EMDB-14978: Structure of RQT (C1) bound to the stalled ribosome in a disome u...

Basic information

Entry

Database: EMDB / ID: EMD-14978

• Title: Structure of RQT (C1) bound to the stalled ribosome in a disome unit from S. cerevisiae
• Map data: locally refined and filtered composite map ligand and ribosome

Sample

• Complex: RQC trigger complex bound to the stalled ribosome of a disome unit
  • Complex: ribosome
    • RNA: x 5 types
    • Protein or peptide: x 74 types
  • Complex: RQC trigger complex with Slh1, Cue3, Rqt4
    • Protein or peptide: x 3 types
• Ligand: x 2 types

Function / homology

Function:

ribosomal subunit / negative regulation of glucose mediated signaling pathway / negative regulation of translational frameshifting / Negative regulators of DDX58/IFIH1 signaling / ribosome disassembly / mTORC1-mediated signalling / Protein hydroxylation / ribosome-associated ubiquitin-dependent protein catabolic process / GDP-dissociation inhibitor activity / pre-mRNA 5'-splice site binding / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / response to cycloheximide / mRNA destabilization / Major pathway of rRNA processing in the nucleolus and cytosol / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Formation of a pool of free 40S subunits / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / negative regulation of mRNA splicing, via spliceosome / L13a-mediated translational silencing of Ceruloplasmin expression / preribosome, large subunit precursor / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit export from nucleus / G-protein alpha-subunit binding / positive regulation of protein kinase activity / protein-RNA complex assembly / regulation of translational fidelity / Ub-specific processing proteases / translation regulator activity / ribosomal subunit export from nucleus / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / cellular response to amino acid starvation / rescue of stalled ribosome / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / 90S preribosome / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / helicase activity / maturation of LSU-rRNA / ribosomal large subunit biogenesis / ubiquitin binding / maturation of SSU-rRNA / small-subunit processome / positive regulation of apoptotic signaling pathway / protein kinase C binding / macroautophagy / maintenance of translational fidelity / ribosomal large subunit assembly / cytoplasmic stress granule / modification-dependent protein catabolic process / rRNA processing / protein tag activity / ribosome biogenesis / ribosome binding / regulation of translation / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / 5S rRNA binding / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / defense response to virus / cytosolic large ribosomal subunit / cytoplasmic translation / RNA helicase activity / transcription coactivator activity / rRNA binding / negative regulation of translation / ribosome / protein ubiquitination / RNA helicase / structural constituent of ribosome / ribonucleoprotein complex / translation / positive regulation of protein phosphorylation / G protein-coupled receptor signaling pathway / negative regulation of gene expression / response to antibiotic / mRNA binding / ubiquitin protein ligase binding / nucleolus / positive regulation of DNA-templated transcription / ATP hydrolysis activity / mitochondrion / RNA binding / zinc ion binding / nucleoplasm / ATP binding / nucleus / metal ion binding / cytoplasm / cytosol

Domain/homology:

CUE domain-containing protein 3, CUE domain / Zinc finger, C2HC5-type / Activating signal cointegrator 1-like / Putative zinc finger motif, C2HC5-type / CUE domain / Domain that may be involved in binding ubiquitin-conjugating enzymes (UBCs) / Ubiquitin system component CUE / CUE domain profile. / Sec63 Brl domain / Sec63 domain / Sec63 Brl domain / : / Ribosomal protein S26e signature. / : / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / Ribosomal protein S12e signature. / Ribosomal protein S12e / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S2, eukaryotic / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein L29e / Ribosomal L29e protein family / 40S Ribosomal protein S10 / S27a-like superfamily / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / Ribosomal protein S2, eukaryotic/archaeal / : / Ribosomal protein S17e, conserved site / Ribosomal protein S17e signature. / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S8e subdomain, eukaryotes / Ribosomal protein S30 / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein S7e signature. / Ribosomal protein L44e signature. / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein L10e / Ribosomal protein L13e / Ribosomal protein L13e / Ribosomal protein L19, eukaryotic / Ribosomal protein S19e / Ribosomal protein S3Ae, conserved site / Ribosomal protein S19e / Ribosomal protein S3Ae signature. / Ribosomal_S19e / Ribosomal protein S27e signature. / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal protein S4e signature. / 40S ribosomal protein S4, C-terminal domain / 40S ribosomal protein S4 C-terminus / : / Ribosomal protein S19A/S15e / Ribosomal protein S8e, conserved site / Ribosomal protein S8e signature. / Ribosomal protein L24e, conserved site / Ribosomal protein L24e signature. / Ribosomal protein L44e / Ribosomal protein L19/L19e conserved site / Ribosomal protein L44 / Ribosomal protein L19e signature. / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal S17 / Ribosomal protein L6e signature. / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a

Component:

RPS5 isoform 1 / RPS22A isoform 1 / RPS31 isoform 1 / RPL38 isoform 1 / RPL10 isoform 1 / RPS29A isoform 1 / RPS20 isoform 1 / RPS2 isoform 1 / 60S ribosomal protein L29 / RPL24A isoform 1 / RPL11B isoform 1 / 40S ribosomal protein S25 / 40S ribosomal protein S26 / RPL5 isoform 1 / RPL32 isoform 1 / 40S ribosomal protein S3 / RPL4A isoform 1 / Large ribosomal subunit protein uL3 / RPS15 isoform 1 / RPS28A isoform 1 / 40S ribosomal protein S12 / Ribosomal protein L15 / CUE3 isoform 1 / 60S ribosomal protein L8 / RPL9A isoform 1 / 60S ribosomal protein L41 / Small ribosomal subunit protein uS2A / Small ribosomal subunit protein uS4A / Large ribosomal subunit protein uL15 / Small ribosomal subunit protein eS17A / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein uL30A / Large ribosomal subunit protein eL6B / Large ribosomal subunit protein uL22A / Large ribosomal subunit protein uL24A / Large ribosomal subunit protein eL33A / Large ribosomal subunit protein eL36A / Large ribosomal subunit protein eL22A / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein eS19A / Small ribosomal subunit protein eS21A / Large ribosomal subunit protein eL27A / Large ribosomal subunit protein eL31A / Ubiquitin-ribosomal protein eL40A fusion protein / Large ribosomal subunit protein eL20A / Large ribosomal subunit protein eL43A / Large ribosomal subunit protein eL42A / Small ribosomal subunit protein uS12A / Small ribosomal subunit protein eS24A / Small ribosomal subunit protein eS30A / Small ribosomal subunit protein eS4A / Small ribosomal subunit protein eS6A / Small ribosomal subunit protein eS8B / Large ribosomal subunit protein uL14A / Large ribosomal subunit protein uL2A / Small ribosomal subunit protein uS17A / Large ribosomal subunit protein eL18A / Small ribosomal subunit protein uS9A / Small ribosomal subunit protein uS13A / Large ribosomal subunit protein eL19A / Large ribosomal subunit protein uL29A / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein uL13A / Small ribosomal subunit protein eS7A / Small ribosomal subunit protein eS1A / Small ribosomal subunit protein eS27A / Large ribosomal subunit protein eL14A / RQC trigger complex subunit RQT4 / Small ribosomal subunit protein RACK1 / Small ribosomal subunit protein uS11B / Large ribosomal subunit protein eL37A / RQC trigger complex helicase SLH1 / Large ribosomal subunit protein eL34A / Large ribosomal subunit protein eL21A / Small ribosomal subunit protein eS10A / Large ribosomal subunit protein eL13A

• Biological species: Saccharomyces cerevisiae (brewer's yeast) / baker's yeast (brewer's yeast)
• Method: single particle reconstruction / cryo EM / Resolution: 4.3 Å
• Authors: Best KM / Ikeuchi K / Kater L / Best DM / Musial J / Matsuo Y / Berninghausen O / Becker T / Inada T / Beckmann R

Funding support

European Union, Germany, 3 items

Organization	Grant number	Country
European Research Council (ERC)	885711	European Union
German Research Foundation (DFG)	BE 1814/15-1	Germany
German Research Foundation (DFG)	RTG1721	Germany

Citation

Journal: Nat Commun / Year: 2023
Title: Structural basis for clearing of ribosome collisions by the RQT complex.
Authors: Katharina Best / Ken Ikeuchi / Lukas Kater / Daniel Best / Joanna Musial / Yoshitaka Matsuo / Otto Berninghausen / Thomas Becker / Toshifumi Inada / Roland Beckmann /
Abstract: Translation of aberrant messenger RNAs can cause stalling of ribosomes resulting in ribosomal collisions. Collided ribosomes are specifically recognized to initiate stress responses and quality control pathways. Ribosome-associated quality control facilitates the degradation of incomplete translation products and requires dissociation of the stalled ribosomes. A central event is therefore the splitting of collided ribosomes by the ribosome quality control trigger complex, RQT, by an unknown mechanism. Here we show that RQT requires accessible mRNA and the presence of a neighboring ribosome. Cryogenic electron microscopy of RQT-ribosome complexes reveals that RQT engages the 40S subunit of the lead ribosome and can switch between two conformations. We propose that the Ski2-like helicase 1 (Slh1) subunit of RQT applies a pulling force on the mRNA, causing destabilizing conformational changes of the small ribosomal subunit, ultimately resulting in subunit dissociation. Our findings provide conceptual framework for a helicase-driven ribosomal splitting mechanism.

