EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	CryoEM structures reveal how the bacterial flagellum rotates and switches direction.
ジャーナル・号・ページ	Nat Microbiol, Vol. 9, Issue 5, Page 1271-1281, Year 2024
掲載日	2024年4月17日
著者	Prashant K Singh / Pankaj Sharma / Oshri Afanzar / Margo H Goldfarb / Elena Maklashina / Michael Eisenbach / Gary Cecchini / T M Iverson /
PubMed 要旨	Bacterial chemotaxis requires bidirectional flagellar rotation at different rates. Rotation is driven by a flagellar motor, which is a supercomplex containing multiple rings. Architectural ...Bacterial chemotaxis requires bidirectional flagellar rotation at different rates. Rotation is driven by a flagellar motor, which is a supercomplex containing multiple rings. Architectural uncertainty regarding the cytoplasmic C-ring, or 'switch', limits our understanding of how the motor transmits torque and direction to the flagellar rod. Here we report cryogenic electron microscopy structures for Salmonella enterica serovar typhimurium inner membrane MS-ring and C-ring in a counterclockwise pose (4.0 Å) and isolated C-ring in a clockwise pose alone (4.6 Å) and bound to a regulator (5.9 Å). Conformational differences between rotational poses include a 180° shift in FliF/FliG domains that rotates the outward-facing MotA/B binding site to inward facing. The regulator has specificity for the clockwise pose by bridging elements unique to this conformation. We used these structures to propose how the switch reverses rotation and transmits torque to the flagellum, which advances the understanding of bacterial chemotaxis and bidirectional motor rotation.
リンク	Nat Microbiol / PubMed:38632342 / PubMed Central
手法	EM (単粒子)
解像度	3.4 - 6.7 Å
構造データ	41100 8t8o EMDB-41100, PDB-8t8o: CCW Flagellar Switch Complex - FliF, FliG, FliM, and FliN forming 34-mer C-ring from Salmonella 手法: EM (単粒子) / 解像度: 4.0 Å 41101 8t8p EMDB-41101, PDB-8t8p: 33-mer FliF MS-ring from Salmonella 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-41102: 33-mer Flagellar Switch Complex - FliF, FliG, FliM and FliN forming C-ring from Salmonella 手法: EM (単粒子) / 解像度: 4.5 Å EMDB-41103: 35-mer Flagellar Switch Complex - FliF, FliG, FliM and FliN forming C-ring from Salmonella 手法: EM (単粒子) / 解像度: 4.5 Å EMDB-41104: 36-mer Flagellar Switch Complex - FliF, FliG, FliM and FliN forming C-ring from Salmonella 手法: EM (単粒子) / 解像度: 6.7 Å 43256 8vib EMDB-43256, PDB-8vib: CW Flagellar Switch Complex - FliF, FliG, FliM, and FliN forming single subunit of the C-ring from Salmonella 手法: EM (単粒子) / 解像度: 4.6 Å 43258 8vid EMDB-43258, PDB-8vid: CW Flagellar Switch Complex with extra density - FliF, FliG, FliM, and FliN forming single subunit of the C-ring from Salmonella 手法: EM (単粒子) / 解像度: 5.9 Å 43327 8vkq EMDB-43327, PDB-8vkq: CW Flagellar Switch Complex - FliF, FliG, FliM, and FliN forming the C-ring from Salmonella 手法: EM (単粒子) / 解像度: 4.6 Å 43328 8vkr EMDB-43328, PDB-8vkr: CW Flagellar Switch Complex with extra density - FliF, FliG, FliM, and FliN forming the C-ring from Salmonella 手法: EM (単粒子) / 解像度: 5.9 Å
由来	salmonella enterica subsp. enterica serovar typhimurium (サルモネラ菌)
キーワード	MOTOR PROTEIN / Domain Swap / Symmetry mismatch / Flagellar component / Switch complex / MS-ring / Membrane protein