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-Structure paper
Title | Visualizing chaperonin function in situ by cryo-electron tomography |
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Journal, issue, pages | Nature, Year 2024 |
Publish date | May 23, 2023 (structure data deposition date) |
![]() | Wagner J / Caravajal AI / Beck F / Bracher A / Wan W / Bohn S / Koerner R / Baumeister W / Fernandez-Busnadiego R / Hartl FU |
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Methods | EM (single particle) / EM (subtomogram averaging) |
Resolution | 2.5 - 15.2 Å |
Structure data | EMDB-17418, PDB-8p4m: EMDB-17420, PDB-8p4n: EMDB-17421, PDB-8p4o: ![]() EMDB-17422: Density for MetK encapsulated in the GroEL7-GroES7 cage ![]() EMDB-17423: Symmetry-averaged GroEL7-GroES7 chamber with encapsulated disordered substrate MetK obtained by in vitro cryo electron tomography ![]() EMDB-17424: Symmetry-averaged GroEL7-GroES7 chamber with encapsulated ordered substrate MetK obtained by in vitro cryo electron tomography EMDB-17425, PDB-8p4p: EMDB-17426, PDB-8p4r: ![]() EMDB-17534: Cryo-EM structure of a D7-symmetrical GroEL14-GroES14 complex in presence of ADP-BeFx ![]() EMDB-17535: Cryo-EM structure of a C7-symmetrical GroEL14-GroES7 complex in presence of ADP-BeFx ![]() EMDB-17559: In situ structure average of GroEL7-GroES7 chamber with no or disordered substrate in Escherichia coli cytosol obtained by cryo electron tomography ![]() EMDB-17560: In situ structure average of GroEL7-GroES7 chamber with encapsulated, ordered substrate in Escherichia coli cytosol obtained by cryo electron tomography ![]() EMDB-17561: Cryo-ET subtomogram of 70S ribosomes in Escherichia coli cells at 37 and 46 degrees centigrade and in Escherichia coli cells overexpressing GroELS and MetK ![]() EMDB-17562: Cryo-ET subtomogram of 70S ribosomes in Escherichia coli cells overexpressing GroEL ![]() EMDB-17563: CryoEM structure of a GroEL14-GroES7 cage with encapsulated ordered substrate MetK in the presence of ADP-BeFx ![]() EMDB-17564: CryoEM structure of a GroEL14-GroES7 cage with encapsulated disordered substrate MetK in the presence of ADP-BeFx ![]() EMDB-17565: CryoEM structure of a GroEL14-GroES14 cage with two encapsulated disordered MetK substrates in the presence of ADP-BeFx ![]() EMDB-17566: CryoEM structure of a (GroEL)14-(GroES)14 complex with encapsulated ordered MetK substrate in one chamber and no or disordered MetK substrate in the other chamber in the presence of ADP-BeFx ![]() EMDB-17567: Conformer 1 of the (GroEL)14(GroES)14 complex with two encapsulated, ordered and near-native MetK substrate molecules in the presence of ADP-BeFx ![]() EMDB-17568: Conformer 2 of the (GroEL)14(GroES)14 complex with two encapsulated, near-native and ordered MetK substrates in the presence of ADP-BeFx ![]() EMDB-17569: Conformer 3 of the (GroEL)14(GroES)14 complex with two encapsulated, near-native and ordered MetK substrates in the presence of ADP-BeFx ![]() EMDB-17570: Conformer 4 of the (GroEL)14(GroES)14 complex with two encapsulated, near-native and ordered MetK substrates in the presence of ADP-BeFx ![]() EMDB-17571: Conformer 5 of the (GroEL)14(GroES)14 complex with two encapsulated, near-native and ordered MetK substrates in the presence of ADP-BeFx ![]() EMDB-17572: Conformer 6 of the (GroEL)14(GroES)14 complex with two encapsulated, near-native and ordered MetK substrates in the presence of ADP-BeFx ![]() EMDB-17573: Conformer 7 of the (GroEL)14(GroES)14 complex with two encapsulated, near-native and ordered MetK substrates in the presence of ADP-BeFx EMDB-18735, PDB-8qxs: EMDB-18736, PDB-8qxt: EMDB-18737, PDB-8qxu: EMDB-18738, PDB-8qxv: |
Chemicals | ![]() ChemComp-ADP: ![]() ChemComp-MG: ![]() ChemComp-BEF: ![]() ChemComp-K: ![]() ChemComp-HOH: ![]() ChemComp-ATP: |
Source |
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![]() | CHAPERONE / Chaperonin / Folding cage / proteostasis / heat shock / ATPase |