM (single particle) / Resolution: 5.58 Å 29849 8gaa EMDB-29849, PDB-8gaa: C6HR1_4r: Extendable repeat protein hexamer Method: EM (single particle) / Resolution: 4.24 Å EMDB-29851: C4HR1_8r_shift5: Extendable repeat protein fiber Method: EM (helical sym.) / Resolution: 7.62 Å 29856 8g8i EMDB-29856, PDB-8g8i: C3HR3_9r_shift4: Extendable repeat protein fiber Method: EM (helical sym.) / Resolution: 3.92 Å 29894 8ga9 EMDB-29894, PDB-8ga9: C4HR1_4r: Extendable repeat protein tetramer Method: EM (single particle) / Resolution: 3.71 Å 29904 8gaq EMDB-29904, PDB-8gaq: C5HR2_4r: Extendable repeat protein pentamer Method: EM (single particle) / Resolution: 3.73 Å PDB-8eov: Precisely patterned nanofibers made from extendable protein multiplexes Method: X-RAY DIFFRACTION / Resolution: 1.59 Å PDB-8eox: Precisely patterned nanofibers made from extendable protein multiplexes Method: X-RAY DIFFRACTION / Resolution: 3.3 Å PDB-8eoz: Precisely patterned nanofibers made from extendable protein multiplexes Method: X-RAY DIFFRACTION / Resolution: 3.0 Å PDB-8erw: Precisely patterned nanofibers made from extendable protein multiplexes Method: X-RAY DIFFRACTION / Resolution: 2.88 Å
Chemicals	ChemComp-PO4: PHOSPHATE ION ChemComp-HOH: WATER
Source	unidentified (others) synthetic construct (others)
Keywords	DE NOVO PROTEIN / de novo design / nanofibers / Oligomer / repeat protein