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TitleStructural basis for assembly of vertical single β-barrel viruses.
Journal, issue, pagesNat Commun, Vol. 10, Issue 1, Page 1184, Year 2019
Publish dateMar 12, 2019
AuthorsIsaac Santos-Pérez / Diego Charro / David Gil-Carton / Mikel Azkargorta / Felix Elortza / Dennis H Bamford / Hanna M Oksanen / Nicola G A Abrescia /
PubMed AbstractThe vertical double β-barrel major capsid protein (MCP) fold, fingerprint of the PRD1-adeno viral lineage, is widespread in many viruses infecting organisms across the three domains of life. The ...The vertical double β-barrel major capsid protein (MCP) fold, fingerprint of the PRD1-adeno viral lineage, is widespread in many viruses infecting organisms across the three domains of life. The discovery of PRD1-like viruses with two MCPs challenged the known assembly principles. Here, we present the cryo-electron microscopy (cryo-EM) structures of the archaeal, halophilic, internal membrane-containing Haloarcula californiae icosahedral virus 1 (HCIV-1) and Haloarcula hispanica icosahedral virus 2 (HHIV-2) at 3.7 and 3.8 Å resolution, respectively. Our structures reveal proteins located beneath the morphologically distinct two- and three-tower capsomers and homopentameric membrane proteins at the vertices that orchestrate the positioning of pre-formed vertical single β-barrel MCP heterodimers. The cryo-EM based structures together with the proteomics data provide insights into the assembly mechanism of this type of viruses and into those with membrane-less double β-barrel MCPs.
External linksNat Commun / PubMed:30862777 / PubMed Central
MethodsEM (single particle)
Resolution3.74 - 18.2 Å
Structure data

EMDB-0050:
DNA-devoid HCIV-1 virus particle
Method: EM (single particle) / Resolution: 14.9 Å

EMDB-0072:
Localized reconstructed spike HCIV-1
Method: EM (single particle) / Resolution: 18.2 Å

EMDB-0073:
Localized reconstructed spike HCIV-1
Method: EM (single particle) / Resolution: 8.9 Å

EMDB-0131:
HHIV-2 pentameric vertex complex
Method: EM (single particle) / Resolution: 7.7 Å

0172

6h82

EMDB-0172, PDB-6h82:
Cryo-EM structure of the archaeal extremophilic internal membrane containing Haloarcula hispanica icosahedral virus 2 (HHIV-2) at 3.78 Angstroms resolution.
Method: EM (single particle) / Resolution: 3.78 Å

0174

6h9c

EMDB-0174, PDB-6h9c:
Cryo-EM structure of archaeal extremophilic internal membrane-containing Haloarcula californiae icosahedral virus 1 (HCIV-1) at 3.74 Angstroms resolution.
Method: EM (single particle) / Resolution: 3.74 Å

Source
  • haloarcula hispanica icosahedral virus 2
  • haloarcula californiae atcc 33799 (Halophile)
KeywordsVIRUS / single vertical beta-barrel virus / archaeal / membrane-containing / quasi-atomic resolution / vertical single beta-barrel virus / internal membrane-containing archaeal virus.

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