#1: Journal: Acta Crystallogr D Struct Biol / Year: 2018
Title: Real-space refinement in PHENIX for cryo-EM and crystallography.
Authors: Afonine PV / Poon BK / Read RJ / Sobolev OV / Terwilliger TC / Urzhumtsev A / Adams PD

History

Deposition	May 13, 2022	-
Header (metadata) release	Feb 22, 2023	-
Map release	Feb 22, 2023	-
Update	Mar 1, 2023	-
Current status	Mar 1, 2023	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_14978.map.gz / Format: CCP4 / Size: 669.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: locally refined and filtered composite map ligand and ribosome
• Voxel size: X=Y=Z: 1.045 Å

Density

Contour Level	By AUTHOR: 0.3
Minimum - Maximum	-1.0151854 - 4.3832006
Average (Standard dev.)	0.019519664 (±0.109397456)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 560 560 560 Spacing 560 560 560
Cell	A=B=C: 585.19995 Å α=β=γ: 90.0 °

Supplemental data

Mask #1

• File: emd_14978_msk_1.map

Mask #2

• File: emd_14978_msk_2.map

Additional map: locally refined and filtered map of the ribosome

• File: emd_14978_additional_1.map
• Annotation: locally refined and filtered map of the ribosome

Additional map: halfmap 2 of the locally refined ligand

• File: emd_14978_additional_2.map
• Annotation: halfmap 2 of the locally refined ligand

Additional map: map of the locally refined and filtered ligand

• File: emd_14978_additional_3.map
• Annotation: map of the locally refined and filtered ligand

Additional map: halfmap 1 of the locally refined ligand

• File: emd_14978_additional_4.map
• Annotation: halfmap 1 of the locally refined ligand

Additional map: composite map of stalled and collided ribosome

• File: emd_14978_additional_5.map
• Annotation: composite map of stalled and collided ribosome

Half map: halfmap 2 of the ribosome

• File: emd_14978_half_map_1.map
• Annotation: halfmap 2 of the ribosome

Half map: halfmap 1 of the ribosome

• File: emd_14978_half_map_2.map
• Annotation: halfmap 1 of the ribosome

Sample components

Entire : RQC trigger complex bound to the stalled ribosome of a disome unit

• Entire: Name: RQC trigger complex bound to the stalled ribosome of a disome unit

Components

• Complex: RQC trigger complex bound to the stalled ribosome of a disome unit
  • Complex: ribosome
    • RNA: 18S ribosomal RNA
    • RNA: 5.8S ribosomal RNA
    • RNA: 5S ribosomal RNA
    • RNA: 25S ribosomal RNA
    • RNA: tRNA
    • Protein or peptide: 40S ribosomal protein S0-A
    • Protein or peptide: 40S ribosomal protein S1-A
    • Protein or peptide: RPS2 isoform 1
    • Protein or peptide: RPS3 isoform 1
    • Protein or peptide: 40S ribosomal protein S4-A
    • Protein or peptide: Rps5p
    • Protein or peptide: 40S ribosomal protein S6-A
    • Protein or peptide: 40S ribosomal protein S7-A
    • Protein or peptide: 40S ribosomal protein S8-B
    • Protein or peptide: 40S ribosomal protein S9-A
    • Protein or peptide: 40S ribosomal protein S10-A
    • Protein or peptide: 40S ribosomal protein S11-A
    • Protein or peptide: 40S ribosomal protein S12
    • Protein or peptide: 40S ribosomal protein S13
    • Protein or peptide: 40S ribosomal protein S14-B
    • Protein or peptide: RPS15 isoform 1
    • Protein or peptide: 40S ribosomal protein S16-A
    • Protein or peptide: 40S ribosomal protein S17-A
    • Protein or peptide: 40S ribosomal protein S18-A
    • Protein or peptide: 40S ribosomal protein S19-A
    • Protein or peptide: RPS20 isoform 1
    • Protein or peptide: 40S ribosomal protein S21-A
    • Protein or peptide: RPS22A isoform 1
    • Protein or peptide: 40S ribosomal protein S23-A
    • Protein or peptide: 40S ribosomal protein S24-A
    • Protein or peptide: 40S ribosomal protein S25
    • Protein or peptide: 40S ribosomal protein S26
    • Protein or peptide: 40S ribosomal protein S27-A
    • Protein or peptide: RPS28A isoform 1
    • Protein or peptide: RPS29A isoform 1
    • Protein or peptide: 40S ribosomal protein S30-A
    • Protein or peptide: RPS31 isoform 1
    • Protein or peptide: Guanine nucleotide-binding protein subunit beta-like protein
    • Protein or peptide: 60S ribosomal protein L2-A
    • Protein or peptide: 60S ribosomal protein L3
    • Protein or peptide: RPL4A isoform 1
    • Protein or peptide: RPL5 isoform 1
    • Protein or peptide: 60S ribosomal protein L6-B
    • Protein or peptide: 60S ribosomal protein L7-A
    • Protein or peptide: 60S ribosomal protein L8
    • Protein or peptide: RPL9A isoform 1
    • Protein or peptide: RPL10 isoform 1
    • Protein or peptide: RPL11B isoform 1
    • Protein or peptide: 60S ribosomal protein L13-A
    • Protein or peptide: 60S ribosomal protein L14-A
    • Protein or peptide: Ribosomal protein L15
    • Protein or peptide: 60S ribosomal protein L16-A
    • Protein or peptide: 60S ribosomal protein L17-A
    • Protein or peptide: 60S ribosomal protein L18-A
    • Protein or peptide: 60S ribosomal protein L19-A
    • Protein or peptide: 60S ribosomal protein L20-A
    • Protein or peptide: 60S ribosomal protein L21-A
    • Protein or peptide: 60S ribosomal protein L22-A
    • Protein or peptide: 60S ribosomal protein L23-A
    • Protein or peptide: RPL24A isoform 1
    • Protein or peptide: 60S ribosomal protein L25
    • Protein or peptide: 60S ribosomal protein L26-A
    • Protein or peptide: 60S ribosomal protein L27-A
    • Protein or peptide: 60S ribosomal protein L28
    • Protein or peptide: 60S ribosomal protein L29
    • Protein or peptide: 60S ribosomal protein L30
    • Protein or peptide: 60S ribosomal protein L31-A
    • Protein or peptide: RPL32 isoform 1
    • Protein or peptide: 60S ribosomal protein L33-A
    • Protein or peptide: 60S ribosomal protein L34-A
    • Protein or peptide: 60S ribosomal protein L35-A
    • Protein or peptide: 60S ribosomal protein L36-A
    • Protein or peptide: 60S ribosomal protein L37-A
    • Protein or peptide: RPL38 isoform 1
    • Protein or peptide: 60S ribosomal protein L39
    • Protein or peptide: 60S ribosomal protein L40-A
    • Protein or peptide: 60S ribosomal protein L41
    • Protein or peptide: 60S ribosomal protein L42-A
    • Protein or peptide: 60S ribosomal protein L43-A
  • Complex: RQC trigger complex with Slh1, Cue3, Rqt4
    • Protein or peptide: RQC trigger complex helicase SLH1
    • Protein or peptide: CUE3 isoform 1
    • Protein or peptide: RQC trigger complex subunit RQT4
• Ligand: MAGNESIUM ION
• Ligand: ZINC ION

Supramolecule #1: RQC trigger complex bound to the stalled ribosome of a disome unit

• Supramolecule: Name: RQC trigger complex bound to the stalled ribosome of a disome unit; type: complex / ID: 1 / Chimera: Yes / Parent: 0 / Macromolecule list: #1-#82
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)

Supramolecule #2: ribosome

• Supramolecule: Name: ribosome / type: complex / ID: 2 / Chimera: Yes / Parent: 1 / Macromolecule list: #1-#79
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)

Supramolecule #3: RQC trigger complex with Slh1, Cue3, Rqt4

• Supramolecule: Name: RQC trigger complex with Slh1, Cue3, Rqt4 / type: complex / ID: 3 / Chimera: Yes / Parent: 1 / Macromolecule list: #80-#82
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)

Macromolecule #1: 18S ribosomal RNA

• Macromolecule: Name: 18S ribosomal RNA / type: rna / ID: 1 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 579.761938 KDa

Sequence

String:

UAUCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUAUACA GUGAAACUG CGAAUGGCUC AUUAAAUCAG UUAUCGUUUA UUUGAUAGUU CCUUUACUAC AUGGUAUAAC UGUGGUAAUU C UAGAGCUA AUACAUGCUU AAAAUCUCGA CCCUUUGGAA GAGAUGUAUU UAUUAGAUAA AAAAUCAAUG UCUUCGGACU CU UUGAUGA UUCAUAAUAA CUUUUCGAAU CGCAUGGCCU UGUGCUGGCG AUGGUUCAUU CAAAUUUCUG CCCUAUCAAC UUU CGAUGG UAGGAUAGUG GCCUACCAUG GUUUCAACGG GUAACGGGGA AUAAGGGUUC GAUUCCGGAG AGGGAGCCUG AGAA ACGGC UACCACAUCC AAGGAAGGCA GCAGGCGCGC AAAUUACCCA AUCCUAAUUC AGGGAGGUAG UGACAAUAAA UAACG AUAC AGGGCCCAUU CGGGUCUUGU AAUUGGAAUG AGUACAAUGU AAAUACCUUA ACGAGGAACA AUUGGAGGGC AAGUCU GGU GCCAGCAGCC GCGGUAAUUC CAGCUCCAAU AGCGUAUAUU AAAGUUGUUG CAGUUAAAAA GCUCGUAGUU GAACUUU GG GCCCGGUUGG CCGGUCCGAU UUUUUCGUGU ACUGGAUUUC CAACGGGGCC UUUCCUUCUG GCUAACCUUG AGUCCUUG U GGCUCUUGGC GAACCAGGAC UUUUACUUUG AAAAAAUUAG AGUGUUCAAA GCAGGCGUAU UGCUCGAAUA UAUUAGCAU GGAAUAAUAG AAUAGGACGU UUGGUUCUAU UUUGUUGGUU UCUAGGACCA UCGUAAUGAU UAAUAGGGAC GGUCGGGGGC AUCAGUAUU CAAUUGUCAG AGGUGAAAUU CUUGGAUUUA UUGAAGACUA ACUACUGCGA AAGCAUUUGC CAAGGACGUU U UCAUUAAU CAAGAACGAA AGUUAGGGGA UCGAAGAUGA UCAGAUACCG UCGUAGUCUU AACCAUAAAC UAUGCCGACU AG GGAUCGG GUGGUGUUUU UUUAAUGACC CACUCGGCAC CUUACGAGAA AUCAAAGUCU UUGGGUUCUG GGGGGAGUAU GGU CGCAAG GCUGAAACUU AAAGGAAUUG ACGGAAGGGC ACCACCAGGA GUGGAGCCUG CGGCUUAAUU UGACUCAACA CGGG GAAAC UCACCAGGUC CAGACACAAU AAGGAUUGAC AGAUUGAGAG CUCUUUCUUG AUUUUGUGGG UGGUGGUGCA UGGCC GUUC UUAGUUGGUG GAGUGAUUUG UCUGCUUAAU UGCGAUAACG AACGAGACCU UAACCUACUA AAUAGUGGUG CUAGCA UUU GCUGGUUAUC CACUUCUUAG AGGGACUAUC GGUUUCAAGC CGAUGGAAGU UUGAGGCAAU AACAGGUCUG UGAUGCC CU UAGACGUUCU GGGCCGCACG CGCGCUACAC UGACGGAGCC AGCGAGUCUA ACCUUGGCCG AGAGGUCUUG GUAAUCUU G UGAAACUCCG UCGUGCUGGG GAUAGAGCAU UGUAAUUAUU GCUCUUCAAC GAGGAAUUCC UAGUAAGCGC AAGUCAUCA GCUUGCGUUG AUUACGUCCC UGCCCUUUGU ACACACCGCC CGUCGCUAGU ACCGAUUGAA UGGCUUAGUG AGGCCUCAGG AUCUGCUUA GAGAAGGGGG CAACUCCAUC UCAGAGCGGA GAAUUUGGAC AAACUUGGUC AUUUAGAGGA ACUAAAAGUC G UAACAAGG UUUCCGUAGG UGAACCUGCG GAAGGAUCAU UA

Macromolecule #2: 5.8S ribosomal RNA

• Macromolecule: Name: 5.8S ribosomal RNA / type: rna / ID: 2 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 50.682922 KDa

Sequence

String:

AAACUUUCAA CAACGGAUCU CUUGGUUCUC GCAUCGAUGA AGAACGCAGC GAAAUGCGAU ACGUAAUGUG AAUUGCAGAA UUCCGUGAA UCAUCGAAUC UUUGAACGCA CAUUGCGCCC CUUGGUAUUC CAGGGGGCAU GCCUGUUUGA GCGUCAUUU

Macromolecule #3: 5S ribosomal RNA

• Macromolecule: Name: 5S ribosomal RNA / type: rna / ID: 3 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 38.951105 KDa

Sequence

String:

GGUUGCGGCC AUAUCUACCA GAAAGCACCG UUUCCCGUCC GAUCAACUGU AGUUAAGCUG GUAAGAGCCU GACCGAGUAG UGUAGUGGG UGACCAUACG CGAAACUCAG GUGCUGCAAU CU

Macromolecule #4: 25S ribosomal RNA

• Macromolecule: Name: 25S ribosomal RNA / type: rna / ID: 4 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 1.0974938749999998 MDa

Sequence

String:

GUUUGACCUC AAAUCAGGUA GGAGUACCCG CUGAACUUAA GCAUAUCAAU AAGCGGAGGA AAAGAAACCA ACCGGGAUUG CCUUAGUAA CGGCGAGUGA AGCGGCAAAA GCUCAAAUUU GAAAUCUGGU ACCUUCGGUG CCCGAGUUGU AAUUUGGAGA G GGCAACUU UGGGGCCGUU CCUUGUCUAU GUUCCUUGGA ACAGGACGUC AUAGAGGGUG AGAAUCCCGU GUGGCGAGGA GU GCGGUUC UUUGUAAAGU GCCUUCGAAG AGUCGAGUUG UUUGGGAAUG CAGCUCUAAG UGGGUGGUAA AUUCCAUCUA AAG CUAAAU AUUGGCGAGA GACCGAUAGC GAACAAGUAC AGUGAUGGAA AGAUGAAAAG AACUUUGAAA AGAGAGUGAA AAAG UACGU GAAAUUGUUG AAAGGGAAGG GCAUUUGAUC AGACAUGGUG UUUUGUGCCC UCUGCUCCUU GUGGGUAGGG GAAUC UCGC AUUUCACUGG GCCAGCAUCA GUUUUGGUGG CAGGAUAAAU CCAUAGGAAU GUAGCUUGCC UCGGUAAGUA UUAUAG CCU GUGGGAAUAC UGCCAGCUGG GACUGAGGAC UGCGACGUAA GUCAAGGAUG CUGGCAUAAU GGUUAUAUGC CGCCCGU CU UGAAACACGG ACCAAGGAGU CUAACGUCUA UGCGAGUGUU UGGGUGUAAA ACCCAUACGC GUAAUGAAAG UGAACGUA G GUUGGGGCCU CGCAAGAGGU GCACAAUCGA CCGAUCCUGA UGUCUUCGGA UGGAUUUGAG UAAGAGCAUA GCUGUUGGG ACCCGAAAGA UGGUGAACUA UGCCUGAAUA GGGUGAAGCC AGAGGAAACU CUGGUGGAGG CUCGUAGCGG UUCUGACGUG CAAAUCGAU CGUCGAAUUU GGGUAUAGGG GCGAAAGACU AAUCGAACCA UCUAGUAGCU GGUUCCUGCC GAAGUUUCCC U CAGGAUAG CAGAAGCUCG UAUCAGUUUU AUGAGGUAAA GCGAAUGAUU AGAGGUUCCG GGGUCGAAAU GACCUUGACC UA UUCUCAA ACUUUAAAUA UGUAAGAAGU CCUUGUUACU UAAUUGAACG UGGACAUUUG AAUGAAGAGC UUUUAGUGGG CCA UUUUUG GUAAGCAGAA CUGGCGAUGC GGGAUGAACC GAACGUAGAG UUAAGGUGCC GGAAUACACG CUCAUCAGAC ACCA CAAAA GGUGUUAGUU CAUCUAGACA GCCGGACGGU GGCCAUGGAA GUCGGAAUCC GCUAAGGAGU GUGUAACAAC UCACC GGCC GAAUGAACUA GCCCUGAAAA UGGAUGGCGC UCAAGCGUGU UACCUAUACU CUACCGUCAG GGUUGAUAUG AUGCCC UGA CGAGUAGGCA GGCGUGGAGG UCAGUGACGA AGCCUAGACC GUAAGGUCGG GUCGAACGGC CUCUAGUGCA GAUCUUG GU GGUAGUAGCA AAUAUUCAAA UGAGAACUUU GAAGACUGAA GUGGGGAAAG GUUCCACGUC AACAGCAGUU GGACGUGG G UUAGUCGAUC CUAAGAGAUG GGGAAGCUCC GUUUCAAAGG CCUGAUUUUA UGCAGGCCAC CAUCGAAAGG GAAUCCGGU UAAGAUUCCG GAACCUGGAU AUGGAUUCUU CACGGUAACG UAACUGAAUG UGGAGACGUC GGCGCGAGCC CUGGGAGGAG UUAUCUUUU CUUCUUAACA GCUUAUCACC CCGGAAUUGG UUUAUCCGGA GAUGGGGUCU UAUGGCUGGA AGAGGCCAGC A CCUUUGCU GGCUCCGGUG CGCUUGUGAC GGCCCGUGAA AAUCCACAGG AAGGAAUAGU UUUCAUGCCA GGUCGUACUG AU AACCGCA GCAGGUCUCC AAGGUGAACA GCCUCUAGUU GAUAGAAUAA UGUAGAUAAG GGAAGUCGGC AAAAUAGAUC CGU AACUUC GGGAUAAGGA UUGGCUCUAA GGGUCGGGUA GUGAGGGCCU UGGUCAGACG CAGCGGGCGU GCUUGUGGAC UGCU UGGUG GGGCUUGCUC UGCUAGGCGG ACUACUUGCG UGCCUUGUUG UAGACGGCCU UGGUAGGUCU CUUGUAGACC GUCGC UUGC UACAAUUAAC GAUCAACUUA GAACUGGUAC GGACAAGGGG AAUCUGACUG UCUAAUUAAA ACAUAGCAUU GCGAUG GUC AGAAAGUGAU GUUGACGCAA UGUGAUUUCU GCCCAGUGCU CUGAAUGUCA AAGUGAAGAA AUUCAACCAA GCGCGGG UA AACGGCGGGA GUAACUAUGA CUCUCUUAAG GUAGCCAAAU GCCUCGUCAU CUAAUUAGUG ACGCGCAUGA AUGGAUUA A CGAGAUUCCC ACUGUCCCUA UCUACUAUCU AGCGAAACCA CAGCCAAGGG AACGGGCUUG GCAGAAUCAG CGGGGAAAG AAGACCCUGU UGAGCUUGAC UCUAGUUUGA CAUUGUGAAG AGACAUAGAG GGUGUAGAAU AAGUGGGAGC UUCGGCGCCA GUGAAAUAC CACUACCUUU AUAGUUUCUU UACUUAUUCA AUGAAGCGGA GCUGGAAUUC AUUUUCCACG UUCUAGCAUU C AAGGUCCC AUUCGGGGCU GAUCCGGGUU GAAGACAUUG UCAGGUGGGG AGUUUGGCUG GGGCGGCACA UCUGUUAAAC GA UAACGCA GAUGUCCUAA GGGGGGCUCA UGGAGAACAG AAAUCUCCAG UAGAACAAAA GGGUAAAAGC CCCCUUGAUU UUG AUUUUC AGUGUGAAUA CAAACCAUGA AAGUGUGGCC UAUCGAUCCU UUAGUCCCUC GGAAUUUGAG GCUAGAGGUG CCAG AAAAG UUACCACAGG GAUAACUGGC UUGUGGCAGU CAAGCGUUCA UAGCGACAUU GCUUUUUGAU UCUUCGAUGU CGGCU CUUC CUAUCAUACC GAAGCAGAAU UCGGUAAGCG UUGGAUUGUU CACCCACUAA UAGGGAACGU GAGCUGGGUU UAGACC GUC GUGAGACAGG UUAGUUUUAC CCUACUGAUG AAUGUUACCG CAAUAGUAAU UGAACUUAGU ACGAGAGGAA CAGUUCA UU CGGAUAAUUG GUUUUUGCGG CUGUCUGAUC AGGCAUUGCC GCGAAGCUAC CAUCCGCUGG AUUAUGGCUG AACGCCUC U AAGUCAGAAU CCAUGCUAGA ACGCGGUGAU UUCUUUGCUC CACACAAUAU AGAUGGAUAC GAAUAAGGCG UCCUUGUGG CGUCGCUGAA CCAUAGCAGG CUAGCAACGG UGCACUUGGC GGAAAGGCCU UGGGUGCUUG CUGGCGAAUU GCAAUGUCAU UUUGCGUGG GGAUAAAUCA UUUGUAUACG ACUUAGAUGU ACAACGGGGU AUUGUAAGCA GUAGAGUAGC CUUGUUGUUA C GAUCUGCU GAGAUUAAGC CUUUGUUGUC UGAUUUGU

