+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-14979 | ||||||||||||
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Title | Collided ribosome in a disome unit from S. cerevisiae | ||||||||||||
Map data | map of the collided ribosome in a disome unit | ||||||||||||
Sample |
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Keywords | collision / splitting / RQC / RQT / RIBOSOME | ||||||||||||
Function / homology | Function and homology information maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) / negative regulation of glucose mediated signaling pathway / negative regulation of translational frameshifting / positive regulation of translational fidelity / Protein methylation / RMTs methylate histone arginines / mTORC1-mediated signalling / Protein hydroxylation / ribosome-associated ubiquitin-dependent protein catabolic process / GDP-dissociation inhibitor activity ...maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) / negative regulation of glucose mediated signaling pathway / negative regulation of translational frameshifting / positive regulation of translational fidelity / Protein methylation / RMTs methylate histone arginines / mTORC1-mediated signalling / Protein hydroxylation / ribosome-associated ubiquitin-dependent protein catabolic process / GDP-dissociation inhibitor activity / nonfunctional rRNA decay / pre-mRNA 5'-splice site binding / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / preribosome, small subunit precursor / response to cycloheximide / mRNA destabilization / Major pathway of rRNA processing in the nucleolus and cytosol / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Formation of a pool of free 40S subunits / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / negative regulation of mRNA splicing, via spliceosome / L13a-mediated translational silencing of Ceruloplasmin expression / regulation of cellular amino acid metabolic process / preribosome, large subunit precursor / translational elongation / ribosomal large subunit export from nucleus / G-protein alpha-subunit binding / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / 90S preribosome / positive regulation of protein kinase activity / protein-RNA complex assembly / regulation of translational fidelity / ribosomal subunit export from nucleus / translation regulator activity / ribosomal small subunit export from nucleus / translational termination / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / DNA-(apurinic or apyrimidinic site) endonuclease activity / maturation of LSU-rRNA / cellular response to amino acid starvation / rescue of stalled ribosome / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosome assembly / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / maturation of SSU-rRNA / small-subunit processome / translational initiation / macroautophagy / protein kinase C binding / maintenance of translational fidelity / modification-dependent protein catabolic process / cytoplasmic stress granule / rRNA processing / protein tag activity / ribosome biogenesis / ribosome binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / 5S rRNA binding / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit / cytoplasmic translation / rRNA binding / negative regulation of translation / ribosome / protein ubiquitination / structural constituent of ribosome / translation / positive regulation of protein phosphorylation / G protein-coupled receptor signaling pathway / negative regulation of gene expression / response to antibiotic / mRNA binding / ubiquitin protein ligase binding / nucleolus / mitochondrion / RNA binding / zinc ion binding / nucleoplasm / nucleus / metal ion binding / cytoplasm / cytosol Similarity search - Function | ||||||||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.5 Å | ||||||||||||
Authors | Best KM / Ikeuchi K / Kater L / Best DM / Musial J / Matsuo Y / Berninghausen O / Becker T / Inada T / Beckmann R | ||||||||||||
Funding support | European Union, Germany, 3 items
