Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	mRNA recognition and packaging by the human transcription-export complex.
Journal, issue, pages	Nature, Vol. 616, Issue 7958, Page 828-835, Year 2023
Publish date	Apr 5, 2023
Authors	Belén Pacheco-Fiallos / Matthias K Vorländer / Daria Riabov-Bassat / Laura Fin / Francis J O'Reilly / Farja I Ayala / Ulla Schellhaas / Juri Rappsilber / Clemens Plaschka /
PubMed Abstract	Newly made mRNAs are processed and packaged into mature ribonucleoprotein complexes (mRNPs) and are recognized by the essential transcription-export complex (TREX) for nuclear export. However, the ...Newly made mRNAs are processed and packaged into mature ribonucleoprotein complexes (mRNPs) and are recognized by the essential transcription-export complex (TREX) for nuclear export. However, the mechanisms of mRNP recognition and three-dimensional mRNP organization are poorly understood. Here we report cryo-electron microscopy and tomography structures of reconstituted and endogenous human mRNPs bound to the 2-MDa TREX complex. We show that mRNPs are recognized through multivalent interactions between the TREX subunit ALYREF and mRNP-bound exon junction complexes. Exon junction complexes can multimerize through ALYREF, which suggests a mechanism for mRNP organization. Endogenous mRNPs form compact globules that are coated by multiple TREX complexes. These results reveal how TREX may simultaneously recognize, compact and protect mRNAs to promote their packaging for nuclear export. The organization of mRNP globules provides a framework to understand how mRNP architecture facilitates mRNA biogenesis and export.
External links	Nature / PubMed:37020021 / PubMed Central
Methods	EM (single particle) / EM (tomography)
Resolution	2.4 - 7.5 Å
Structure data	14803 7znj EMDB-14803, PDB-7znj: Structure of an ALYREF-exon junction complex hexamer Method: EM (single particle) / Resolution: 2.4 Å 14804 7znk EMDB-14804: Structure of an endogenous human TREX complex bound to mRNA, composite map PDB-7znk: Structure of an endogenous human TREX complex bound to mRNA Method: EM (single particle) / Resolution: 3.9 Å EMDB-14805: Structure of an endogenous human TREX complex bound to mRNA, map A Method: EM (single particle) / Resolution: 3.9 Å EMDB-14806: Structure of an endogenous human TREX complex bound to mRNA, map B Method: EM (single particle) / Resolution: 5.5 Å EMDB-14807: Structure of an endogenous human TREX complex bound to mRNA, map C Method: EM (single particle) / Resolution: 7.5 Å 14808 7znl EMDB-14808: Structure of the human TREX core THO-UAP56 complex (map D) PDB-7znl: Structure of the human TREX core THO-UAP56 complex Method: EM (single particle) / Resolution: 3.45 Å EMDB-14809: Structure of the human TREX core THO-UAP56 complex (map E) Method: EM (single particle) / Resolution: 3.9 Å EMDB-16633: Structure of an ALYREF-exon junction complex hexamer, obtained from TREX-EJC-RNA sample Method: EM (single particle) / Resolution: 3.0 Å EMDB-16753: Tomogram of purified human TREX-mRNPs Method: EM (tomography) EMDB-17215: Composite map of the human TREX core THO-UAP56 complex Method: EM (single particle) / Resolution: 3.3 Å
Chemicals	ChemComp-MG: Unknown entry ChemComp-ANP: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM
Source	homo sapiens (human)
Keywords	GENE REGULATION / transcription-exort complex / TREX / splicing / exon junction complex / EJC / RNA export / RNA binding proteins / transcription and export complex / RNA packaging / RNA binding protein / gene expression