Macromolecule #5: tRNA

• Macromolecule: Name: tRNA / type: rna / ID: 5 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 24.445512 KDa

Sequence

String:

UUCCUCGUGG CCCAAUGGUC ACGGCGUCUG GCUUCGAACC AGAAGAUUCC AGGUUCAAGU CCUGGCGGGG AAGCCA

Macromolecule #6: 40S ribosomal protein S0-A

• Macromolecule: Name: 40S ribosomal protein S0-A / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 22.811074 KDa

Sequence

String:

SLPATFDLTP EDAQLLLAAN THLGARNVQV HQEPYVFNAR PDGVHVINVG KTWEKLVLAA RIIAAIPNPE DVVAISSRTF GQRAVLKFA AHTGATPIAG RFTPGSFTNY ITRSFKEPRL VIVTDPRSDA QAIKEASYVN IPVIALTDLD SPSEFVDVAI P CNNRGKHS IGLIWYLLAR EVLRLRGALV DRTQPWSIMP DLYFYRDP

Macromolecule #7: 40S ribosomal protein S1-A

• Macromolecule: Name: 40S ribosomal protein S1-A / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 28.798467 KDa

Sequence

String:

MAVGKNKRLS KGKKGQKKRV VDPFTRKEWF DIKAPSTFEN RNVGKTLVNK STGLKSASDA LKGRVVEVCL ADLQGSEDHS FRKIKLRVD EVQGKNLLTN FHGMDFTTDK LRSMVRKWQT LIEANVTVKT SDDYVLRIFA IAFTRKQANQ VKRHSYAQSS H IRAIRKVI SEILTKEVQG STLAQLTSKL IPEVINKEIE NATKDIFPLQ NIHVRKVKLL KQPKFDVGAL MALHGEGSGE EK GKKVTGF KDEVLETV

Macromolecule #8: RPS2 isoform 1

• Macromolecule: Name: RPS2 isoform 1 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 23.08485 KDa

Sequence

String:

GWVPVTKLGR LVKAGKITTI EEIFLHSLPV KEFQIIDTLL PGLQDEVMNI KPVQKQTRAG QRTRFKAVVV VGDSNGHVGL GIKTAKEVA GAIRAGIIIA KLSVIPIRRG YWGTNLGQPH SLATKTTGKC GSVTVRLIPA PRGSGIVASP AVKKLLQLAG V EDVYTQSN GKTRTLENTL KAAFVAIGNT YGFLTPNLWA EQPLPVSPLD IYSDEASA

Macromolecule #9: RPS3 isoform 1

• Macromolecule: Name: RPS3 isoform 1 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 24.631713 KDa

Sequence

String:

LISKKRKLVA DGVFYAELNE FFTRELAEEG YSGVEVRVTP TKTEVIIRAT RTQDVLGENG RRINELTLLV QKRFKYAPGT IVLYAERVQ DRGLSAVAQA ESMKFKLLNG LAIRRAAYGV VRYVMESGAK GCEVVVSGKL RAARAKAMKF ADGFLIHSGQ P VNDFIDTA TRHVLMRQGV LGIKVKIMRD PAKSRTGPKA LPDAVTIIEP KEEEPILAPS VKDY

Macromolecule #10: 40S ribosomal protein S4-A

• Macromolecule: Name: 40S ribosomal protein S4-A / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 29.167926 KDa

Sequence

String:

ARGPKKHLKR LAAPHHWLLD KLSGCYAPRP SAGPHKLRES LPLIVFLRNR LKYALNGREV KAILMQRHVK VDGKVRTDTT YPAGFMDVI TLDATNENFR LVYDVKGRFA VHRITDEEAS YKLGKVKKVQ LGKKGVPYVV THDGRTIRYP DPNIKVNDTV K IDLASGKI TDFIKFDAGK LVYVTGGRNL GRIGTIVHKE RHDGGFDLVH IKDSLDNTFV TRLNNVFVIG EQGKPYISLP KG KGIKLSI AEERDRRRAQ Q

Macromolecule #11: Rps5p

• Macromolecule: Name: Rps5p / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 22.908338 KDa

Sequence

String:

FTPVVLATPI PEEVQQAQTE IKLFNKWSFE EVEVKDASLV DYVQVRQPIF VAHTAGRYAN KRFRKAQCPI IERLTNSLMM NGRNNGKKL KAVRIIKHTL DIINVLTDQN PIQVVVDAIT NTGPREDTTR VGGGGAARRQ AVDVSPLRRV NQAIALLTIG A REAAFRNI KTIAETLAEE LINAAKGSST SYAIKKKDEL ERVAKSNR

Macromolecule #12: 40S ribosomal protein S6-A

• Macromolecule: Name: 40S ribosomal protein S6-A / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 26.181445 KDa

Sequence

String:

MKLNISYPVN GSQKTFEIDD EHRIRVFFDK RIGQEVDGEA VGDEFKGYVF KISGGNDKQG FPMKQGVLLP TRIKLLLTKN VSCYRPRRD GERKRKSVRG AIVGPDLAVL ALVIVKKGEQ ELEGLTDTTV PKRLGPKRAN NIRKFFGLSK EDDVRDFVIR R EVTKGEKT YTKAPKIQRL VTPQRLQRKR HQRALKVRNA QAQREAAAEY AQLLAKRLSE RKAEKAEIRK

Macromolecule #13: 40S ribosomal protein S7-A

• Macromolecule: Name: 40S ribosomal protein S7-A / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 21.000492 KDa

Sequence

String:

PQAKILSQAP TELELQVAQA FVELENSSPE LKAELRPLQF KSIREIDVAG GKKALAIFVP VPSLAGFHKV QTKLTRELEK KFQDRHVIF LAERRILPKP SRTSRQVQKR PRSRTLTAVH DKILEDLVFP TEIVGKRVRY LVGGNKIQKV LLDSKDVQQI D YKLESFQA VYNKLTGKQI VFEIPS

Macromolecule #14: 40S ribosomal protein S8-B

• Macromolecule: Name: 40S ribosomal protein S8-B / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 22.537803 KDa

Sequence

String:

MGISRDSRHK RSATGAKRAQ FRKKRKFELG RQPANTKIGA KRIHSVRTRG GNKKYRALRI ETGNFSWASE GISKKTRIAG VVYHPSNNE LVRTNTLTKA AIVQIDATPF RQWFEAHYGQ TLGKKKNVKE EETVAKSKNA ERKWAARAAS AKIESSVESQ F SAGRLYAC ISSRPGQSGR CDGYILEGEE LAFYLRRLTA KK

Macromolecule #15: 40S ribosomal protein S9-A

• Macromolecule: Name: 40S ribosomal protein S9-A / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 21.081549 KDa

Sequence

String:

PRAPRTYSKT YSTPKRPYES SRLDAELKLA GEFGLKNKKE IYRISFQLSK IRRAARDLLT RDEKDPKRLF EGNALIRRLV RVGVLSEDK KKLDYVLALK VEDFLERRLQ TQVYKLGLAK SVHHARVLIT QRHIAVGKQI VNIPSFMVRL DSEKHIDFAP T SPFGGARP GRVARRNAAR KAEASG

Macromolecule #16: 40S ribosomal protein S10-A

• Macromolecule: Name: 40S ribosomal protein S10-A / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 11.042562 KDa

Sequence

String:

MLMPKEDRNK IHQYLFQEGV VVAKKDFNQA KHEEIDTKNL YVIKALQSLT SKGYVKTQFS WQYYYYTLTE EGVEYLREYL NLPEHIVPG TYI

Macromolecule #17: 40S ribosomal protein S11-A

• Macromolecule: Name: 40S ribosomal protein S11-A / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 16.46033 KDa

Sequence

String:

STELTVQSER AFQKQPHIFN NPKVKTSKRT KRWYKNAGLG FKTPKTAIEG SYIDKKCPFT GLVSIRGKIL TGTVVSTKMH RTIVIRRAY LHYIPKYNRY EKRHKNVPVH VSPAFRVQVG DIVTVGQCRP ISKTVRFNVV KVSAA

