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TitleMechanism of polyadenylation-independent RNA polymerase II termination.
Journal, issue, pagesNat Struct Mol Biol, Vol. 32, Issue 2, Page 339-345, Year 2025
Publish dateOct 18, 2024
AuthorsSrinivasan Rengachari / Thomas Hainthaler / Christiane Oberthuer / Michael Lidschreiber / Patrick Cramer /
PubMed AbstractThe mechanisms underlying the initiation and elongation of RNA polymerase II (Pol II) transcription are well-studied, whereas termination remains poorly understood. Here we analyze the mechanism of ...The mechanisms underlying the initiation and elongation of RNA polymerase II (Pol II) transcription are well-studied, whereas termination remains poorly understood. Here we analyze the mechanism of polyadenylation-independent Pol II termination mediated by the yeast Sen1 helicase. Cryo-electron microscopy structures of two pretermination intermediates show that Sen1 binds to Pol II and uses its adenosine triphosphatase activity to pull on exiting RNA in the 5' direction. This is predicted to push Pol II forward, induce an unstable hypertranslocated state and destabilize the transcription bubble, thereby facilitating termination. This mechanism of transcription termination may be widely used because it is conceptually conserved in the bacterial transcription system.
External linksNat Struct Mol Biol / PubMed:39424994 / PubMed Central
MethodsEM (single particle)
Resolution2.8 - 4.4 Å
Structure data

19019

8ram

EMDB-19019, PDB-8ram:
Structure of Sen1 bound RNA Polymerase II pre-termination complex
Method: EM (single particle) / Resolution: 2.8 Å

19020

8ran

EMDB-19020, PDB-8ran:
Structure of Sen1-RNA complex
Method: EM (single particle) / Resolution: 3.25 Å

19021

8rao

EMDB-19021, PDB-8rao:
Structure of Sen1-ADP.BeF3-RNA complex
Method: EM (single particle) / Resolution: 4.4 Å

19022

8rap

EMDB-19022, PDB-8rap:
Structure of Sen1-ADP.BeF3 bound RNA Polymerase II pre-termination complex
Method: EM (single particle) / Resolution: 4.3 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-BEF:
BERYLLIUM TRIFLUORIDE ION

Source
  • saccharomyces cerevisiae (brewer's yeast)
  • human immunodeficiency virus 2
KeywordsGENE REGULATION / RNA Polymerase II / Pol II / termination / Sen1

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