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TitleStructural basis for clearing of ribosome collisions by the RQT complex.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 921, Year 2023
Publish dateFeb 17, 2023
AuthorsKatharina Best / Ken Ikeuchi / Lukas Kater / Daniel Best / Joanna Musial / Yoshitaka Matsuo / Otto Berninghausen / Thomas Becker / Toshifumi Inada / Roland Beckmann /
PubMed AbstractTranslation of aberrant messenger RNAs can cause stalling of ribosomes resulting in ribosomal collisions. Collided ribosomes are specifically recognized to initiate stress responses and quality ...Translation of aberrant messenger RNAs can cause stalling of ribosomes resulting in ribosomal collisions. Collided ribosomes are specifically recognized to initiate stress responses and quality control pathways. Ribosome-associated quality control facilitates the degradation of incomplete translation products and requires dissociation of the stalled ribosomes. A central event is therefore the splitting of collided ribosomes by the ribosome quality control trigger complex, RQT, by an unknown mechanism. Here we show that RQT requires accessible mRNA and the presence of a neighboring ribosome. Cryogenic electron microscopy of RQT-ribosome complexes reveals that RQT engages the 40S subunit of the lead ribosome and can switch between two conformations. We propose that the Ski2-like helicase 1 (Slh1) subunit of RQT applies a pulling force on the mRNA, causing destabilizing conformational changes of the small ribosomal subunit, ultimately resulting in subunit dissociation. Our findings provide conceptual framework for a helicase-driven ribosomal splitting mechanism.
External linksNat Commun / PubMed:36801861 / PubMed Central
MethodsEM (single particle)
Resolution2.4 - 4.8 Å
Structure data

14861

7zpq

EMDB-14861, PDB-7zpq:
Structure of the RQT-bound 80S ribosome from S. cerevisiae (C1)
Method: EM (single particle) / Resolution: 3.47 Å

14921

7zrs

EMDB-14921, PDB-7zrs:
Structure of the RQT-bound 80S ribosome from S. cerevisiae (C2) - composite map
Method: EM (single particle) / Resolution: 4.8 Å

14926

7zs5

EMDB-14926, PDB-7zs5:
Structure of 60S ribosomal subunit from S. cerevisiae with eIF6 and tRNA
Method: EM (single particle) / Resolution: 3.2 Å

14978

7zuw

EMDB-14978, PDB-7zuw:
Structure of RQT (C1) bound to the stalled ribosome in a disome unit from S. cerevisiae
Method: EM (single particle) / Resolution: 4.3 Å

14979

7zux

EMDB-14979, PDB-7zux:
Collided ribosome in a disome unit from S. cerevisiae
Method: EM (single particle) / Resolution: 2.5 Å

EMDB-15228: RQT-bound 80S ribosome from S. cerevisiae (C1, raw consensus map)
Method: EM (single particle) / Resolution: 2.4 Å

EMDB-16550: Structure of the RQT-bound 80S ribosome from S. cerevisiae (C2) - focused Refinement 80S
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-16551: Structure of the RQT-bound 80S ribosome from S. cerevisiae (C2) - focused Refinement RQT
Method: EM (single particle) / Resolution: 4.8 Å

EMDB-16552: Structure of the RQT-bound 80S ribosome from S. cerevisiae (C2) - composite map
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-16553: Structure of RQT (C1) bound to the stalled ribosome in a disome unit from S. cerevisiae - focused Refinement 80S
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-16554: Structure of RQT (C1) bound to the stalled ribosome in a disome unit from S. cerevisiae - focused Refinement RQT
Method: EM (single particle) / Resolution: 4.3 Å

EMDB-16571: Structure of the RQT-bound 80S ribosome from S. cerevisiae (C2) - consensus map
Method: EM (single particle) / Resolution: 2.9 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-UNK:
UNKNOWN

Source
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsRIBOSOME / collision / RNA binding / RQT / RQC / splitting / eIF6 / Tif6p

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