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Yorodumi- EMDB-27064: Cryo-EM Helical Reconstruction of the EPEC H6 flagellar filament ... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-27064 | |||||||||
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Title | Cryo-EM Helical Reconstruction of the EPEC H6 flagellar filament in the Normal waveform | |||||||||
Map data | Cryo-EM Helical Reconstruction of the EPEC H6 flagellar filament in the Normal waveform | |||||||||
Sample |
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Biological species | Escherichia coli (E. coli) | |||||||||
Method | helical reconstruction / cryo EM / Resolution: 3.1 Å | |||||||||
Authors | Kreutzberger MAB / Chatterjee S / Frankel G / Egelman EH | |||||||||
Funding support | United States, 1 items
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Citation | Journal: Cell / Year: 2022 Title: Convergent evolution in the supercoiling of prokaryotic flagellar filaments. Authors: Mark A B Kreutzberger / Ravi R Sonani / Junfeng Liu / Sharanya Chatterjee / Fengbin Wang / Amanda L Sebastian / Priyanka Biswas / Cheryl Ewing / Weili Zheng / Frédéric Poly / Gad Frankel / ...Authors: Mark A B Kreutzberger / Ravi R Sonani / Junfeng Liu / Sharanya Chatterjee / Fengbin Wang / Amanda L Sebastian / Priyanka Biswas / Cheryl Ewing / Weili Zheng / Frédéric Poly / Gad Frankel / B F Luisi / Chris R Calladine / Mart Krupovic / Birgit E Scharf / Edward H Egelman / Abstract: The supercoiling of bacterial and archaeal flagellar filaments is required for motility. Archaeal flagellar filaments have no homology to their bacterial counterparts and are instead homologs of ...The supercoiling of bacterial and archaeal flagellar filaments is required for motility. Archaeal flagellar filaments have no homology to their bacterial counterparts and are instead homologs of bacterial type IV pili. How these prokaryotic flagellar filaments, each composed of thousands of copies of identical subunits, can form stable supercoils under torsional stress is a fascinating puzzle for which structural insights have been elusive. Advances in cryoelectron microscopy (cryo-EM) make it now possible to directly visualize the basis for supercoiling, and here, we show the atomic structures of supercoiled bacterial and archaeal flagellar filaments. For the bacterial flagellar filament, we identify 11 distinct protofilament conformations with three broad classes of inter-protomer interface. For the archaeal flagellar filament, 10 protofilaments form a supercoil geometry supported by 10 distinct conformations, with one inter-protomer discontinuity creating a seam inside of the curve. Our results suggest that convergent evolution has yielded stable superhelical geometries that enable microbial locomotion. | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_27064.map.gz | 87 MB | EMDB map data format | |
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Header (meta data) | emd-27064-v30.xml emd-27064.xml | 12.8 KB 12.8 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_27064_fsc.xml | 20.6 KB | Display | FSC data file |
Images | emd_27064.png | 35.4 KB | ||
Masks | emd_27064_msk_1.map | 926.9 MB | Mask map | |
Others | emd_27064_half_map_1.map.gz emd_27064_half_map_2.map.gz | 860.5 MB 860.5 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-27064 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-27064 | HTTPS FTP |
-Validation report
Summary document | emd_27064_validation.pdf.gz | 864 KB | Display | EMDB validaton report |
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Full document | emd_27064_full_validation.pdf.gz | 863.5 KB | Display | |
Data in XML | emd_27064_validation.xml.gz | 30 KB | Display | |
Data in CIF | emd_27064_validation.cif.gz | 39.7 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-27064 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-27064 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_27064.map.gz / Format: CCP4 / Size: 926.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Annotation | Cryo-EM Helical Reconstruction of the EPEC H6 flagellar filament in the Normal waveform | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.08 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
File | emd_27064_msk_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: Cryo-EM Helical Reconstruction of the EPEC H6 flagellar...
File | emd_27064_half_map_1.map | ||||||||||||
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Annotation | Cryo-EM Helical Reconstruction of the EPEC H6 flagellar filament in the Normal waveform | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Cryo-EM Helical Reconstruction of the EPEC H6 flagellar...
File | emd_27064_half_map_2.map | ||||||||||||
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Annotation | Cryo-EM Helical Reconstruction of the EPEC H6 flagellar filament in the Normal waveform | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Bacterial flagellar filament
Entire | Name: Bacterial flagellar filament |
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Components |
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-Supramolecule #1: Bacterial flagellar filament
Supramolecule | Name: Bacterial flagellar filament / type: complex / Chimera: Yes / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Escherichia coli (E. coli) / Strain: O127:H6 |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | helical reconstruction |
Aggregation state | filament |
-Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | TFS KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.2 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |