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-Structure paper
タイトル | Convergent evolution in the supercoiling of prokaryotic flagellar filaments. |
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ジャーナル・号・ページ | Cell, Vol. 185, Issue 19, Page 3487-3500.e14, Year 2022 |
掲載日 | 2022年9月15日 |
著者 | Mark A B Kreutzberger / Ravi R Sonani / Junfeng Liu / Sharanya Chatterjee / Fengbin Wang / Amanda L Sebastian / Priyanka Biswas / Cheryl Ewing / Weili Zheng / Frédéric Poly / Gad Frankel / B F Luisi / Chris R Calladine / Mart Krupovic / Birgit E Scharf / Edward H Egelman / |
PubMed 要旨 | The supercoiling of bacterial and archaeal flagellar filaments is required for motility. Archaeal flagellar filaments have no homology to their bacterial counterparts and are instead homologs of ...The supercoiling of bacterial and archaeal flagellar filaments is required for motility. Archaeal flagellar filaments have no homology to their bacterial counterparts and are instead homologs of bacterial type IV pili. How these prokaryotic flagellar filaments, each composed of thousands of copies of identical subunits, can form stable supercoils under torsional stress is a fascinating puzzle for which structural insights have been elusive. Advances in cryoelectron microscopy (cryo-EM) make it now possible to directly visualize the basis for supercoiling, and here, we show the atomic structures of supercoiled bacterial and archaeal flagellar filaments. For the bacterial flagellar filament, we identify 11 distinct protofilament conformations with three broad classes of inter-protomer interface. For the archaeal flagellar filament, 10 protofilaments form a supercoil geometry supported by 10 distinct conformations, with one inter-protomer discontinuity creating a seam inside of the curve. Our results suggest that convergent evolution has yielded stable superhelical geometries that enable microbial locomotion. |
リンク | Cell / PubMed:36057255 / PubMed Central |
手法 | EM (らせん対称) / EM (単粒子) |
解像度 | 2.9 - 3.9 Å |
構造データ | EMDB-26995: Cryo-EM helical reconstruction of the EPEC H6 Curly I flagellar core EMDB-27008, PDB-8cvi: EMDB-27026, PDB-8cwm: EMDB-27029: Longer Cryo-EM Density map of the EPEC H6 Curly I bacterial flagellar filament EMDB-27059: Cryo-EM structure of the helical E. coli K12 flagellar filament core EMDB-27060, PDB-8cxm: EMDB-27064: Cryo-EM Helical Reconstruction of the EPEC H6 flagellar filament in the Normal waveform EMDB-27065: Cryo-EM helical reconstruction of the S. islandicus REY15A archaeal flagellar filament EMDB-27076, PDB-8cye: |
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キーワード | STRUCTURAL PROTEIN / Bacterial motility / flagellar filament / flagellin / Archaea / Motility / supercoiling / Bacterial flagellum |