EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Human coronavirus HKU1 spike structures reveal the basis for sialoglycan specificity and carbohydrate-promoted conformational changes.
ジャーナル・号・ページ	Nat Commun, Vol. 16, Issue 1, Page 4158, Year 2025
掲載日	2025年5月5日
著者	Min Jin / Zaky Hassan / Zhijie Li / Ying Liu / Aleksandra Marakhovskaia / Alan H M Wong / Adam Forman / Mark Nitz / Michel Gilbert / Hai Yu / Xi Chen / James M Rini /
PubMed 要旨	The human coronavirus HKU1 uses both sialoglycoconjugates and the protein transmembrane serine protease 2 (TMPRSS2) as receptors. Carbohydrate binding leads to the spike protein up conformation ...The human coronavirus HKU1 uses both sialoglycoconjugates and the protein transmembrane serine protease 2 (TMPRSS2) as receptors. Carbohydrate binding leads to the spike protein up conformation required for TMPRSS2 binding, an outcome suggesting a distinct mechanism for driving fusion of the viral and host cell membranes. Nevertheless, the conformational changes promoted by carbohydrate binding have not been fully elucidated and the basis for HKU1's carbohydrate binding specificity remains unknown. Reported here are high resolution cryo-EM structures of the HKU1 spike protein trimer in its apo form and in complex with the carbohydrate moiety of a candidate carbohydrate receptor, the 9-O-acetylated GD3 ganglioside. The structures show that the spike monomer can exist in four discrete conformational states and that progression through them would promote the up conformation upon carbohydrate binding. We also show that a six-amino-acid insert is a determinant of HKU1's specificity for gangliosides containing a 9-O-acetylated α2-8-linked disialic acid moiety and that HKU1 shows weak affinity for the 9-O-acetylated sialic acids found on decoy receptors such as mucins.
リンク	Nat Commun / PubMed:40324974 / PubMed Central
手法	EM (単粒子)
解像度	1.93 - 3.34 Å
構造データ	44874 9bsw EMDB-44874, PDB-9bsw: Cryo-EM structure of HCoV-HKU1 Spike glycoprotein (DDA state) 手法: EM (単粒子) / 解像度: 2.06 Å 44875 9bsx EMDB-44875, PDB-9bsx: Cryo-EM structure of HCoV-HKU1 glycoprotein in complex with 9O-acetyl GD3 sialoglycan (DAA state) 手法: EM (単粒子) / 解像度: 2.37 Å 44876 9bsy EMDB-44876, PDB-9bsy: Cryo-EM structure of HCoV-HKU1 glycoprotein in complex with 9O-acetyl GD3 sialoglycan (DAU state) 手法: EM (単粒子) / 解像度: 2.58 Å 44877 9bsz EMDB-44877, PDB-9bsz: Cryo-EM structure of HCoV-HKU1 glycoprotein in complex with 9O-acetyl GD3 sialoglycan (AUU state) 手法: EM (単粒子) / 解像度: 2.49 Å 44878 9bt0 EMDB-44878, PDB-9bt0: Cryo-EM structure of HCoV-HKU1 Spike glycoprotein (DDD state) 手法: EM (単粒子) / 解像度: 2.23 Å 44879 9bt1 EMDB-44879, PDB-9bt1: Cryo-EM structure of HCoV-HKU1 glycoprotein in complex with 9O-acetyl GD3 sialoglycan (UUU state) 手法: EM (単粒子) / 解像度: 2.22 Å 44880 9bt2 EMDB-44880, PDB-9bt2: Cryo-EM structure of HCoV-HKU1 glycoprotein D1 (Down State, locally refined) 手法: EM (単粒子) / 解像度: 1.93 Å 44884 9bt9 EMDB-44884, PDB-9bt9: Cryo-EM Structure of HKU1 spike D1 Domain (Active state, locally refined) 手法: EM (単粒子) / 解像度: 2.11 Å 44885 9bta EMDB-44885, PDB-9bta: Cryo-EM density map of HKU1 spike glycoprotein D1 domain in complex with 9O-acetyl GD3 sialoglycan (Down_alt state, locally refined) 手法: EM (単粒子) / 解像度: 2.39 Å 44886 9btb EMDB-44886, PDB-9btb: Cryo-EM density map of HKU1 spike glycoprotein D1 domain in complex with 9O-acetyl GD3 sialoglycan (Active state, locally refined) 手法: EM (単粒子) / 解像度: 2.33 Å 44887 9btc EMDB-44887, PDB-9btc: Cryo-EM density map of HKU1 spike glycoprotein D1 domain in complex with 9O-acetyl GD3 sialoglycan (Up state, locally refined) 手法: EM (単粒子) / 解像度: 2.41 Å 44888 9btd EMDB-44888, PDB-9btd: Cryo-EM structure of HCoV-HKU1 glycoprotein in complex with 9O-acetyl GD3 sialoglycan (DDA state) 手法: EM (単粒子) / 解像度: 2.43 Å 48803 9n12 EMDB-48803, PDB-9n12: Cryo-EM structure of HCoV-HKU1 glycoprotein (mutant T31VPR34 to GGGG) 手法: EM (単粒子) / 解像度: 3.18 Å 48804 9n13 EMDB-48804, PDB-9n13: Cryo-EM structure of HCoV-HKU1 glycoprotein D1 Domain (mutant T31VPR34 to GGGG) 手法: EM (単粒子) / 解像度: 3.34 Å 48805 9n14 EMDB-48805, PDB-9n14: Cryo-EM structure of HCoV-HKU1 glycoprotein in complex with 9OAc-GD3(mutant T31VPR34 to GGGG) 手法: EM (単粒子) / 解像度: 3.08 Å 48806 9n15 EMDB-48806, PDB-9n15: Cryo-EM structure of HCoV-HKU1 glycoprotein D1 Domain in complex with 9OAc-GD3(mutant T31VPR34 to GGGG) 手法: EM (単粒子) / 解像度: 3.2 Å 48807 9n16 EMDB-48807, PDB-9n16: Cryo-EM structure of HCoV-HKU1 glycoprotein (Deletion 33Pro34Arg) 手法: EM (単粒子) / 解像度: 3.01 Å 48808 9n17 EMDB-48808, PDB-9n17: Cryo-EM structure of HCoV-HKU1 glycoprotein D1 Domain (Deletion 33Pro34Arg) 手法: EM (単粒子) / 解像度: 3.09 Å 48809 9n18 EMDB-48809, PDB-9n18: Cryo-EM structure of HCoV-HKU1 spike glycoprotein in complex with 9OAc-GD3 tetrasacchride (Deletion 33Pro34Arg) 手法: EM (単粒子) / 解像度: 3.02 Å 48810 9n19 EMDB-48810, PDB-9n19: Cryo-EM structure of HCoV-HKU1spike glycoprotein D1 Domain in complex with 9OAc-GD3 tetrasacchride (Deletion 33Pro34Arg) 手法: EM (単粒子) / 解像度: 3.02 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン PDB-1ar1: Structure at 2.7 Angstrom Resolution of the Paracoccus Denitrificans two-subunit Cytochrome C Oxidase Complexed with an Antibody Fv Fragment PDB-1ar2: DISULFIDE-FREE IMMUNOGLOBULIN FRAGMENT
由来	human coronavirus hku1 (ウイルス)
キーワード	VIRAL PROTEIN / HCoV-HKU1 spike glycoprotein ectodomain / proline stablized / HKU1 spike glycoprotein ectodomain