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Citation | Journal: Nat Commun / Year: 2023 Title: Structural basis for clearing of ribosome collisions by the RQT complex. Authors: Katharina Best / Ken Ikeuchi / Lukas Kater / Daniel Best / Joanna Musial / Yoshitaka Matsuo / Otto Berninghausen / Thomas Becker / Toshifumi Inada / Roland Beckmann / Abstract: Translation of aberrant messenger RNAs can cause stalling of ribosomes resulting in ribosomal collisions. Collided ribosomes are specifically recognized to initiate stress responses and quality ...Translation of aberrant messenger RNAs can cause stalling of ribosomes resulting in ribosomal collisions. Collided ribosomes are specifically recognized to initiate stress responses and quality control pathways. Ribosome-associated quality control facilitates the degradation of incomplete translation products and requires dissociation of the stalled ribosomes. A central event is therefore the splitting of collided ribosomes by the ribosome quality control trigger complex, RQT, by an unknown mechanism. Here we show that RQT requires accessible mRNA and the presence of a neighboring ribosome. Cryogenic electron microscopy of RQT-ribosome complexes reveals that RQT engages the 40S subunit of the lead ribosome and can switch between two conformations. We propose that the Ski2-like helicase 1 (Slh1) subunit of RQT applies a pulling force on the mRNA, causing destabilizing conformational changes of the small ribosomal subunit, ultimately resulting in subunit dissociation. Our findings provide conceptual framework for a helicase-driven ribosomal splitting mechanism. #1: Journal: Acta Crystallogr D Struct Biol / Year: 2018 Title: Real-space refinement in PHENIX for cryo-EM and crystallography. Authors: Afonine PV / Poon BK / Read RJ / Sobolev OV / Terwilliger TC / Urzhumtsev A / Adams PD | ||||||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_14979.map.gz | 45.9 MB | EMDB map data format | |
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Header (meta data) | emd-14979-v30.xml emd-14979.xml | 112.8 KB 112.8 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_14979_fsc.xml | 18.4 KB | Display | FSC data file |
Images | emd_14979.png | 118.2 KB | ||
Masks | emd_14979_msk_1.map | 669.9 MB | Mask map | |
Filedesc metadata | emd-14979.cif.gz | 18.3 KB | ||
Others | emd_14979_half_map_1.map.gz emd_14979_half_map_2.map.gz | 448.8 MB 448.8 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-14979 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-14979 | HTTPS FTP |
-Validation report
Summary document | emd_14979_validation.pdf.gz | 839 KB | Display | EMDB validaton report |
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Full document | emd_14979_full_validation.pdf.gz | 838.5 KB | Display | |
Data in XML | emd_14979_validation.xml.gz | 28.3 KB | Display | |
Data in CIF | emd_14979_validation.cif.gz | 37.7 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-14979 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-14979 | HTTPS FTP |
-Related structure data
Related structure data | 7zuxMC 7zpqC 7zrsC 7zs5C 7zuwC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_14979.map.gz / Format: CCP4 / Size: 669.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Annotation | map of the collided ribosome in a disome unit | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.045 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
File | emd_14979_msk_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: halfmap 1
File | emd_14979_half_map_1.map | ||||||||||||
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Annotation | halfmap 1 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: halfmap 2
File | emd_14979_half_map_2.map | ||||||||||||
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Annotation | halfmap 2 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
+Entire : collided ribosome of a disome unit
+Supramolecule #1: collided ribosome of a disome unit