Macromolecule #18: 40S ribosomal protein S12

• Macromolecule: Name: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 13.028008 KDa

Sequence

String:

TIEDALKVVL RTALVHDGLA RGLRESTKAL TRGEALLVVL VSSVTEANII KLVEGLANDP ENKVPLIKVA DAKQLGEWAG LGKIDREGN ARKVVGASVV VVKNWGAETD ELSMIMEHFS QQ

Macromolecule #19: 40S ribosomal protein S13

• Macromolecule: Name: 40S ribosomal protein S13 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 16.928748 KDa

Sequence

String:

GRMHSAGKGI SSSAIPYSRN APAWFKLSSE SVIEQIVKYA RKGLTPSQIG VLLRDAHGVT QARVITGNKI MRILKSNGLA PEIPEDLYY LIKKAVSVRK HLERNRKDKD AKFRLILIES RIHRLARYYR TVAVLPPNWK YESATASALV N

Macromolecule #20: 40S ribosomal protein S14-B

• Macromolecule: Name: 40S ribosomal protein S14-B / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 13.446426 KDa

Sequence

String:

SQVFGVARIY ASFNDTFVHV TDLSGKETIA RVTGGMKVKA DRDESSPYAA MLAAQDVAAK CKEVGITAVH VKIRATGGTR TKTPGPGGQ AALRALARSG LRIGRIEDVT PVPSDSTRKK GGRRGRRL

Macromolecule #21: RPS15 isoform 1

• Macromolecule: Name: RPS15 isoform 1 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 13.265591 KDa

Sequence

String:

KTHSYRGVDL EKLLEMSTED FVKLAPARVR RRFARGMTSK PAGFMKKLRA AKLAAPENEK PAPVRTHMRN MIIVPEMIGS VVGIYNGKA FNQVEIRPEM LGHYLGEFSI TYTPVRHG

Macromolecule #22: 40S ribosomal protein S16-A

• Macromolecule: Name: 40S ribosomal protein S16-A / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 15.659216 KDa

Sequence

String:

AVPSVQTFGK KKSATAVAHV KAGKGLIKVN GSPITLVEPE ILRFKVYEPL LLVGLDKFSN IDIRVRVTGG GHVSQVYAIR QAIAKGLVA YHQKYVDEQS KNELKKAFTS YDRTLLIADS RRPEPKKFGG KGARSRFQKS YR

Macromolecule #23: 40S ribosomal protein S17-A

• Macromolecule: Name: 40S ribosomal protein S17-A / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 15.820413 KDa

Sequence

String:

MGRVRTKTVK RASKALIERY YPKLTLDFQT NKRLCDEIAT IQSKRLRNKI AGYTTHLMKR IQKGPVRGIS FKLQEEERER KDQYVPEVS ALDLSRSNGV LNVDNQTSDL VKSLGLKLPL SVINVSAQRD RRYRKRV

Macromolecule #24: 40S ribosomal protein S18-A

• Macromolecule: Name: 40S ribosomal protein S18-A / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 16.940443 KDa

Sequence

String:

SLVVQEQGSF QHILRLLNTN VDGNIKIVYA LTTIKGVGRR YSNLVCKKAD VDLHKRAGEL TQEELERIVQ IMQNPTHYKI PAWFLNRQN DITDGKDYHT LANNVESKLR DDLERLKKIR AHRGIRHFWG LRVRGQHTKT TGRRRA

Macromolecule #25: 40S ribosomal protein S19-A

• Macromolecule: Name: 40S ribosomal protein S19-A / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 15.81093 KDa

Sequence

String:

PGVSVRDVAA QDFINAYASF LQRQGKLEVP GYVDIVKTSS GNEMPPQDAE GWFYKRAASV ARHIYMRKQV GVGKLNKLYG GAKSRGVRP YKHIDASGSI NRKVLQALEK IGIVEISPKG GRRISENGQR DLDRIAAQTL EEDE

Macromolecule #26: RPS20 isoform 1

• Macromolecule: Name: RPS20 isoform 1 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 11.363321 KDa

Sequence

String:

IKIRITLTST KVKQLENVSS NIVKNAEQHN LVKKGPVRLP TKVLKISTRK TPNGEGSKTW ETYEMRIHKR YIDLEAPVQI VKRITQITI EPGVDVEVVV A

Macromolecule #27: 40S ribosomal protein S21-A

• Macromolecule: Name: 40S ribosomal protein S21-A / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 9.758829 KDa

Sequence

String:

MENDKGQLVE LYVPRKCSAT NRIIKADDHA SVQINVAKVD EEGRAIPGEY VTYALSGYVR SRGESDDSLN RLAQNDGLLK NVWSYSR

Macromolecule #28: RPS22A isoform 1

• Macromolecule: Name: RPS22A isoform 1 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 14.518867 KDa

Sequence

String:

TRSSVLADAL NAINNAEKTG KRQVLIRPSS KVIIKFLQVM QKHGYIGEFE YIDDHRSGKI VVQLNGRLNK CGVISPRFNV KIGDIEKWT ANLLPARQFG YVILTTSAGI MDHEEARRKH VSGKILGFVY

Macromolecule #29: 40S ribosomal protein S23-A

• Macromolecule: Name: 40S ribosomal protein S23-A / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 15.942699 KDa

Sequence

String:

GKGKPRGLNS ARKLRVHRRN NRWAENNYKK RLLGTAFKSS PFGGSSHAKG IVLEKLGIES KQPNSAIRKC VRVQLIKNGK KVTAFVPND GCLNFVDEND EVLLAGFGRK GKAKGDIPGV RFKVVKVSGV SLLALWKEKK EKPRS

Macromolecule #30: 40S ribosomal protein S24-A

• Macromolecule: Name: 40S ribosomal protein S24-A / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 15.23165 KDa

Sequence

String:

SDAVTIRTRK VISNPLLARK QFVVDVLHPN RANVSKDELR EKLAEVYKAE KDAVSVFGFR TQFGGGKSVG FGLVYNSVAE AKKFEPTYR LVRYGLAEKV EKASRQQRKQ KKNRDKKIFG TGKRLAKKVA RRNAD

Macromolecule #31: 40S ribosomal protein S25

• Macromolecule: Name: 40S ribosomal protein S25 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 9.540301 KDa

Sequence

String:

KKKWSKKSMK DRAQHAVILD QEKYDRILKE VPTYRYVSVS VLVDRLKIGG SLARIALRHL EKEGIIKPIS KHSKQAIYTR AT

Macromolecule #32: 40S ribosomal protein S26

• Macromolecule: Name: 40S ribosomal protein S26 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 11.022989 KDa

Sequence

String:

PKKRASNGRN KKGRGHVKPV RCVNCSKSIP KDKAIKRMAI RNIVEAAAVR DLSEASVYPE YALPKTYNKL HYCVSCAIHA RIVRVRSRE DRKNRAPP

Macromolecule #33: 40S ribosomal protein S27-A

• Macromolecule: Name: 40S ribosomal protein S27-A / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 8.762195 KDa

Sequence

String:

VLVQDLLHPT AASEARKHKL KTLVQGPRSY FLDVKCPGCL NITTVFSHAQ TAVTCESCST ILCTPTGGKA KLSEGTSFRR K

Macromolecule #34: RPS28A isoform 1

• Macromolecule: Name: RPS28A isoform 1 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 7.116281 KDa

Sequence

String:

TPVTLAKVIK VLGRTGSRGG VTQVRVEFLE DTSRTIVRNV KGPVRENDIL VLMESEREAR RLR

Macromolecule #35: RPS29A isoform 1

• Macromolecule: Name: RPS29A isoform 1 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 6.335303 KDa

Sequence

String:

ENVWFSHPRR YGKGSRQCRV CSSHTGLIRK YGLNICRQCF REKANDIGFN KFR

Macromolecule #36: 40S ribosomal protein S30-A

• Macromolecule: Name: 40S ribosomal protein S30-A / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 6.779086 KDa

Sequence

String:

AKVHGSLARA GKVKSQTPKV EKTEKPKKPK GRAYKRLLYT RRFVNVTLVN GKRRMNPGPS

Macromolecule #37: RPS31 isoform 1

• Macromolecule: Name: RPS31 isoform 1 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 8.388049 KDa

Sequence

String:

RKKKVYTTPK KIKHKHKKVK LAVLSYYKVD AEGKVTKLRR ECSNPTCGAG VFLANHKDRL YCGKCHSVYK VNA

Macromolecule #38: Guanine nucleotide-binding protein subunit beta-like protein

• Macromolecule: Name: Guanine nucleotide-binding protein subunit beta-like protein; type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 34.151484 KDa

Sequence

String:

EVLVLRGTLE GHNGWVTSLA TSAGQPNLLL SASRDKTLIS WKLTGDDQKF GVPVRSFKGH SHIVQDCTLT ADGAYALSAS WDKTLRLWD VATGETYQRF VGHKSDVMSV DIDKKASMII SGSRDKTIKV WTIKGQCLAT LLGHNDWVSQ VRVVPNEKAD D DSVTIISA GNDKMVKAWN LNQFQIEADF IGHNSNINTL TASPDGTLIA SAGKDGEIML WNLAAKKAMY TLSAQDEVFS LA FSPNRYW LAAATATGIK VFSLDPQYLV DDLRPEFAGY SKAAEPHAVS LAWSADGQTL FAGYTDNVIR VWQVM

Macromolecule #39: 60S ribosomal protein L2-A

• Macromolecule: Name: 60S ribosomal protein L2-A / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 27.089158 KDa

Sequence

String:

