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- EMDB-14804: Structure of an endogenous human TREX complex bound to mRNA, comp... -
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Open data
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Basic information
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Title | Structure of an endogenous human TREX complex bound to mRNA, composite map | |||||||||
![]() | TREX-mRNA composite map generated from sharpened focused maps A (EMD 14805),B (EMD-14806) and C (EMD-14807 ) | |||||||||
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Function / homology | ![]() THO complex / THO complex part of transcription export complex / primitive hemopoiesis / transcription export complex / C5-methylcytidine-containing RNA reader activity / regulation of mRNA export from nucleus / U6 snRNP / RNA secondary structure unwinding / stem cell division / Transport of the SLBP independent Mature mRNA ...THO complex / THO complex part of transcription export complex / primitive hemopoiesis / transcription export complex / C5-methylcytidine-containing RNA reader activity / regulation of mRNA export from nucleus / U6 snRNP / RNA secondary structure unwinding / stem cell division / Transport of the SLBP independent Mature mRNA / Transport of the SLBP Dependant Mature mRNA / mRNA 3'-end processing / ATP-dependent activity, acting on RNA / ATP-dependent protein binding / Transport of Mature mRNA Derived from an Intronless Transcript / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Pacheco-Fiallos FB / Vorlaender MK / Plaschka C | |||||||||
Funding support | European Union, 2 items
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![]() | ![]() Title: mRNA recognition and packaging by the human transcription-export complex. Authors: Belén Pacheco-Fiallos / Matthias K Vorländer / Daria Riabov-Bassat / Laura Fin / Francis J O'Reilly / Farja I Ayala / Ulla Schellhaas / Juri Rappsilber / Clemens Plaschka / ![]() ![]() ![]() Abstract: Newly made mRNAs are processed and packaged into mature ribonucleoprotein complexes (mRNPs) and are recognized by the essential transcription-export complex (TREX) for nuclear export. However, the ...Newly made mRNAs are processed and packaged into mature ribonucleoprotein complexes (mRNPs) and are recognized by the essential transcription-export complex (TREX) for nuclear export. However, the mechanisms of mRNP recognition and three-dimensional mRNP organization are poorly understood. Here we report cryo-electron microscopy and tomography structures of reconstituted and endogenous human mRNPs bound to the 2-MDa TREX complex. We show that mRNPs are recognized through multivalent interactions between the TREX subunit ALYREF and mRNP-bound exon junction complexes. Exon junction complexes can multimerize through ALYREF, which suggests a mechanism for mRNP organization. Endogenous mRNPs form compact globules that are coated by multiple TREX complexes. These results reveal how TREX may simultaneously recognize, compact and protect mRNAs to promote their packaging for nuclear export. The organization of mRNP globules provides a framework to understand how mRNP architecture facilitates mRNA biogenesis and export. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 8.2 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 23.7 KB 23.7 KB | Display Display | ![]() |
Images | ![]() | 118.2 KB | ||
Others | ![]() | 8.3 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7znkMC ![]() 7znjC ![]() 7znlC C: citing same article ( M: atomic model generated by this map |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Annotation | TREX-mRNA composite map generated from sharpened focused maps A (EMD 14805),B (EMD-14806) and C (EMD-14807 ) | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.41 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: TREX-mRNA composite map generated from unsharpened focused maps...
File | emd_14804_additional_1.map | ||||||||||||
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Annotation | TREX-mRNA composite map generated from unsharpened focused maps A (EMD 14805),B (EMD-14806) and C (EMD-14807 | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
+Entire : endogenous human TREX-mRNA complex
+Supramolecule #1: endogenous human TREX-mRNA complex
+Macromolecule #1: RNA
+Macromolecule #2: THO complex subunit 1
+Macromolecule #3: THO complex subunit 2
+Macromolecule #4: THO complex subunit 3
+Macromolecule #5: THO complex subunit 5 homolog
+Macromolecule #6: THO complex subunit 6 homolog
+Macromolecule #7: THO complex subunit 7 homolog
+Macromolecule #8: Spliceosome RNA helicase DDX39B
+Macromolecule #9: THO complex subunit 4
-Experimental details
-Structure determination
Method | ![]() |
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Aggregation state | particle |
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Sample preparation
Concentration | 0.2 mg/mL |
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Buffer | pH: 7.9 |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: EMDB MAP EMDB ID: |
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Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.9 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 182534 |