+Supramolecule #2: 40S ribosomal subunit
+Supramolecule #3: 60S ribosomal subunit
+Macromolecule #1: 18S ribosomal RNA
+Macromolecule #2: 5.8S ribosomal RNA
+Macromolecule #3: 5S ribosomal RNA
+Macromolecule #4: 25S ribosomal RNA
+Macromolecule #5: A/P tRNA
+Macromolecule #6: P/E tRNA
+Macromolecule #7: 40S ribosomal protein S0-A
+Macromolecule #8: 40S ribosomal protein S1-A
+Macromolecule #9: 40S ribosomal protein S2
+Macromolecule #10: 40S ribosomal protein S3
+Macromolecule #11: 40S ribosomal protein S4-A
+Macromolecule #12: 40S ribosomal protein S5
+Macromolecule #13: 40S ribosomal protein S6-A
+Macromolecule #14: 40S ribosomal protein S7-A
+Macromolecule #15: 40S ribosomal protein S8-B
+Macromolecule #16: 40S ribosomal protein S9-A
+Macromolecule #17: 40S ribosomal protein S10-A
+Macromolecule #18: 40S ribosomal protein S11-A
+Macromolecule #19: 40S ribosomal protein S12
+Macromolecule #20: 40S ribosomal protein S13
+Macromolecule #21: 40S ribosomal protein S14-B
+Macromolecule #22: 40S ribosomal protein S15
+Macromolecule #23: 40S ribosomal protein S16-A
+Macromolecule #24: 40S ribosomal protein S17-A
+Macromolecule #25: 40S ribosomal protein S18-A
+Macromolecule #26: 40S ribosomal protein S19-A
+Macromolecule #27: 40S ribosomal protein S20
+Macromolecule #28: 40S ribosomal protein S21-A
+Macromolecule #29: 40S ribosomal protein S22-A
+Macromolecule #30: 40S ribosomal protein S23-A
+Macromolecule #31: 40S ribosomal protein S24-A
+Macromolecule #32: 40S ribosomal protein S25-A
+Macromolecule #33: 40S ribosomal protein S26-B
+Macromolecule #34: 40S ribosomal protein S27-A
+Macromolecule #35: 40S ribosomal protein S28-A
+Macromolecule #36: 40S ribosomal protein S29-A
+Macromolecule #37: 40S ribosomal protein S30-A
+Macromolecule #38: Ubiquitin
+Macromolecule #39: Guanine nucleotide-binding protein subunit beta-like protein
+Macromolecule #40: 60S ribosomal protein L2-A
+Macromolecule #41: 60S ribosomal protein L3
+Macromolecule #42: 60S ribosomal protein L4-A
+Macromolecule #43: 60S ribosomal protein L5
+Macromolecule #44: 60S ribosomal protein L6-B
+Macromolecule #45: 60S ribosomal protein L7-A
+Macromolecule #46: 60S ribosomal protein L8-A
+Macromolecule #47: 60S ribosomal protein L9-A
+Macromolecule #48: 60S ribosomal protein L10
+Macromolecule #49: 60S ribosomal protein L11-B
+Macromolecule #50: 60S ribosomal protein L13-A
+Macromolecule #51: 60S ribosomal protein L14-A
+Macromolecule #52: 60S ribosomal protein L15-A
+Macromolecule #53: 60S ribosomal protein L16-A
+Macromolecule #54: 60S ribosomal protein L17-A
+Macromolecule #55: 60S ribosomal protein L18-A
+Macromolecule #56: 60S ribosomal protein L19-A
+Macromolecule #57: 60S ribosomal protein L20-A
+Macromolecule #58: 60S ribosomal protein L21-A
+Macromolecule #59: 60S ribosomal protein L22-A
+Macromolecule #60: 60S ribosomal protein L23-A
+Macromolecule #61: 60S ribosomal protein L24-A
+Macromolecule #62: 60S ribosomal protein L25
+Macromolecule #63: 60S ribosomal protein L26-A
+Macromolecule #64: 60S ribosomal protein L27-A
+Macromolecule #65: 60S ribosomal protein L28
+Macromolecule #66: 60S ribosomal protein L29
+Macromolecule #67: 60S ribosomal protein L30
+Macromolecule #68: 60S ribosomal protein L31-A
+Macromolecule #69: 60S ribosomal protein L32
+Macromolecule #70: 60S ribosomal protein L33-A
+Macromolecule #71: 60S ribosomal protein L34-A
+Macromolecule #72: 60S ribosomal protein L35-A
+Macromolecule #73: 60S ribosomal protein L36-A
+Macromolecule #74: 60S ribosomal protein L37-A
+Macromolecule #75: 60S ribosomal protein L38
+Macromolecule #76: 60S ribosomal protein L39
+Macromolecule #77: 60S ribosomal protein L40-A
+Macromolecule #78: 60S ribosomal protein L41-A
+Macromolecule #79: 60S ribosomal protein L42-A
+Macromolecule #80: 60S ribosomal protein L43-A
+Macromolecule #81: MAGNESIUM ION
+Macromolecule #82: ZINC ION
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 44.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 0.5 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |