EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Mechanisms of actin filament severing and elongation by formins.
ジャーナル・号・ページ	Nature, Vol. 632, Issue 8024, Page 437-442, Year 2024
掲載日	2024年6月6日
著者	Nicholas J Palmer / Kyle R Barrie / Roberto Dominguez /
PubMed 要旨	Humans express 15 formins that play crucial roles in actin-based processes, including cytokinesis, cell motility and mechanotransduction. However, the lack of structures bound to the actin filament ...Humans express 15 formins that play crucial roles in actin-based processes, including cytokinesis, cell motility and mechanotransduction. However, the lack of structures bound to the actin filament (F-actin) has been a major impediment to understanding formin function. Whereas formins are known for their ability to nucleate and elongate F-actin, some formins can additionally depolymerize, sever or bundle F-actin. Two mammalian formins, inverted formin 2 (INF2) and diaphanous 1 (DIA1, encoded by DIAPH1), exemplify this diversity. INF2 shows potent severing activity but elongates weakly whereas DIA1 has potent elongation activity but does not sever. Using cryo-electron microscopy (cryo-EM) we show five structural states of INF2 and two of DIA1 bound to the middle and barbed end of F-actin. INF2 and DIA1 bind differently to these sites, consistent with their distinct activities. The formin-homology 2 and Wiskott-Aldrich syndrome protein-homology 2 (FH2 and WH2, respectively) domains of INF2 are positioned to sever F-actin, whereas DIA1 appears unsuited for severing. These structures also show how profilin-actin is delivered to the fast-growing barbed end, and how this is followed by a transition of the incoming monomer into the F-actin conformation and the release of profilin. Combined, the seven structures presented here provide step-by-step visualization of the mechanisms of F-actin severing and elongation by formins.
リンク	Nature / PubMed:38843827 / PubMed Central
手法	EM (単粒子)
解像度	2.86 - 4.38 Å
構造データ	EMDB-44009: INF2 at the Barbed End of F-actin, Consensus refinement 手法: EM (単粒子) / 解像度: 3.37 Å EMDB-44010: INF2 at the Barbed End of F-Actin, Leading WH2 focus refinement 手法: EM (単粒子) / 解像度: 4.17 Å EMDB-44011: INF2 at the Barbed End of F-Actin, Trailing WH2 focus refinement 手法: EM (単粒子) / 解像度: 4.09 Å 44012 9az4 EMDB-44012, PDB-9az4: INF2 at the Barbed End of F-Actin 手法: EM (単粒子) / 解像度: 3.37 Å 44018 9azp EMDB-44018, PDB-9azp: INF2 at the Barbed End of F-Actin with Incoming Profilin-Actin 手法: EM (単粒子) / 解像度: 3.79 Å 44019 9azq EMDB-44019, PDB-9azq: INF2 at the Barbed End of F-Actin with Incoming Actin 手法: EM (単粒子) / 解像度: 3.82 Å EMDB-44020: INF2 in the Middle of F-Actin, Up state consensus refinement 手法: EM (単粒子) / 解像度: 2.96 Å EMDB-44022: INF2 in the Middle of F-actin, Up state focus refinement for the static WH2 手法: EM (単粒子) / 解像度: 3.18 Å EMDB-44023: INF2 in the Middle of Actin filament 手法: EM (単粒子) / 解像度: 2.86 Å EMDB-44024: INF2 at the Middle of F-Actin, Up state mobile FH2 local refinement 手法: EM (単粒子) / 解像度: 3.03 Å EMDB-44025: INF2 in the Middle of Actin Filament, Up state local refinement for static FH2 手法: EM (単粒子) / 解像度: 2.87 Å 44026 9b03 EMDB-44026, PDB-9b03: INF2 in the Middle of F-Actin (Up state) 手法: EM (単粒子) / 解像度: 2.95 Å EMDB-44027: INF2 in the middle of F-actin, Down state consensus map 手法: EM (単粒子) / 解像度: 3.03 Å EMDB-44030: INF2 in the Middle of F-Actin, Down state WH2 from static FH2 手法: EM (単粒子) / 解像度: 3.13 Å EMDB-44031: INF2 in the Middle of an Actin Filament, Down state actin local refinement 手法: EM (単粒子) / 解像度: 2.94 Å EMDB-44032: INF2 in the Middle of Actin Filament, Down state mobile FH2 local refinement 手法: EM (単粒子) / 解像度: 3.22 Å EMDB-44033: INF2 in the Middle of F-Actin, Down state static FH2 local refinement 手法: EM (単粒子) / 解像度: 3.05 Å 44045 9b0k EMDB-44045, PDB-9b0k: INF2 in the Middle of F-Actin (Down state) 手法: EM (単粒子) / 解像度: 3.03 Å 44099 9b27 EMDB-44099, PDB-9b27: Dia1 at the Barbed End of F-Actin 手法: EM (単粒子) / 解像度: 3.51 Å 44135 9b3d EMDB-44135: Dia1 in the Middle of F-actin PDB-9b3d: mDia1 in the middle of F-actin 手法: EM (単粒子) / 解像度: 3.41 Å EMDB-44943: INF2 in the middle of F-Actin, Focus refinement for mobile WH2 手法: EM (単粒子) / 解像度: 3.26 Å EMDB-44948: INF2 in the Middle of F-actin, Up state mobile WH2 focus refinement 手法: EM (単粒子) / 解像度: 3.17 Å EMDB-44950: INF2 at the Barbed End of F-Actin, Local refinement for actin 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-44951: INF2 at the Barbed End of F-Actin, Leading FH2 focused refinement 手法: EM (単粒子) / 解像度: 4.38 Å EMDB-44956: INF2 at the Barbed END with Incoming Profilin-Actin, Consensus refinement 手法: EM (単粒子) / 解像度: 3.55 Å EMDB-44958: INF2 at the Barbed End of F-Actin with Incoming Profilin-Actin, Actin local refinement 手法: EM (単粒子) / 解像度: 3.48 Å EMDB-44972: INF2 at the Barbed End of F-Actin with Incoming Actin, Actin local refinement 手法: EM (単粒子) / 解像度: 3.82 Å EMDB-44973: INF2 at the Barbed End of F-Actin with Incoming Actin, Consensus refinement 手法: EM (単粒子) / 解像度: 3.82 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM
由来	homo sapiens (ヒト) oryctolagus cuniculus (ウサギ) mus musculus (ハツカネズミ)
キーワード	CYTOSOLIC PROTEIN / Actin / Filament / Elongation / Ends / Formin / Severing