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-Structure paper
タイトル | Structural basis for the activation of the lipid scramblase TMEM16F. |
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ジャーナル・号・ページ | Nat Commun, Vol. 13, Issue 1, Page 6692, Year 2022 |
掲載日 | 2022年11月5日 |
著者 | Melanie Arndt / Carolina Alvadia / Monique S Straub / Vanessa Clerico Mosina / Cristina Paulino / Raimund Dutzler / |
PubMed 要旨 | TMEM16F, a member of the conserved TMEM16 family, plays a central role in the initiation of blood coagulation and the fusion of trophoblasts. The protein mediates passive ion and lipid transport in ...TMEM16F, a member of the conserved TMEM16 family, plays a central role in the initiation of blood coagulation and the fusion of trophoblasts. The protein mediates passive ion and lipid transport in response to an increase in intracellular Ca. However, the mechanism of how the protein facilitates both processes has remained elusive. Here we investigate the basis for TMEM16F activation. In a screen of residues lining the proposed site of conduction, we identify mutants with strongly activating phenotype. Structures of these mutants determined herein by cryo-electron microscopy show major rearrangements leading to the exposure of hydrophilic patches to the membrane, whose distortion facilitates lipid diffusion. The concomitant opening of a pore promotes ion conduction in the same protein conformation. Our work has revealed a mechanism that is distinct for this branch of the family and that will aid the development of a specific pharmacology for a promising drug target. |
リンク | Nat Commun / PubMed:36335104 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.93 - 3.49 Å |
構造データ | EMDB-15913, PDB-8b8g: EMDB-15914, PDB-8b8j: EMDB-15916, PDB-8b8k: EMDB-15917, PDB-8b8m: EMDB-15919, PDB-8b8q: EMDB-15958, PDB-8bc0: EMDB-15959, PDB-8bc1: |
化合物 | ChemComp-CA: ChemComp-P1O: |
由来 |
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キーワード | MEMBRANE PROTEIN / Lipid Transport / Lipid Scrambling / Ion Channel / Plasma Membrane / Blood Clotting / Exocytosis / Membrane Fusion / Lipid Scramblase / Blood Coagulation / Viral Entry / Cell Fusion |