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Structure paper

TitleMolecular mechanisms of assembly and TRIP13-mediated remodeling of the human Shieldin complex.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 118, Issue 8, Year 2021
Publish dateFeb 23, 2021
AuthorsWei Xie / Shengliu Wang / Juncheng Wang / M Jason de la Cruz / Guotai Xu / Maurizio Scaltriti / Dinshaw J Patel /
PubMed AbstractThe Shieldin complex, composed of REV7, SHLD1, SHLD2, and SHLD3, protects DNA double-strand breaks (DSBs) to promote nonhomologous end joining. The AAA ATPase TRIP13 remodels Shieldin to regulate DNA ...The Shieldin complex, composed of REV7, SHLD1, SHLD2, and SHLD3, protects DNA double-strand breaks (DSBs) to promote nonhomologous end joining. The AAA ATPase TRIP13 remodels Shieldin to regulate DNA repair pathway choice. Here we report crystal structures of human SHLD3-REV7 binary and fused SHLD2-SHLD3-REV7 ternary complexes, revealing that assembly of Shieldin requires fused SHLD2-SHLD3 induced conformational heterodimerization of open (O-REV7) and closed (C-REV7) forms of REV7. We also report the cryogenic electron microscopy (cryo-EM) structures of the ATPγS-bound fused SHLD2-SHLD3-REV7-TRIP13 complexes, uncovering the principles underlying the TRIP13-mediated disassembly mechanism of the Shieldin complex. We demonstrate that the N terminus of REV7 inserts into the central channel of TRIP13, setting the stage for pulling the unfolded N-terminal peptide of C-REV7 through the central TRIP13 hexameric channel. The primary interface involves contacts between the safety-belt segment of C-REV7 and a conserved and negatively charged loop of TRIP13. This process is mediated by ATP hydrolysis-triggered rotatory motions of the TRIP13 ATPase, thereby resulting in the disassembly of the Shieldin complex.
External linksProc Natl Acad Sci U S A / PubMed:33597306 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution2.7 - 3.8 Å
Structure data

23244

7l9p

EMDB-23244, PDB-7l9p:
Structure of human SHLD2-SHLD3-REV7-TRIP13(E253Q) complex
Method: EM (single particle) / Resolution: 3.6 Å

PDB-6ww9:
Crystal structure of human REV7(R124A)-SHLD3(35-58) complex
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-6wwa:
Crystal structure of human SHLD2-SHLD3-REV7 complex
Method: X-RAY DIFFRACTION / Resolution: 3.8 Å

Chemicals

ChemComp-AGS:
PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-gamma-S, energy-carrying molecule analogue*YM

Source
  • homo sapiens (human)
KeywordsNUCLEAR PROTEIN / REV7 / SHLD3 / SHLD2 / TRIP13

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