EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Distinct structure and gating mechanism in diverse NMDA receptors with GluN2C and GluN2D subunits.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 30, Issue 5, Page 629-639, Year 2023
掲載日	2023年3月23日
著者	Jilin Zhang / Ming Zhang / Qinrui Wang / Han Wen / Zheyi Liu / Fangjun Wang / Yuhang Wang / Fenyong Yao / Nan Song / Zengwei Kou / Yang Li / Fei Guo / Shujia Zhu /
PubMed 要旨	N-methyl-D-aspartate (NMDA) receptors are heterotetramers comprising two GluN1 and two alternate GluN2 (N2A-N2D) subunits. Here we report full-length cryo-EM structures of the human N1-N2D di- ...N-methyl-D-aspartate (NMDA) receptors are heterotetramers comprising two GluN1 and two alternate GluN2 (N2A-N2D) subunits. Here we report full-length cryo-EM structures of the human N1-N2D di-heterotetramer (di-receptor), rat N1-N2C di-receptor and N1-N2A-N2C tri-heterotetramer (tri-receptor) at a best resolution of 3.0 Å. The bilobate N-terminal domain (NTD) in N2D intrinsically adopts a closed conformation, leading to a compact NTD tetramer in the N1-N2D receptor. Additionally, crosslinking the ligand-binding domain (LBD) of two N1 protomers significantly elevated the channel open probability (Po) in N1-N2D di-receptors. Surprisingly, the N1-N2C di-receptor adopted both symmetric (minor) and asymmetric (major) conformations, the latter further locked by an allosteric potentiator, PYD-106, binding to a pocket between the NTD and LBD in only one N2C protomer. Finally, the N2A and N2C subunits in the N1-N2A-N2C tri-receptor display a conformation close to one protomer in the N1-N2A and N1-N2C di-receptors, respectively. These findings provide a comprehensive structural understanding of diverse function in major NMDA receptor subtypes.
リンク	Nat Struct Mol Biol / PubMed:36959261
手法	EM (単粒子)
解像度	3.0 - 6.4 Å
構造データ	33788 7yff EMDB-33788, PDB-7yff: Structure of GluN1a-GluN2D NMDA receptor in complex with agonist glycine and competitive antagonist CPP. 手法: EM (単粒子) / 解像度: 3.6 Å 33789 7yfg EMDB-33789, PDB-7yfg: Structure of the Rat GluN1-GluN2C NMDA receptor in complex with glycine and glutamate (major class in asymmetry) 手法: EM (単粒子) / 解像度: 3.6 Å 33790 7yfh EMDB-33790, PDB-7yfh: Structure of the Rat GluN1-GluN2C NMDA receptor in complex with glycine, glutamate and (R)-PYD-106 手法: EM (単粒子) / 解像度: 3.0 Å 33791 7yfi EMDB-33791, PDB-7yfi: Structure of the Rat tri-heteromeric GluN1-GluN2A-GluN2C NMDA receptor in complex with glycine and glutamate 手法: EM (単粒子) / 解像度: 3.3 Å 33792 7yfl EMDB-33792, PDB-7yfl: Structure of GluN1a-GluN2D NMDA receptor in complex with agonists glycine and glutamate. 手法: EM (単粒子) / 解像度: 3.9 Å 33793 7yfm EMDB-33793, PDB-7yfm: Structure of GluN1b-GluN2D NMDA receptor in complex with agonists glycine and glutamate. 手法: EM (単粒子) / 解像度: 5.1 Å 33795 7yfo EMDB-33795, PDB-7yfo: Structure of GluN1a E698C-GluN2D NMDA receptor in cystines crosslinked state. 手法: EM (単粒子) / 解像度: 6.4 Å 33798 7yfr EMDB-33798, PDB-7yfr: Structure of GluN1a E698C-GluN2D NMDA receptor in cystines non-crosslinked state. 手法: EM (単粒子) / 解像度: 5.1 Å 34674 8hdk EMDB-34674, PDB-8hdk: Structure of the Rat GluN1-GluN2C NMDA receptor in complex with glycine and glutamate (minor class in symmetry) 手法: EM (単粒子) / 解像度: 4.3 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン ChemComp-GLY: GLYCINE / グリシン ChemComp-7RC: (2R)-4-(3-phosphonopropyl)piperazine-2-carboxylic acid / (-)-CPP ChemComp-GLU: GLUTAMIC ACID / グルタミン酸 ChemComp-IWB: methyl 4-[(2~{R})-3-ethanoyl-1-[2-(2-methyl-1~{H}-indol-3-yl)ethyl]-4-oxidanyl-5-oxidanylidene-2~{H}-pyrrol-2-yl]benzoate
由来	homo sapiens (ヒト) rattus norvegicus (ドブネズミ)
キーワード	ELECTRON TRANSPORT / ion channel / cryo-EM structure / glutamate receptor / synaptic protein / MEMBRANE PROTEIN / NMDA receptor / GluN2C / GluN2A