GRVIRNQRKG AGSIFTSHTR LRQGAAKLRT LDYAERHGYI RGIVKQIVHD SGRGAPLAKV VFRDPYKYRL REEIFIANEG VHTGQFIYA GKKASLNVGN VLPLGSVPEG TIVSNVEEKP GDRGALARAS GNYVIIIGHN PDENKTRVRL PSGAKKVISS D ARGVIGVI AGGGRVDKPL LKAGRAFHKY RLKRNSWPKT RGVAMNPVDH PHGGGNHQHI GKASTISRGA VSGQKAGLIA AR RTGLLRG SQKT

Macromolecule #40: 60S ribosomal protein L3

• Macromolecule: Name: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 43.719602 KDa

Sequence

String:

SHRKYEAPRH GHLGFLPRKR AASIRARVKA FPKDDRSKPV ALTSFLGYKA GMTTIVRDLD RPGSKFHKRE VVEAVTVVDT PPVVVVGVV GYVETPRGLR SLTTVWAEHL SDEVKRRFYK NWYKSKKKAF TKYSAKYAQD GAGIERELAR IKKYASVVRV L VHTQIRKT PLAQKKAHLA EIQLNGGSIS EKVDWAREHF EKTVAVDSVF EQNEMIDAIA VTKGHGFEGV THRWGTKKLP RK THRGLRK VACIGAWHPA HVMWSVARAG QRGYHSRTSI NHKIYRVGKG DDEANGATSF DRTKKTITPM GGFVHYGEIK NDF IMVKGC IPGNRKRIVT LRKSLYTNTS RKALEEVSLK WIDTASKFGK GRFQTPAEKH AFMGTLKKDL

Macromolecule #41: RPL4A isoform 1

• Macromolecule: Name: RPL4A isoform 1 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 39.027926 KDa

Sequence

String:

SRPQVTVHSL TGEATANALP LPAVFSAPIR PDIVHTVFTS VNKNKRQAYA VSEKAGHQTS AESWGTGRAV ARIPRVGGGG TGRSGQGAF GNMCRGGRMF APTKTWRKWN VKVNHNEKRY ATASAIAATA VASLVLARGH RVEKIPEIPL VVSTDLESIQ K TKEAVAAL KAVGAHSDLL KVLKSKKLRA GKGKYRNRRW TQRRGPLVVY AEDNGIVKAL RNVPGVETAN VASLNLLQLA PG AHLGRFV IWTEAAFTKL DQVWGSETVA SSKVGYTLPS HIISTSDVTR IINSSEIQSA IRPAGQATQK RTHVLKKNPL KNK QVLLRL NPYAKVFAAE KLGSKKAEKT GTKPAAVFTE TLKHD

Macromolecule #42: RPL5 isoform 1

• Macromolecule: Name: RPL5 isoform 1 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 33.415387 KDa

Sequence

String:

QKDAKSSAYS SRFQTPFRRR REGKTDYYQR KRLVTQHKAK YNTPKYRLVV RFTNKDIICQ IISSTITGDV VLAAAYSHEL PRYGITHGL TNWAAAYATG LLIARRTLQK LGLDETYKGV EEVEGEYELT EAVEDGPRPF KVFLDIGLQR TTTGARVFGA L KGASDGGL YVPHSENRFP GWDFETEEID PELLRSYIFG GHVSQYMEEL ADDDEERFSE LFKGYLADDI DADSLEDIYT SA HEAIRAD PAFKPTEKKF TKEQYAAESK KYRQTKLSKE ERAARVAAKI AALAGQQ

Macromolecule #43: 60S ribosomal protein L6-B

• Macromolecule: Name: 60S ribosomal protein L6-B / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 20.042578 KDa

Sequence

String:

MTAQQAPKWY PSEDVAAPKK TRKAVRPQKL RASLVPGTVL ILLAGRFRGK RVVYLKHLED NTLLVTGPFK VNGVPLRRVN ARYVIATST KVSVEGVNVE KFNVEYFAKE KLTKKEKKEA NLFPEQQTKE IKTERVEDQK VVDKALIAEI KKTPLLKQYL S ASFSLKNG DKPHMLKF

Macromolecule #44: 60S ribosomal protein L7-A

• Macromolecule: Name: 60S ribosomal protein L7-A / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 25.240377 KDa

Sequence

String:

AEQVAAERAA RKAANKEKRA IILERNAAYQ KEYETAERNI IQAKRDAKAA GSYYVEAQHK LVFVVRIKGI NKIPPKPRKV LQLLRLTRI NSGTFVKVTK ATLELLKLIE PYVAYGYPSY STIRQLVYKR GFGKINKQRV PLSDNAIIEA NLGKYGILSI D DLIHEIIT VGPHFKQANN FLWPFKLSNP SGGWGVPRKF KHFIQGGSFG NREEFINKLV KSMN

Macromolecule #45: 60S ribosomal protein L8

• Macromolecule: Name: 60S ribosomal protein L8 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 25.814998 KDa

Sequence

String:

NPLTHSTPKN FGIGQAVQPK RNLSRYVKWP EYVRVQRQKK ILSIRLKVPP TIAQFQYTLD RNTAAETFKL FNKYRPETAA EKKERLTKE AAAVAEGKSK QDASPKPYAV KYGLNHVVAL IENKKAKLVL IANDVDPIEL VVFLPALCKK MGVPYAIVKG K ARLGTLVN QKTSAVAALT EVRAEDEAAL AKLVSTIDAN FADKYDEVKK HWGGGILGNK AQAKMDKRAK NSDSA

Macromolecule #46: RPL9A isoform 1

• Macromolecule: Name: RPL9A isoform 1 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 21.605061 KDa

Sequence

String:

MKYIQTEQQI EVPEGVTVSI KSRIVKVVGP RGTLTKNLKH IDVTFTKVNN QLIKVAVHNG GRKHVAALRT VKSLVDNMIT GVTKGYKYK MRYVYAHFPI NVNIVEKDGA KFIEVRNFLG DKKIRNVPVR DGVTIEFSTN VKDEIVLSGN SVEDVSQNAA D LQQICRVR NKDIRKFLDG IYVSHKGFIT EDL

Macromolecule #47: RPL10 isoform 1

• Macromolecule: Name: RPL10 isoform 1 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 25.080062 KDa

Sequence

String:

ARRPARCYRY QKNKPYPKSR YNRAVPDSKI RIYDLGKKKA TVDEFPLCVH LVSNELEQLS SEALEAARIC ANKYMTTVSG RDAFHLRVR VHPFHVLRIN KMLSCAGADR LQQGMRGAWG KPHGLAARVD IGQIIFSVRT KDSNKDVVVE GLRRARYKFP G QQKIILSK KWGFTNLDRP EYLKKREAGE VKDDGAFVKF LSKKGSLENN IREFPEYFAA

Macromolecule #48: RPL11B isoform 1

• Macromolecule: Name: RPL11B isoform 1 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 19.266082 KDa

Sequence

String:

QNPMRDLKIE KLVLNISVGE SGDRLTRASK VLEQLSGQTP VQSKARYTVR TFGIRRNEKI AVHVTVRGPK AEEILERGLK VKEYQLRDR NFSATGNFGF GIDEHIDLGI KYDPSIGIFG MDFYVVMNRP GARVTRRKRC KGTVGNSHKT TKEDTVSWFK Q KYDADVLD K

Macromolecule #49: 60S ribosomal protein L13-A

• Macromolecule: Name: 60S ribosomal protein L13-A / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 21.885234 KDa

Sequence

String:

AISKNLPILK NHFRKHWQER VKVHFDQAGK KVSRRNARAT RAAKIAPRPL DLLRPVVRAP TVKYNRKVRA GRGFTLAEVK AAGLTAAYA RTIGIAVDHR RQNRNQEIFD ANVQRLKEYQ SKIIVFPRNG KAPEAEQVLS AAATFPIAQP ATDVEARAVQ D NGESAFRT LRLARSEKKF RGIREKRARE KAEAE

Macromolecule #50: 60S ribosomal protein L14-A

• Macromolecule: Name: 60S ribosomal protein L14-A / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 14.976794 KDa

Sequence

String:

TDSIVKASNW RLVEVGRVVL IKKGQSAGKL AAIVEIIDQK KVLIDGPKAG VPRQAINLGQ VVLTPLTFAL PRGARTATVS KKWAAAAVC EKWAASSWAK KIAQRERRAA LTDFERFQVM VLRKQKRYTV KKALAKA

Macromolecule #51: Ribosomal protein L15

• Macromolecule: Name: Ribosomal protein L15 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 24.351162 KDa

Sequence

String:

GAYKYLEELQ RKKQSDVLRF LQRVRVWEYR QKNVIHRAAR PTRPDKARRL GYKAKQGFVI YRVRVRRGNR KRPVPKGATY GKPTNQGVN ELKYQRSLRA TAEERVGRRA ANLRVLNSYW VNQDSTYKYF EVILVDPQHK AIRRDARYNW ICDPVHKHRE A RGLTATGK KSRGINKGHK FNNTKAGRRK TWKRQNTLSL WRYRK

Macromolecule #52: 60S ribosomal protein L16-A

• Macromolecule: Name: 60S ribosomal protein L16-A / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 22.028951 KDa

Sequence

String:

VEPVVVIDGK GHLVGRLASV VAKQLLNGQK IVVVRAEELN ISGEFFRNKL KYHDFLRKAT AFNKTRGPFH FRAPSRIFYK ALRGMVSHK TARGKAALER LKVFEGIPPP YDKKKRVVVP QALRVLRLKP GRKYTTLGKL STSVGWKYED VVAKLEAKRK V SSAEYYAK KRAFTKKVAS ANATAAESDV AKQLAALGY

Macromolecule #53: 60S ribosomal protein L17-A

• Macromolecule: Name: 60S ribosomal protein L17-A / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 20.458322 KDa

Sequence

String:

ARYGATSTNP AKSASARGSY LRVSFKNTRE TAQAINGWEL TKAQKYLEQV LDHQRAIPFR RFNSSIGRTA QGKEFGVTKA RWPAKSVKF VQGLLQNAAA NAEAKGLDAT KLYVSHIQVN QAPKQRRRTY RAHGRINKYE SSPSHIELVV TEKEEAVAKA A EKKVVRLT SRQRGRIAAQ KRIAA

Macromolecule #54: 60S ribosomal protein L18-A

• Macromolecule: Name: 60S ribosomal protein L18-A / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 20.478059 KDa

Sequence

String:

GIDHTSKQHK RSGHRTAPKS DNVYLKLLVK LYTFLARRTD APFNKVVLKA LFLSKINRPP VSVSRIARAL KQEGAANKTV VVVGTVTDD ARIFEFPKTT VAALRFTAGA RAKIVKAGGE CITLDQLAVR APKGQNTLIL RGPRNSREAV RHFGMGPHKG K APRILSTG RKFERARGRR RSKGFKV

Macromolecule #55: 60S ribosomal protein L19-A

• Macromolecule: Name: 60S ribosomal protein L19-A / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 21.631121 KDa

Sequence

String:

ANLRTQKRLA ASVVGVGKRK VWLDPNETSE IAQANSRNAI RKLVKNGTIV KKAVTVHSKS RTRAHAQSKR EGRHSGYGKR KGTREARLP SQVVWIRRLR VLRRLLAKYR DAGKIDKHLY HVLYKESKGN AFKHKRALVE HIIQAKADAQ REKALNEEAE A RRLKNRAA RDRRAQRVAE KRDALLKEDA

Macromolecule #56: 60S ribosomal protein L20-A

• Macromolecule: Name: 60S ribosomal protein L20-A / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 20.347654 KDa

Sequence

String:

AHFKEYQVIG RRLPTESVPE PKLFRMRIFA SNEVIAKSRY WYFLQKLHKV KKASGEIVSI NQINEAHPTK VKNFGVWVRY DSRSGTHNM YKEIRDVSRV AAVETLYQDM AARHRARFRS IHILKVAEIE KTADVKRQYV KQFLTKDLKF PLPHRVQKST K TFSYKRPS TFY

Macromolecule #57: 60S ribosomal protein L21-A

• Macromolecule: Name: 60S ribosomal protein L21-A / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 18.148066 KDa

Sequence

String:

GKSHGYRSRT RYMFQRDFRK HGAVHLSTYL KVYKVGDIVD IKANGSIQKG MPHKFYQGKT GVVYNVTKSS VGVIINKMVG NRYLEKRLN LRVEHIKHSK CRQEFLERVK ANAAKRAEAK AQGVAVQLKR QPAQPRESRI VSTEGNVPQT LAPVPYETFI

Macromolecule #58: 60S ribosomal protein L22-A

• Macromolecule: Name: 60S ribosomal protein L22-A / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 11.263863 KDa

Sequence

String:

QKIAKTFTVD VSSPTENGVF DPASYAKYLI DHIKVEGAVG NLGNAVTVTE DGTVVTVVST AKFSGKYLKY LTKKYLKKNQ LRDWIRFVS TKTNEYRLAF Y

Macromolecule #59: 60S ribosomal protein L23-A

• Macromolecule: Name: 60S ribosomal protein L23-A / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 14.362755 KDa

Sequence

String:

SGNGAQGTKF RISLGLPVGA IMNCADNSGA RNLYIIAVKG SGSRLNRLPA ASLGDMVMAT VKKGKPELRK KVMPAIVVRQ AKSWRRRDG VFLYFEDNAG VIANPKGEMK GSAITGPVGK ECADLWPRVA SNSGVVV

Macromolecule #60: RPL24A isoform 1

• Macromolecule: Name: RPL24A isoform 1 / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 14.617215 KDa

Sequence

String:

MKVEIDSFSG AKIYPGRGTL FVRGDSKIFR FQNSKSASLF KQRKNPRRIA WTVLFRKHHK KGITEEVAKK RSRKTVKAQR PITGASLDL IKERRSLKPE VRKAQREEKQ KADKEKKKAA KAARKAE

Macromolecule #61: 60S ribosomal protein L25

• Macromolecule: Name: 60S ribosomal protein L25 / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 13.771176 KDa

Sequence

String:

KALKVRTSAT FRLPKTLKLA RAPKYASKAV PHYNRLDSYK VIEQPITSET AMKKVEDGNI LVFQVSMKAN KYQIKKAVKE LYEVDVLKV NTLVRPNGTK KAYVRLTADY DALDIANRIG YI

Macromolecule #62: 60S ribosomal protein L26-A

• Macromolecule: Name: 60S ribosomal protein L26-A / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 14.005475 KDa

Sequence

String:

AKQSLDVSSD RRKARKAYFT APSSQRRVLL SAPLSKELRA QYGIKALPIR RDDEVLVVRG SKKGQEGKIS SVYRLKFAVQ VDKVTKEKV NGASVPINLH PSKLVITKLH LDKDRKALIQ RKGGKL

Macromolecule #63: 60S ribosomal protein L27-A

• Macromolecule: Name: 60S ribosomal protein L27-A / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 15.437166 KDa

Sequence

String:

AKFLKAGKVA VVVRGRYAGK KVVIVKPHDE GSKSHPFGHA LVAGIERYPL KVTKKHGAKK VAKRTKIKPF IKVVNYNHLL PTRYTLDVE AFKSVVSTET FEQPSQREEA KKVVKKAFEE RHQAGKNQWF FSKLRF

Macromolecule #64: 60S ribosomal protein L28

• Macromolecule: Name: 60S ribosomal protein L28 / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 16.630471 KDa

Sequence

String:

PSRFTKTRKH RGHVSAGKGR IGKHRKHPGG RGMAGGQHHH RINMDKYHPG YFGKVGMRYF HKQQAHFWKP VLNLDKLWTL IPEDKRDQY LKSASKETAP VIDTLAAGYG KILGKGRIPN VPVIVKARFV SKLAEEKIRA AGGVVELIA

Macromolecule #65: 60S ribosomal protein L29

• Macromolecule: Name: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 6.560688 KDa

Sequence

String:

AKSKNHTAHN QTRKAHRNGI KKPKTYKYPS LKGVDPKFRR NHKHALHGTA KALAAAKK

Macromolecule #66: 60S ribosomal protein L30

• Macromolecule: Name: 60S ribosomal protein L30 / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 10.487266 KDa

Sequence

String:

SINQKLALVI KSGKYTLGYK STVKSLRQGK SKLIIIAANT PVLRKSELEY YAMLSKTKVY YFQGGNNELG TAVGKLFRVG VVSILEAGD SDILTTL

Macromolecule #67: 60S ribosomal protein L31-A

• Macromolecule: Name: 60S ribosomal protein L31-A / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 12.649754 KDa

Sequence

String:

LKDVVTREYT INLHKRLHGV SFKKRAPRAV KEIKKFAKLH MGTDDVRLAP ELNQAIWKRG VKGVEYRLRL RISRKRNEEE DAKNPLFSY VEPVLVASAK GLQTVVVEED

Macromolecule #68: RPL32 isoform 1

• Macromolecule: Name: RPL32 isoform 1 / type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 14.478054 KDa

Sequence

String:

ASLPHPKIVK KHTKKFKRHH SDRYHRVAEN WRKQKGIDSV VRRRFRGNIS QPKIGYGSNK KTKFLSPSGH KTFLVANVKD LETLTMHTK TYAAEIAHNI SAKNRVVILA RAKALGIKVT NPKGRLAL

Macromolecule #69: 60S ribosomal protein L33-A

• Macromolecule: Name: 60S ribosomal protein L33-A / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 12.045935 KDa

Sequence

String:

AESHRLYVKG KHLSYQRSKR VNNPNVSLIK IEGVATPQDA QFYLGKRIAY VYRASKEVRG SKIRVMWGKV TRTHGNSGVV RATFRNNLP AKTFGASVRI FLYPSNI

Macromolecule #70: 60S ribosomal protein L34-A

• Macromolecule: Name: 60S ribosomal protein L34-A / type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 12.608831 KDa

Sequence

String:

AQRVTFRRRN PYNTRSNKIK VVKTPGGILR AQHVKKLATR PKCGDCGSAL QGISTLRPRQ YATVSKTHKT VSRAYGGSRC ANCVKERII RAFLIEEQKI VKKVVKEQTE AAK

Macromolecule #71: 60S ribosomal protein L35-A

• Macromolecule: Name: 60S ribosomal protein L35-A / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 13.811444 KDa

Sequence

String:

AGVKAYELRT KSKEQLASQL VDLKKELAEL KVQKLSRPSL PKIKTVRKSI ACVLTVINEQ QREAVRQLYK GKKYQPKDLR AKKTRALRR ALTKFEASQV TEKQRKKQIA FPQRKYAIKA

Macromolecule #72: 60S ribosomal protein L36-A

• Macromolecule: Name: 60S ribosomal protein L36-A / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 11.020063 KDa

Sequence

String:

TVKTGIAIGL NKGKKVTSMT PAPKISYKKG AASNRTKFVR SLVREIAGLS PYERRLIDLI RNSGEKRARK VAKKRLGSFT RAKAKVEEM NNIIAASRRH

Macromolecule #73: 60S ribosomal protein L37-A

• Macromolecule: Name: 60S ribosomal protein L37-A / type: protein_or_peptide / ID: 73 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 9.588045 KDa

Sequence

String:

GKGTPSFGKR HNKSHTLCNR CGRRSFHVQK KTCSSCGYPA AKTRSYNWGA KAKRRHTTGT GRMRYLKHVS RRFKNGFQTG SASKA

Macromolecule #74: RPL38 isoform 1

• Macromolecule: Name: RPL38 isoform 1 / type: protein_or_peptide / ID: 74 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 8.714363 KDa

Sequence

String:

AREITDIKQF LELTRRADVK TATVKINKKL NKAGKPFRQT KFKVRGSSSL YTLVINDAGK AKKLIQSLPP TLKVNRL

Macromolecule #75: 60S ribosomal protein L39

• Macromolecule: Name: 60S ribosomal protein L39 / type: protein_or_peptide / ID: 75 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 6.227444 KDa

Sequence

String:

AAQKSFRIKQ KMAKAKKQNR PLPQWIRLRT NNTIRYNAKR RNWRRTKMNI

Macromolecule #76: 60S ribosomal protein L40-A

• Macromolecule: Name: 60S ribosomal protein L40-A / type: protein_or_peptide / ID: 76 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 6.032321 KDa

Sequence

String:

IIEPSLKALA SKYNCDKSVC RKCYARLPPR ATNCRKRKCG HTNQLRPKKK LK

Macromolecule #77: 60S ribosomal protein L41

• Macromolecule: Name: 60S ribosomal protein L41 / type: protein_or_peptide / ID: 77 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 3.354243 KDa

Sequence

String:

MRAKWRKKRT RRLKRKRRKV RARSK

Macromolecule #78: 60S ribosomal protein L42-A

• Macromolecule: Name: 60S ribosomal protein L42-A / type: protein_or_peptide / ID: 78 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 11.840159 KDa

Sequence

String:

VNVPKTRKTY CKGKTCRKHT QHKVTQYKAG KASLFAQGKR RYDRKQSGFG GQTKPVFHKK AKTTKKVVLR LECVKCKTRA QLTLKRCKH FELGGEKKQK GQAL

Macromolecule #79: 60S ribosomal protein L43-A

• Macromolecule: Name: 60S ribosomal protein L43-A / type: protein_or_peptide / ID: 79 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 9.981756 KDa

Sequence

String:

AKRTKKVGIT GKYGVRYGSS LRRQVKKLEI QQHARYDCSF CGKKTVKRGA AGIWTCSCCK KTVAGGAYTV STAAAATVRS TIRRLREMV EA

Macromolecule #80: RQC trigger complex helicase SLH1

• Macromolecule: Name: RQC trigger complex helicase SLH1 / type: protein_or_peptide / ID: 80 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 225.127078 KDa
• Recombinant expression: Organism: Saccharomyces cerevisiae (brewer's yeast)

Sequence

String:

MSTEYSADSS KSFMIAMQSM IDTSQTFNLD RSKISLPDFD DELKKVQKDE QNQRTELTVL SQDRNDWDDI FEEFKDISFA QLQSIIDSY KTKNAVAVYK KIGKLINEAE TTLSSNVLLE TVLQMVYKHQ KQELEKELLD FLGTGNIDLV SLLLQHRRMI V ATPIETTI LLIKNAVNST PEFLTQQDIR NQVLKSAEDA KNRKLNPATK IIKYPHVFRK YEAGSTTAMA FAGQKFTLPV GT TRMSYNT HEEIIIPAAD QASNKNYLYT KLLKISDLDH FCKTVFPYET LNQIQSLVYP VAYKTNENML ICAPTGAGKT DIA LLTIIN TIKQFSVVNG ENEIDIQYDD FKVIYVAPLK ALAAEIVDKF SKKLAPFNIQ VRELTGDMQL TKAEILATQV IVTT PEKWD VVTRKANGDN DLVSKVKLLI IDEVHLLHED RGSVIETLVA RTLRQVESSQ SMIRIIGLSA TLPNFMDVAD FLGVN RQIG MFYFDQSFRP KPLEQQLLGC RGKAGSRQSK ENIDKVAYDK LSEMIQRGYQ VMVFVHSRKE TVKSARNFIK LAESNH EVD LFAPDPIEKD KYSRSLVKNR DKDMKEIFQF GFGIHHAGMA RSDRNLTEKM FKDGAIKVLC CTATLAWGVN LPADCVI IK GTQVYDSKKG GFIDLGISDV IQIFGRGGRP GFGSANGTGI LCTSNDRLDH YVSLITQQHP IESRFGSKLV DNLNAEIS L GSVTNVDEAI EWLGYTYMFV RMRKNPFTYG IDWEEIANDP QLYERRRKMI VVAARRLHAL QMIVFDEVSM HFIAKDLGR VSSDFYLLNE SVEIFNQMCD PRATEADVLS MISMSSEFDG IKFREEESKE LKRLSDESVE CQIGSQLDTP QGKANVLLQA YISQTRIFD SALSSDSNYV AQNSVRICRA LFLIGVNRRW GKFSNVMLNI CKSIEKRLWA FDHPLCQFDL PENIIRRIRD T KPSMEHLL ELEADELGEL VHNKKAGSRL YKILSRFPKI NIEAEIFPIT TNVMRIHIAL GPDFVWDSRI HGDAQFFWVF VE ESDKSQI LHFEKFILNR RQLNNQHEMD FMIPLSDPLP PQVVVKVVSD TWIGCESTHA ISFQHLIRPF NETLQTKLLK LRP LPTSAL QNPLIESIYP FKYFNPMQTM TFYTLYNTNE NAFVGSPTGS GKTIVAELAI WHAFKTFPGK KIVYIAPMKA LVRE RVDDW RKKITPVTGD KVVELTGDSL PDPKDVHDAT IVITTPEKFD GISRNWQTRK FVQDVSLIIM DEIHLLASDR GPILE MIVS RMNYISSQTK QPVRLLGMST AVSNAYDMAG WLGVKDHGLY NFPSSVRPVP LKMYIDGFPD NLAFCPLMKT MNKPVF MAI KQHSPDKPAL IFVASRRQTR LTALDLIHLC GMEDNPRRFL NIDDEEELQY YLSQVTDDTL KLSLQFGIGL HHAGLVQ KD RSISHQLFQK NKIQILIATS TLAWGVNLPA HLVIIKGTQF FDAKIEGYRD MDLTDILQMM GRAGRPAYDT TGTAIVYT K ESKKMFYKHF LNVGFPVESS LHKVLDDHLG AEITSGSITN KQEALDFLSW TFLFRRAHHN PTYYGIEDDT STAGVSEHL SSLIDSTLEN LRESQCVLLH GDDIVATPFL SISSYYYISH LTIRQLLKQI HDHATFQEVL RWLSLAVEYN ELPVRGGEII MNEEMSQQS RYSVESTFTD EFELPMWDPH VKTFLLLQAH LSRVDLPIAD YIQDTVSVLD QSLRILQAYI DVASELGYFH T VLTMIKMM QCIKQGYWYE DDPVSVLPGL QLRRIKDYTF SEQGFIEMTP QQKKKKLLTL EEIGRFGYKK LLNVFDQLTF GM TESEDTK KRFVSVCQRL PVLEGMKFEE QENNEVLTFY SKHLSSKHNN GFEVYCDKFP KIQKELWFLI GHKGDELLMI KRC QPKQMN KEVIIHCDLF IPEEIRGEEL QFSLINDALG LRYDMVHKLI S

Macromolecule #81: CUE3 isoform 1

• Macromolecule: Name: CUE3 isoform 1 / type: protein_or_peptide / ID: 81 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 34.241949 KDa
• Recombinant expression: Organism: Saccharomyces cerevisiae (brewer's yeast)

Sequence

String:

MLSRYNRVIE INGGNADISL PIVKFPPFKL RAQLIEKDPV VWLHLIETYV TYFEYLMQGA NVELLDESTL DHLRLFLRTY LHEIADEEG KLLSLGINHD VSEQLYLLKG WIFSLIKKCG LLHLQIFGDS LWNLIKVYVR RNPDSIRGLI DGSLKPRINT Q RVQLDKSY QVQQHLKQLI ESGKFKRIDL RCVEDLLSAK SMQPNKFAEN FFTANWIEIL EALWAKGQGR GHKEARELII IS LFSVSAD RLLKITKELG ISNFETLALY PLLGTMLINE GVHKRLPDLK SKLLFLNLGG

Macromolecule #82: RQC trigger complex subunit RQT4

• Macromolecule: Name: RQC trigger complex subunit RQT4 / type: protein_or_peptide / ID: 82 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 60.903078 KDa
• Recombinant expression: Organism: Saccharomyces cerevisiae (brewer's yeast)

Sequence

String:

MTRKQAIDYA IKQVPQILPL EESDVKALCE QVLSTSSDDP EQIASKFLEF LGHEDLSFEF VMKFNELLNQ NDKKEEKKTK NVHLEHTAP TSWKNESKQP TNNYINKKGD EKPKKLKDEK KSSTTRPTVQ PSNQSTQSNP IKEKKEHRSK GKLQSLQEID E AIKMLELR DSGSSKNCNC QGTRHPVFDI APNCLHCGKV VCVIEGLNKG KCGHCHEQLI SDNERTQMVE ILNQEKNELN GS SSSLSNA SNGANVPKKK TKTYKITSGM GKNLFAEQDK LFDFIERKRE RERKRNEVLK LQEEKEESEA KERQASEHDH KAE ENPELL AAQERLDRLL YFQDTSAERT KIIDNASDFD MNQEVGLWGS ARERALALKK QQRNLRKWEK VEKERNGRRE KYVV SMNIG SNGKVTMTEV PKDTENVIAG SDDDISDISD EEDISDLKHI HALKSEINTT KSLENLHLQS KAWDYERDKK QFDRP TYVK KNSDTAQQNR KTEEKAHDMQ AYDLKSRVQV DQNADASVEQ NILAVL

Macromolecule #83: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 83 / Number of copies: 86 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #84: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 84 / Number of copies: 9 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.5
• Grid: Model: Quantifoil R3/3 / Material: COPPER / Pretreatment - Type: GLOW DISCHARGE
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 44.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 0.5 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 4.3 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 17885
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD